Welcome to visit Acta Anatomica Sinica! Today is
Chinese
Functions of Wnt-10a during K562 cell differentiation induced by hemin
WANG Jie HU Jiang-jiang ZHAO Fu-kun ZHANG Shi-fu*
Acta Anatomica Sinica ›› 2015, Vol. 46 ›› Issue (6) : 772-779.
Functions of Wnt-10a during K562 cell differentiation induced by hemin
Objective To study expression changes and functions of Wnt-10a protein in K562 differentiation induced by hemin. Methods Benzidine staining was used to test hemininduced erythroid differentiation of K562 cells. Western blotting and immunocytochemistry were used to detect protein expression changes of Wnt-10a and protein localization in K562 cells. Real-time PCR and Real-time PCR were applied to investigate mRNA expression of key proteins in Wnt signalling pathway during K562 defferentiation process. Wnt signaling pathway activator and Wnt-10a high expression K562 cell line were used to explore the influence of altered Wnt pathway. Results Wnt-10a protein and mRNA expressions were upregulated after a brief down in the erythroid differentiation process of K562 cells induced by hemin when, Wnt-10a protein migrated to the cell membrane. The key factors of the main Wnt signaling pathways changed during K562 differentiation. By using Wnt signaling pathway inhibitor, cell proliferation capability was enhanced during K562 differentiation by hemin. When Wnt-10a was highly expressed, the ability of induced differentiation of K562 cells was improved. Conclusion These results show that -10a protein is closely related with K562 cell differentiation induced by hemin, which may offer clues for further study of the mechanism of leukemia and cell differentiation.
Wnt-10a / K562 / Hemin / Erythroid / Differentiation / Western blotting / Human
［1］Lozzio CB, Lozzio BB. Human chronic myelogenous leukemia cell-line with positive philadelphia chromosome［J］. Blood, 1975, 45(3): 321-334.
［2］Huo XF, Yu J, Peng H, et al. Differential expression changes in k562 cells during the hemin-induced erythroid differentiation and the phorbol myristate acetate (pma)-induced megakaryocytic differentiation［J］. Mol Cell Biochem, 2006, 292(1-2): 155-167.
［3］Nakajima O, Iwasaki S, Hashimoto Y. Hemin-induced erythroid differentiation of human myeloleukemia K562 cell line and its modification by bioresponse modifiers［J］. Cell Mol Biol, 1997, 43(1): 115-134.
［4］Witt O, Sand K, Pekrun A. Butyrate-induced erythroid differentiation of human k562 leukemia cells involves inhibition of erk and activation of p38 map kinase pathways［J］. Blood, 2000, 95(7): 2391-2396.
［5］Kamano H, Tanaka T, Ohnishi H, et al. Effects of the antisense myb expression on hemin-and erythropoietin-induced erythroid differentiation of k562 cells［J］. Biochem Mol Biol Int, 1994, 34(1): 85-92.
［6］Woon Kim Y, Kim S, Geun Kim C, et al. The distinctive roles of erythroid specific activator gata-1 and nf-e2 in transcription of the human fetal gamma-globin genes［J］. Nucleic Acids Res, 2011, 39(16): 6944-6955.
［7］Chan JY, Han XL, Kan YW. Isolation of cdna encoding the human nf-e2 protein［J］. Proce Nati Acad Sci USA, 1993, 90(23): 11366-11370.
［8］Lento W, Congdon K, Voermans C, et al. Wnt signaling in normal and malignant hematopoiesis［J］. Cold Spring Harb Perspect Biol, 2013, 5(2): 1-10
［9］Wolda SL, Moon RT. Cloning and developmental expression in xenopus laevis of seven additional members of the wnt family［J］. Oncogene, 1992, 7(10): 1941-1947.
［10］Kelly GM, Lai CJ, Moon RT. Expression of wnt10a in the central nervous system of developing zebrafish［J］. Dev Biol, 1993, 158(1): 113-121.
［11］Gelebart P, Anand M, Armanious H, et al. Constitutive activation of the wnt canonical pathway in mantle cell lymphoma［J］. Blood, 2008, 112(13): 5171-5179.
［12］Bohring A, Stamm T, Spaich C, et al. WNT10A mutations are a frequent cause of a broad spectrum of ectodermal dysplasias with sex-biased manifestation pattern in heterozygotes［J］. Am J Hum Genet, 2009, 85(1): 97-105.
［13］Logan CY, Nusse R. The Wnt signaling pathway in development and disease［J］. Annu Rev Cell Dev Biol, 2004, 20(1)781-810.
［14］Gao B, Song H, Bishop K, et al. Wnt signaling gradients establish planar cell polarity by inducing vangl2 phosphorylation through ror2［J］. Dev Cell, 2011, 20(2): 163-176.
［15］Montcouquiol M, Crenshaw EB, Kelley MW. Noncanonical wnt signaling and neural polarity［J］. Ann Rev Neurosci, 2006, 29(1)363-386.
［16］Austin TW, Solar GP, Ziegler FC, et al. A role for the wnt gene family in hematopoiesis: expansion of multilineage progenitor cells［J］. Blood, 1997, 89(10): 3624-3635.
［17］Tran HT, Sekkali B, Van Imschoot G, et al. Wnt/beta-catenin signaling is involved in the induction and maintenance of primitive hematopoiesis in the vertebrate embryo［J］. Proc Nati Acad Sci USA, 2010, 107(37): 16160-16165.
［18］Van Den Berg DJ, Sharma AK, Bruno E, et al. Role of members of the wnt gene family in human hematopoiesis［J］. Blood, 1998, 92(9): 3189-3202.
［19］Dijksterhuis JP, Petersen J, Schulte G. Wnt/frizzled signalling: Receptor-ligand selectivity with focus on fzd-g protein signalling and its physiological relevance: Iuphar review 3［J］. Br J Pharmacol, 2014, 171(5): 1195-1209.
［20］Miller JR. The Wnts［J］. Genome Biol, 2002, 3(1): REVIEWS3001. Epub 2001 Dec 28.
［21］Plaisancie J, Bailleul-Forestier I, Gaston V, et al. Mutations in WNT10A are frequently involved in oligodontia associated with minor signs of ectodermal dysplasia［J］. Am J Med Gene A, 2013, 161A(4): 671-678.
［22］Vink CP, Ockeloen CW, ten Kate S, et al. Variability in dentofacial phenotypes in four families with wnt10a mutations［J］. Eur J Hum Genet, 2014, 22(9): 1063-1070.
/
| 〈 |
|
〉 |
