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Generation of induced pluripotent stem cells derived from parthenogenetic embryonic stem cells and reprogramming of imprinted genes
SHAN Zhi-yan WU Bin ZHANG Yue XUE Yuan WU Yan-shuang SHEN Xing-hui LEI Lei LIU Zhong-hua*
Acta Anatomica Sinica ›› 2015, Vol. 46 ›› Issue (4) : 553-557.
Generation of induced pluripotent stem cells derived from parthenogenetic embryonic stem cells and reprogramming of imprinted genes
Objective To investigate the effect of pluripotency and the expression of imprinted genes on the parthenogenetic embryonic stem cells (PgESCs) by induced pluripotent stem cells (iPSCs) reprogrmming. Methods In our study, we firstly generated the PgESCs from parthenogenetic blastocyst, induced the pluripotent factors into the PgESCs by retrovirus transfection and generated the iPS cells derived from PgESCs. Results It showed no difference in pluripotency and differentiation between PgESCs and PgESC-iPS cells, however, the expression of maternal imprinted genes was significantly increased and the expression of paternal imprinted genes was decreased in PgESC-iPS cells compared to that in PgESCs. Conclusion These results demonstrated the expression of imprinted genes could be modified by iPSCs reprogramming, which may be close to the expression level of imprinted genes in fertilized embronic stem cells.
arthenogenetic stem cell / Induced pluripotent stem cell / Imprinted gene / Real-time PCR / Mouse
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