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Repair of spinal cord injury by using the tissue engineered nerve grafts constructed by silk fibroin/filament including seed cells
LIU Xiao-dong XUE Cheng-bin JU Qian-qian YANG Xiao-hua QIN Jian-bing TIAN Mei-ling Lü Guang-ming*
Acta Anatomica Sinica ›› 2015, Vol. 46 ›› Issue (5) : 596-601.
Repair of spinal cord injury by using the tissue engineered nerve grafts constructed by silk fibroin/filament including seed cells
Objective To explore a method for construction of tissue engineered nerve grafts (tissue-engineered nerve grafts, TENGs) using silk fibroin/filament in vitro and to evaluate their roles for repairing spinal cord injury. Methods Isolation and induction of rat skin derived precursors into Schwann cells (SCs). S-100 immunofluorescence histochemical staining was applied for the SCs identification. As seed cells, SCs differentiated from skin derived precursors (SKPs) cultured with silk fibroin conduit and silk filament scaffold in vitro. After culturing for 7 days, the TENGs were transplanted into the injury site of rat dorsal spinal cord (T8-T10) hemisection model. BBB score was evaluated at different time points after the operation. The spinal cord samples were obtained 8 weeks after the operation and immunofluorescence histochemical staining was applied to evaluate the regeneration of the injured spinal cord and the survival of seed cells. Results Under a phase contrast microscope, most of the seed cells were bipolar or tripolar. The immunofluorescence staining showed that SKP-SCs were S-100 positive. After the transplantation of the TENGs into spinal cord injury site, BBB scores were significantly higher in TENGs group than in control group from 4 weeks to 8 weeks. The GFP-positive seed cells were also investigated after 8 weeks after the operation. Conclusion The TENGs constructed by silk fibroin/filament including seed cells may promote repairing of rat spinal cord injury.
Central nervous system / Tissue engineering / Schwann cell / Silk fibroin / Spinal cord injury / Immunofluorescence / Rat
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