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Nicotinamide adenine dinucleotide kinase 2 is highly expressed in malignant brain tumors
GAO Wei DONG Shi-ye PAN Fei-yan LIU Dan-hui*
Acta Anatomica Sinica ›› 2015, Vol. 46 ›› Issue (5) : 587-590.
Nicotinamide adenine dinucleotide kinase 2 is highly expressed in malignant brain tumors
Objective To investigate the nicotinamide adenine dinucleotide kinase 2 (NADK2) expression level in malignant brain tumors.Methods Immunohistochemistry analysis was used to analyze the NADK2 expression level in 75 malignant brain tumors including 55 of astrocytoma, 13 of oligodendroglioma, 3 of medulloblastoma and 4 of ependymoma. The integrated absorbance (IA) was measured by ImageJ software. Results The immunohistochemistry results showed that 74/75 tumor samples showed strong NADK2 staining. The IA results suggested that there was no significant difference between different brain tumor types as well as grades. Conclusion The results indicate that high expression of NADK2 is a general characteristic of malignant brain tumors.
Nicotinamide adenine dinucleotide kinase 2 / Malignant brain tumors / Immunohistochemistry / Human
［1］Xu P, Sauve AA. Vitamin B3, the nicotinamide adenine dinucleotides and aging［J］. Mech Ageing Dev, 2010, 131(4):287-298.
［2］Heitzer T, Wenzel U, Hink U, et al. Increased NAD(P)H oxidasemediated superoxide production in renovascular hypertension: evidence for an involvement of protein kinase C［J］. Kidney Int, 1999, 55(1):252-260.
［3］Petrelli R, Felczak K, Cappellacci L. NMN/NaMN adenylyltransferase (NMNAT) and NAD kinase (NADK) inhibitors: chemistry and potential therapeutic applications［J］. Curr Med Chem,2011, 18(13):1973-1992.
［4］Zhang R. MNADK, a long-awaited human mitochondrion-localized NAD kinase［J］. J Cell Physiol, 2015,230(8):1697-1701.
［5］Zhang R. MNADK, a novel liver-enriched mitochondrion-localized NAD kinase［J］. Biol Open,2013, 2(4):432-438.
［6］Ohashi K, Kawai S, Murata K. Identification and characterization of a human mitochondrial NAD kinase［J］. Nat Commun, 2012, 3:1248.
［7］Kahn K, Finkel A. It is a tumor——current review of headache and brain tumor［J］. Curr Pain Headache Rep, 2014, 18(6):421.
［8］Woodworth GF, Dunn GP, Nance EA, et al. Emerging insights into barriers to effective brain tumor therapeutics［J］. Front Oncol, 2014, 4:126.
［9］Pollak N, Niere M, Ziegler M. NAD kinase levels control the NADPH concentration in human cells［J］. J Biol Chem, 2007, 282(46):33562-33571.
［10］Lerner F, Niere M, Ludwig A, et al. Structural and functional characterization of human NAD kinase［J］. Biochem Biophys Res Commun, 2001, 288(1):69-74.
［11］Houten SM, Denis S, Te Brinke H,et al. Mitochondrial NADP(H) deficiency due to a mutation in NADK2 causes dienoyl-CoA reductase deficiency with hyperlysinemia［J］. Hum Mol Genet, 2014, 23(18):5009-5016.
［12］Cea M, Cagnetta A, Fulciniti M, et al. Targeting NAD+ salvage pathway induces autophagy in multiple myeloma cells via mTORC1 and extracellular signal-regulated kinase (ERK1/2) inhibition［J］. Blood, 2012, 120(17):3519-3529.
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