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Expression difference of surface markers between adipose derived mesenchymal stem cells and bone marrow mesenchymal stem cells of the mouse
YU XiaLI Qiong REN Ming-fen NIU Yan-fei GAN Li GUO Zhi-kun*
Acta Anatomica Sinica ›› 2016 ›› Issue (2) : 203-208.
Expression difference of surface markers between adipose derived mesenchymal stem cells and bone marrow mesenchymal stem cells of the mouse
Objective To investigate the expression difference of surface markers of mouse mesenchymal stem cells from adipose tissue and bone marrow. Methods Mouse adipose-derived mesenchymal stem cells（ADMSCs）and bone marrow derived mesenchymal stem cells(BMSCs) were induced to differentiate into osteoblasts, adipocytes and cardiomyocytes and then indentified their differentiation in vitro. The expressions of CD29，CD44，CD45 and CD73 were detected and compared by flow cytometry and immunofluorescence. Results The morphology of BMSCs was uniform，and most of them were spindle. While ADMSCs showed multiplicity，presenting spindle, starlike and polygonal on which were much excretion，and their size was large.Both cells differentiated to osteoclasts, adipocytes and cardiomyocytes-like cells.The result of flow cytometry showed BMSCs were positive for CD29（83.43±1.97）%，CD44（90.33±0.81）%and CD73（2.63±0.42）%；ADMSCs were positive for CD29（53.1±1.05）%，CD44（34.8±2.1）%，while the expression of CD73 was unstable from 1.8% to 19.7%.Both were negative for CD45.The result of Immunofluorescence indicated the co-expression of CD73 and CD29，CD44. Conclusion Both BMSCs and ADMSCs have the potential to differentiate into adipose，osteogenesis，and myocardial muscle.Mouse BMSCs and ADMSCs are different in the cells morphology and the expression of the surface marker；both highly expressed CD29 and CD44，but BMSCs have consistently high expression of CD29 and CD44 compared with ADMSCs.On the contrary，the expression of CD73 is significantly lower on both cells and unstable on ADMSCs.
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