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Preparation and histological evaluation of the decellularized scaffold for porcine common bile duct
LI Wen-chun* LI Jing ZHANG Hong-mei WANG Han-qin
Acta Anatomica Sinica ›› 2015, Vol. 46 ›› Issue (5) : 694-698.
Preparation and histological evaluation of the decellularized scaffold for porcine common bile duct
Objective To evaluate the histological change of porcine common bile ducts before and after treatments with different acellular matrix, and to provide theoretical basis for the application of bile duct scaffold materials for tissue engineering. Methods Thirty porcine common bile ducts were randomly divided into 5 groups: control (A) group: 0.05% trypsin + nuclease (B), 0.1% sodium dodecyl sulphate (SDS) + nuclease(C), 1.0% triton X-100 + nuclease(D), 1.0% triton X-100 + 0.1% SDS + nuclease(E) groups. After HE staining, the tissue structure of acellular matrix and the residual cells were observed under a light microscope. The DNA content of acellular matrix was detected using UV spectrophotometry, and the acellular rate was calculated. Results Few residual cells and fiber damage were observed in group B. All the cells were removed and no obvious damages were done in groups C, D and E. The DNA content of group A was (71.24 ± 2.56)μg / 100 mg. The DNA contents of four acellular matrix treatment groups B, C, D and E were statistically different from that of group A (F=15.29, P<0.01). There was obvious acellular matrix treatment effect in the four groups (P< 0.01). The acellular rate of group B was 77.03%, the acellular matrix treatment effect of group B was slightly worse that of groups C, D and E (P< 0.05). The acellular rate of group E was up to 99.03%. Conclusion The acellular matrix treatment effect of 1.0% Triton X-100 + 0.1% SDS + nuclease is satisfactory. The immunogenicity of the common bile duct can be reduced, therefore, it is an ideal acellular method for porcine common bile ducts.
Common bile duct / Decellularization / Extracellular matrix / Scaffold-like material / HE staining / Pig
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