Radiosensitivity on A549 cells by dihydromyricetin pre-intervention in vitro

SUN Da-yong BI Hong-dong LIANG Hong-hu ZUO Yan-zhen*WANG Ai-min JIAO Hai-tao

doi:10.16098/j.issn.0529-1356.2015.06.012

Acta Anatomica Sinica ›› 2015, Vol. 46 ›› Issue (6) : 797-801. DOI: 10.16098/j.issn.0529-1356.2015.06.012

Radiosensitivity on A549 cells by dihydromyricetin pre-intervention in vitro

SUN Da-yong¹BI Hong-dong² LIANG Hong-hu¹ ZUO Yan-zhen^2*WANG Ai-min³ JIAO Hai-tao⁴

Author information +

History +

Abstract

Objectiv To study the radiosensitivity of dihydromyricetin (DMY) on human non-small cell lung cancer A549 cells, and to explore a new drug for lung cancer radiotherapy sensitization.Methods MTT assay and colony formation assay were used to determined the IC₂₀ of dihydromyricetin at different density A549 cells. Flow cytometry (FCM) was used to detect the change of A549 cell cycle phase impacted by DMY of IC₂₀during different time (6, 12, 24, 48hours). Colony formation assay was used to determine the radiosensitivity on A549 cells by dihydromyricetin pre-intervention in vitro. Results The proliferation of A549 cells was inhibited in a dose-dependent manner, MTT assay showed the IC20 was 40mg/L and colony formation assay showed the IC₂₀was 7mg/L. FCM cycle experiment showed that the rate of G2/M phase ratio obviously increased when A549 cells was incubated 24 hours with DMY；A significant sensibilization leading by the effect of radiotherapy sensibilization on A549 cells after DMY pre-intervention 24 hours was not detected by clone formation sensibilization assay.Conclusion The proliferation of A549 cells was inhibited in a dose-dependent manner of dihydromyricetin, and the effect was influenced by cell density. Although the rate of G₂/M phase ratio obviously increased when A549 cells were incubated 24 hours with DMY, there was no significant sensibilization on A549 cells after DMY pre-intervention 24 hours. A further study should be made.

Key words

Dihydromyricetin / A549 cell / Radiosensitivity, Sensibilization / Flow cytometry / Human

Cite this article

EndNote

Ris (Procite)

Bibtex

Download Citations

SUN Da-yong BI Hong-dong LIANG Hong-hu ZUO Yan-zhen*WANG Ai-min JIAO Hai-tao. Radiosensitivity on A549 cells by dihydromyricetin pre-intervention in vitro[J]. Acta Anatomica Sinica. 2015, 46(6): 797-801 https://doi.org/10.16098/j.issn.0529-1356.2015.06.012

References

［1］Zhao WY, Sun XCh. Progression of tumor radiosensitization［J］. Chinese Clinical Oncology, 2012, (7): 655-659. (in Chinese)
赵维勇, 孙新臣. 放射治疗增敏的研究现状［J］. 临床肿瘤学杂志, 2012, (7): 655-659.
［2］Cen JH. Study on apoptosis-inducing mechanism of dihydromyricetin on human liver cancer HepG2［J］. The Journal of Practical Medicine, 2014, (11): 1712-1715. (in Chinese)岑钧华. 二氢杨梅素诱导肝癌细胞株HepG2凋亡途径的探讨［J］. 实用医学杂志, 2014, (11): 1712-1715.
［3］Hou XL, Wang WQ, Shi ChY, el al. Research progress in pharmacological effects of dihydromyricelin［J］. Chinese Traditional and Herbal Drugs, 2015, 46(4): 603-609. (in Chinese)
侯小龙, 王文清, 施春阳,等. 二氢杨梅素药理作用研究进展［J］. 中草药, 2015, 46(4): 603-609.
［4］Yu CC, Hung SK, Liao HF, et al. RAD001 enhances the radiosensitivity of SCC4 oral cancer cells by inducing cell cycle arrest at the G2/M checkpoint［J］. Anticancer Res, 2014,34(6): 2927-2935.
［5］Zhou FZh, Zhang XY, Guo Y. Research progress on antitumor effect and mechanisms of dihydromyricetin［J］. Chinese Archives of Traditional Chinese Medicine, 2010, 28(9):1914-1916. (in Chinese)
周防震, 张晓元, 郭勇. 二氢杨梅素抗肿瘤活性及其机制的研究进展［J］. 中华中医药学刊, 2010,28(9):1914-1916.
［6］Zhou FZh, Zhang XY, Sun FY, et al. Anti proliferation effect of dihydromyricetin on MDA-MB-231 cell in vitro［J］. Cancer Research on Prevention and Treatment, 2012, 39(1): 95-97. (in Chinese)
周防震, 张晓元, 孙奋勇, 等. 二氢杨梅素对人乳腺癌细胞MDA-MB-231的体外抗增殖作用［J］. 肿瘤防治研究, 2012, 39(1): 95-97.
［7］Franken NA, Rodermond HM, Stap J, et al. Clonogenic assay of cells in vitro［J］. Nat Protoc, 2006,1(5):2315-2319.
［8］Yuan ZhY, Liu XB, Zhuang HQ, et al. Radiosensitizing effects of h-R3 with different time series of administration on human lung cancer cells expressing various EGFR levels［J］. Chinese Journal of Clinical Oncology, 2011,(17):1019-1023. (in Chinese)
袁智勇, 刘晓斌, 庄洪卿, 等. 尼妥珠单抗不同给药时序对人肺癌细胞系的放射增敏作用［J］. 中国肿瘤临床, 2011,(17):1019-1023.
［9］Zhang XY, Wu H, Lin X, et al. Scavenging effect of dihydromyricetin on the free radicals by ESR ［J］. Modern Food Science and Technology, 2010,26(10):1040-1042,1070.
张晓元, 吴晖, 林霞, 等. 用ESR检测二氢杨梅素对自由基的清除作用的研究［J］. 现代食品科技, 2010,26(10):1040-1042,1070.
［10］Gu XZh, Yin WB, Yu ZH, et al. Radiation Oncology［M］. 4th ed. Peking: China Union Medical College Press, 2007: 227-228. (in Chinese)
谷铣之, 殷蔚伯, 余子豪, 等. 肿瘤放射治疗学［M］. 第4版, 北京: 中国协和医科大学出版社, 2007: 227-228.
［11］Song MJ, Liu H, Shen YL. The research progress of flavonoids radiosensitization agents［J］. Chinese Journal of Hospital Pharmacy, 2013, (12): 997-999, 1002. (in Chinese)宋梦姣, 刘宏, 申玉莉. 黄酮类放射增敏剂的研究进展［J］. 中国医院药学杂志, 2013, (12): 997-999, 1002.
［12］Hermann RM, Fest J, Christiansen H, et al. Radiosensitization dependent on p53 function in bronchial carcinoma cells by the isoflavone genistein and estradiol in vitro［J］. Strahlenther Onkol, 2007,183(4):195-202.