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Expression changes of c-Fos and neuronal nitric oxide synthase in medullary visceral zone induced by varied distention of uterine cervicx
SHEN Liang-hua WU Zhong-min ZHENG Chun-hua SUN Ting-ting LI Wei-yun JIAO Cui-cui CHEN Xin-zhong LING Shu-cai*
Acta Anatomica Sinica ›› 2016 ›› Issue (2) : 159-165.
Expression changes of c-Fos and neuronal nitric oxide synthase in medullary visceral zone induced by varied distention of uterine cervicx
Objective To investigate the expression changes of neuronal nitric oxide synthase (nNOS) and c-Fos in the rat medullary visceral zone with different uterine cervical distension(UCD),of which the transferring mechanism in the medulla oblongata is still unclear. Methods Adult female Sprague-Dawley rats were randomly divided into the control group (UCD 0g), 50g tension group (UCD 50g) and 100 g tension group (UCD 100g). The UCD model was established through distension forces generated by hanging different standard weights (50g,100g) at the end of metal crooks. The distribution of nNOS, c-Fos and c-Fos/nNOS positive neurons was observed 2 hours after UCD by NADPH histochemistry and c-Fos immunohistochemistry. The protein expression of nNOS and c-Fos in the medulla oblongata was detected by Western blotting. The mRNA expression of nNOS and c-Fos in the medulla oblongata was detected by Real-time PCR.
Results c-Fos immunoreactive neurons in nucleus were brown, round or oval, while the cytoplasm was unstained. The cell bodies and processes of nNOS positive neurons were blue, and the nuclei were not stained in the form of a bubble. c-Fos/nNOSdouble labeled cell bodies and dendrites of neurons stained blue, and nucleus showed brown yellow. These neurons were mainly distributed in the nucleus of the solitary tract nucleus (NTS) and the lateral reticular nucleus (LRN). Compared with UCD 0 g group, the number of nNOS, c-Fos and c-Fos/nNOS positive neurons in the NTS and LRN of UCD 50 g group and UCD 100 g group were significantly increased, and the dyeing significantly deepened. The protein and mRNA levels of c-Fos, nNOS in UCD 100 g group were higher than that of UCD 50 g group (P<0.05). And both of the two groups were significantly elevated (P<0.01) as compared with UCD 0 g group. Conclusion Acute uterine cervical dilatation in rats can lead to increased expression of c-Fos and nNOS in NTS and LRN of the medulla oblongata, nNOS positive neurons in NTS and LRN may be involved in the conduction of the UCD pain in the medulla oblongata.
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