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Effect of dexamethasone on the proliferation and apoptosis of rat liver cell line BRL-3A
CHANG Cui-fangZHAO Wei-mingYANG JingLI Xiao-fangCHEN Sha-shaWANG Gai-pingXU Cun-shuan*
Acta Anatomica Sinica ›› 2016 ›› Issue (2) : 197-202.
Effect of dexamethasone on the proliferation and apoptosis of rat liver cell line BRL-3A
Objective To explore the effect of dexamethasone on cell proliferation and apoptosis in the rat liver cell line BRL-3A in vitro.Methods BRL-3A cells were treated with different concentrations of dexamethasone, and MTT method was used to observe the effect of dexamethasone on cell activity at 12, 24, 48, 72 and 120 hours after treatment. Annexin V-FITC staining and propidium iodide(PI) staining were used to detect the effect of cell apoptosis and cell cycle. Real-time PCR was used to evaluate the changes in the expression of related genes. Results The result of MTT assays revealed that dexamethasone inhibited the proliferation of BRL-3A cells in a dose-dependent manner. Annexin V-FITC staining showed that dexamethasone significantly induced the apoptosis of BRL-3A. PI staining indicated that the ability of proliferation decreased in the cells treated with dexamethasone. Real-time PCR analysis showed that pro-apoptosis genes Caspase-3, Caspase-8 and Caspase-9 were up-regulated, while pro-proliferation genes Ccnd1 and Jun were down-regulated. Conclusion Dexamethasone may inhibit the proliferation of BRL-3A cell line and induce its apoptosis by up-regulating Caspase-3, Caspase-8 and Caspase-9 and down-regulating the expression of Ccnd1 and Jun.
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