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Effect of intervention of follicle-stimulating hormone/ follicle-stimulating hormone and luteinizing hormone on the expression of vascular endothelial growth factor in the vitrification mouse ovary
CHEN Jie CHANG Qing YANG Yan-zhou PEI Xiu-ying HEI Chang-chun YU Jia SUN Miao WANG Yan-rong*
Acta Anatomica Sinica ›› 2015, Vol. 46 ›› Issue (6) : 819-823.
Effect of intervention of follicle-stimulating hormone/ follicle-stimulating hormone and luteinizing hormone on the expression of vascular endothelial growth factor in the vitrification mouse ovary
Objective To investigate the best way of hormone intervention for protecting the vitrification ovarian tissues via observation of the morphological changes of ovary and the expression of vascular endothelial growth factor (VEGF）after the intervention by follicle-stimulating hormone（FSH）/FSH+ luteinizing hormone (LH）in the whole process of vitrification. Methods Four-week-old C57BL/6J mice were divided into four groups:the fresh control group(CG),the vitrification control group(VCG), the vitrification group intervention by 300 IU/L FSH(OG-FSH) and the vitrification group intervention by 150 IU/L FSH+150 IU/L LH(OG-FSH+LH),30 ovary samples per group. The changes of morphological structure of ovary,the expression of VEGF protein and mRNA in every group were observed and analyzed using routine histology, Western blotting and Real-time PCR. Results The percentage of normal follicles was the highest in OG-FSH+LH group,and significantly higher in OG-FSH+LH group than in OG-FSH group (P<0.05). The expression of VEGF protein was higher in OG-FSH+LH group than OG-FSH group(P<0.05).The order of intensities of expression for VEGF mRNA was OG-FSH+LH group> in OG-FSH group>in VCG group (P<0.05). Conclusion The best intervention way is FSH+LH interpose in the whole process of vitrification, which is in favour of the expression of VEGF.
Follicle-stimulating hormone / Luteinizing hormone / Vascular endothelial growth factor / Western blotting / Real-time PCR / Mouse
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