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Expression and mechanism of activated leukocyte cell adhesion molecule, E-cadherin and β-catenin in papillary thyroid carcinoma
SUN Lin ZHANG Wen-jing ZHANG Jing WU Jing-fang*
Acta Anatomica Sinica ›› 2016 ›› Issue (2) : 221-227.
Expression and mechanism of activated leukocyte cell adhesion molecule, E-cadherin and β-catenin in papillary thyroid carcinoma
Objective To detect the expression of activated leukocyte cell adhesion molecule (ALCAM), E-cadherinand β-catenin in the development of papillary thyroid carcinoma（PTC）,nodular goiter（NG）and healthy persons and to explore the mechanisms of ALCAM, E-cadherin and β-catenin in PTC. Methods The expressions of ALCAM,E-cadherin and β-catenin proteins in PTC were detected by Western blotting and immunohistochemical SP method, and the relationships with the clinical pathology characteristics were analyzed. Results The positive expression of ALCAM in the tissue of PTC was significantly higher than that in the normal tissue adjacent to carcinoma and in the tissue of NG(P＜0.05). The positive expression of E-cadherin in the tissue of PTC was significantly lower than that in the normal tissue adjacent to carcinoma and in the tissue of NG(P＜0.05). The ectopic expression rate of β-catenin in the tissue of PTC was significantly higher than in the tissue of NG and the normal tissue adjacent to carcinoma (P＜0.05). The expression of ALCAM, E-cadherin and β-catenin in the tissue of nodular goiter was not different from the normal tissue adjacent to carcinoma(P＞0.05). The abnormal expressions of ALCAM, E-cadherin and β-catenin were unrelated to the age, sex and size of the tumor (P>0.05) of PTC patients, but associated with the lymph node metastasis (P<0.05).
Conclusion The expressions of ALCAM and β-catenin are stronger in PTC tissues than in NG. E-cadherin was little expression in the tissue of PTC. The abnormal expressions of ALCAM, E-cadherin and β-catenin have the positive correlation with the lymph node metastasis.They play an important role in the process of PTC, including the occurrence, development,invision and metastasis.
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