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Cortical lamination and cellular polarization in reeler mice
MU Xiao-yun LI Yong-qiang LIANG Shuang SHI Shu-qin FU Su DENG Jin-bo*
Acta Anatomica Sinica ›› 2015, Vol. 46 ›› Issue (6) : 721-728.
Cortical lamination and cellular polarization in reeler mice
Objective To investigate the role of Reelin in the process of cortical lamination, cell differentiation and polarization as well as the relevant regulatory effects of Reelin signaling pathway. Methods A total of 96 wild-type (WT) mice and reeler mice from embryonic day 17(E17) to postnatal day 21(P21) were used in this study. The radial glial cells, neural stem cells, and polarity protein were visualized by utilizing immunofluorescent labeling, Nissl staining，5-bromo-deoxyuridine(BrdU) assay and DiI tracing method. The polarity of pyramidal cells in cerebral cortex and hippocampus were also measured. Results Comparing with WT mice, the cortical lamination was disordered due to the lack of Reelin in reeler mice. The orientations of radial glial cells were disorder accompanied with number decreases. BrdU positive cells were arranged in dispersion accompanied with number increase. Conclusion Reelin plays an important role in the development of neuronal migration, neural proliferation and cortical lamination, especially in the process of polarization of pyramidal cells.
Reelin / Cell differentiation / Cell polarization / Lamination / Immunofluorescence / Mouse
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