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Effect of astragalus polysaccharide on histological structure, enzyme activity and Caspase-3 protein expression in the kidney of mice injured by ephedrine
WEI Ping-ting LI Chong-yang YU Shi-yuan* MENG Ru YUAN Jing
Acta Anatomica Sinica ›› 2015, Vol. 46 ›› Issue (4) : 564-571.
Effect of astragalus polysaccharide on histological structure, enzyme activity and Caspase-3 protein expression in the kidney of mice injured by ephedrine
Objective To investigate astragalus polysaccharide（APS）on histological structure and enzyme activity in kidney of mice injured by ephedrine. Methods Seventy-two Kunming mice were divided into 4 groups：the nature control group, the experimental control group,the APS1 and 2 groups. The experimental control group, and APS1 and 2 groups were intraperitoneally injected with 4.0 g/L of ephedrine, 0.2ml twice a day for 15 days. The APS1 and 2 groups were respectively intragastric ally administed with 0.3 ml APS (12.5 g/L,25g/L) 1 hour after ephedrine injection, the control group was injected and intragastric ally administed with the same amount of saline.The blood urea nitrogen (BUN) and creatinine(Cr) content of plasma as well as the activity of superoxide dismutase (SOD) and the malondialdehyde (MDA) content of kidney tissue in the kidney were determined by colorimetry. The kidney histological structures were observed by optical microscopy. The changes of Caspase-3 protein expression in kidney were detected by immunohistochemistry and Western blotting. Results The BUN and Cr content in the blood of the experimental control groups were higher than that of the nature control group. The SOD activity in kidney of the experimental control groups was lower than the nature control groups, while the content of MDA was higher than the nature control groups (P<0.05 or P< 0.01).The histological structure lesions of the experimental control group showed that the glomerular swelled seriously, the renal capsule lumen stenosis,the swelling and necrosis of the renal tubular epithelial cells accured in different degrees. The expressions of Caspase-3 protein in kidney of the experimental control groups were higher than the control groups (P<0.05 or P<0.01). The BUN and Cr content of plasma of the APS groups were lower than the experimental control groups. The SOD activity in the renal tissue of the APS groups increased,while the MDA content decreased (P<0.05 or P<0.01). The histological structure lesions of the APS groups were relieved, the renal capsule lumen were obvious, the swelling of the renal tubular reduced, the degeneration and exfoliating of the epithelial cells alleviated. The expression of Caspase-3 protein in kidney declined significantly (P<0.05 or P<0.01). Conclusion APS enhanced the cells antioxidant enzyme activity, and reduced the expression of Caspase-3 protein.Therefore, APS alleviated the damage of kidney tissue caused by ephedrine effectively, and significantly protected the mice form the ephedrine effects.
Astragalus polysaccharide / Ephedrine / Renal function / Histological structure / Superoxide dismutase / Colorimetric method / Western blotting / Mouse
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