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Therapeutic effect of neuregulin1β on the dysfunction of learning and memory in rats with chronic omethoate poisoning
RONG Li-xia SUN Shan-shan DING Kun ZHANG Rui ZHANG Mei-zeng*
Acta Anatomica Sinica ›› 2015, Vol. 46 ›› Issue (6) : 750-756.
Therapeutic effect of neuregulin1β on the dysfunction of learning and memory in rats with chronic omethoate poisoning
Objective To observe the effect of neuregulin1β (NRG1β) on the level of phosphorylated extracellular regulated protein kinase(ERK)1/2 (p-ERK1/2), and explore the therapeutic mechanism of NRG1β on the cognitive dysfunction in rats with chronic omethoate poisoning. Methods Rats with strong learning and memory ability were selected by Y-electric maze test to establish the cognitive impairment model by subcutaneous injection of omethoate［5mg/(kg·d)］ for 4 weeks. After the model was established, rats in the treatment group were injected with NRG1β into their ventricles. The cognitive capacity of rats was evaluated with Y-electric maze. The morphology and ultrastructure of hippocampus were observed by haematoxylin eosin (HE) staining and transmission electron microscopy (TEM) respectively. The expression of p-ERK1/2 was determined by immunohistochemical (IHC) staining and Western blotting. Results Y-electric maze test showed the cognitive ability in rats with omethoate exposed (model and treatment groups) reduced significantly compared with normal rats, but the ability of treated rats was improved obviously compared with that of model rats. In compare with normal rats, obvious injury of hippocampus was observed in the model group by HE staining and TEM, and the phosphorylation level of ERK1/2 decreased significantly. In the rats treated with NRG-1β, the injury was milder than the model rats, and the phosphorylation level of ERK1/2 increased significantly. Conclusion In the chronic omethoate poisoning rat, NRG-1β can promote the phosphorylation level of ERK1/2 in hippocampus, and may play an important role in the improvement of cognitive function.
Neuregulin 1β / Omethoate / Cognitive dysfunction / Extracellular regulated protein kinase / Western blotting / Rat
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