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Protective effect of angelica sinensis polysaccharide on the thymus structure and function of aging model rats
JIANG Rong ZHANG Meng-si JIA Dao-yong ZHANG Yan-yan XIA Jie-yu ZOU Ting YE Jian-wen WANG Lu WANG Ya-ping*
Acta Anatomica Sinica ›› 2016 ›› Issue (2) : 254-260.
Protective effect of angelica sinensis polysaccharide on the thymus structure and function of aging model rats
Objective To investigate the protective effect of Angelica Sinensis polysaccharide (ASP)on the thymus structure and function of aging model rats and its relative mechanism. Methods Forty SD rats were randomly divided into 4 groups. Aging model group were injected with D-galactose ［120mg/(kg〖DK〗·d) ］ for 42 days by subcutaneous way. ASP aging group were also given D-galactose with the same dose and time as aging model group, and from the 14th day on, rats were maintained with ASP (100 mg/kg) by intra-peritoneal way for 28 days. Normal control group were received saline with the same volume for 42 days. ASP control group were given saline with the same volume for 14 days, and received ASP (100 mg/kg) for 28 days by intra-peritoneal way. After 2 days of injections, the thymus index and thymus weight were measured, and paraffin sections were made to observe thymus microscopic structures. The senescence of thymus and the proliferative capacity and cell cycles of thymocytes were measured. The content of cytokines and the production of reactive oxygen species (ROS) and superoxide dismutase (SOD) were detected. Results The thymus structure and function were obviously induced senescence by treating with D-galactose. Comparing the ASP aging group with the aging model group, thymus index, thymus weight, thymus cortex area proportion, the proliferative capacity and proportion of S phase of thymocytes, thymocytes secretary capability of interleukin(IL)-2, tumor necrosis factor (TNF-α), granulocyte-macrophage colony-stimulating factor (GM-CSF), and IL-6, the active content of SOD were obviously increased. The percentage of senescence β-galactosidase (SA-β-Gal) positive thymocytes, and the production of ROS were significantly decreased. Conclusion Angelica Sinensis polysaccharide has a significantly antiaging or protective effect on thymus injury. It is suggested that the mechanism may be ASP inhibiting oxidative stress.
［1］Wagner WM, Ouyang Q, Sekeri-Pataryas K, et al.Basic biology and clinical impact of immunosenescence ［J］. Biogerontology, 2004,5(1):63-66.
［2］Cao W, Li XQ, Wang X, et al. A novel polysaccharide, isolated from Angelical sinensis (Oliv.) Diels induces the apoptosis of cervical cancer HeLa cells through an intrinsic apoptosis pathway［J］. Phytomedicine, 2010,17 (8-9):598-605.
［3］Liu C, Li J, Meng FY, et al. Polysaccharides from the root of Angelica sinensis promotes hematopoiesis and thrombopoiesis through the P13K/AKT pathway［J］.BMC Complement Altern Med,2010,21(10):79.
［4］He XL，Guan XJ, Wu H, et al. Effects of Angelica polysaccharide on the apoptosis and oxidative damage in bone marrow mononuclear cells of ionizing radiation injured mice［J］.Joumal of Chongqing Medical University,2012,37(40): 315-319.(in Chinese)
何晓莉，关雪晶，吴宏，等.当归多糖对电离辐射致小鼠骨髓单个核细胞凋亡及氧化损伤的影响［J］.重庆医科大学学报,2012,37(40):315-319.
［5］Zhang XP, Liu J, Xu ChY, et al. Effect of Angelica sinensis polysaccharide on expression of telomere,telomerase and P53 in mice aging hematopoietic stem cells［J］.China Journal of Chinese Materia Medica,2013,38(14):2354-2358.(in Chinese)
张先平，刘俊，徐春燕，等.当归多糖对小鼠衰老造血干细胞端粒、端粒酶及P53的影响［J］.中国中药杂志，2013,38(14):2354-2358.
［6］Zhang XP, Wang QX, Chen B,et al.Angelica sinensis polysaccharides regulate aging of mice hematopoietic stem cell through cell cycle protein［J］.Basic and Clinical Medicine,2013,33(3):321-324.(in Chinese)
张先平,王乾兴,陈斌,等.当归多多糖对小鼠衰老造血干细胞周期蛋白的调控［J］.基础医学与临床，2013,33（3）：321-324.
［7］Zhang XP, Wang QX, Chen B, et al. Angelica sinensis polysaccharides delay aging of hematopoietic stem cells through inhibitting oxidative damge［J］. China Journal of Chinese Materia Medica,2013,38(3):407-412.(in Chinese)
张先平,王乾兴,陈斌,等.当归多糖抑制氧化损伤延缓造血干细胞衰老［J］.中国中药杂志，2013,38(3):407-412.
［8］Peng B, Chen MSh, Pu Y, et al. Antiaging effects of Ginsenoside Rg1 and it’s mechanisms on brain aging rats induced by D-galactose［J］. Joumal of Chongqing Medical University,2011,36(4):419-422. (in Chinese)
彭彬,陈茂山,蒲莹,等.人参皂苷Rgl延缓D-半乳糖大鼠脑衰老作用及机制的初步研究［J］.重庆医科大学学报,2011,36(4):419-422.
［9］Gorczynski RM, Terzioglu E. Aging and the immune system［J］.Int Urol Nephrol, 2008,40(4): 1117-1125.
［10］Weng NP. Aging of the immune system: how much can the adaptive immune system adapt［J］. Immunity, 2006, 24(5):495-499.
［11］Dai DF,Rabinovitch PS,Ungvari Z.Mitochondria and cardiovascular aging［J］.Circ Res,2012, 110(8): 1109-1124.
［12］Droge W. Free radicals in the physiological control of cell function［J］.Physiol Rev,2002, 82 (1):47-95
［13］Takeuchi A,Mishina Y,Miyaishi O, et al. Heterozygosity with respect to Zfp 148 causes complete loss of fetal germ cells during mouse embryogenesis［J］.Nat Genet,2003,33(2):172 -176.
［14］Simon HU,Haj-Yehia A,Levi-Schaffer F. Role of reactive oxygen species(ROS)in apoptosis induction［J］. Apoptosis,2000,5(5):415-418.
［15］Szibor M,Richter C,Ghafourifar P.Redox control of mitochondrial functions［J］.Antioxid Redox Signal, 2001,3(3):515-523.
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