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Ultrastructure of lymphatic capillaries with tumor growth in Kunming mice transplanted with S180 sarcoma
MA Nan ZHANG Li-nan WANG Long-kai ZHAGN Xiao-bo BI Xiu GAO Zi-bin*
Acta Anatomica Sinica ›› 2015, Vol. 46 ›› Issue (4) : 503-508.
Ultrastructure of lymphatic capillaries with tumor growth in Kunming mice transplanted with S180 sarcoma
Objective To investigate and explore the ultrastructural change of lymphatic capillaries in Kunming mice transplanted with S180 sarcoma with tumor growth. Methods Fifteen Kunming mice were inoculated with S180 ascites via subcutaneous injection to establish the cancer model and divided into five groups. The ultrastructural features of lymphatic capillaries of S180 sarcoma transplanted in Kunming mice were observed by a transmission electron microscope (TEM) in order to analyze the ultrastructural change of lymphatic capillaries with tumor growth. Results The integral lymphatic capillary wall, the anchoring filament and collagen fiber, and the organelles with normal physiological structure were observed in 3 days group. The dissolved or damaged degree of the lymphatic capillary wall and organelles became more and more serious, and the number of the anchoring filament and collagen became less and less with tumor growth in 5 days group, 7 days group and 9 days group. The lymphatic capillary wall was completely destroyed or broken, and any anchoring filament and collagen fiber were scarcely observed, while the lymphatic capillary endothelial cells and the organelles were scarcely observed or identified in 11 day group. Conclusion With the development of transplanted S180, the tumor volume and weight were increased and the dissolved or damaged degree of the lymphatic capillary wall and the organelles became progressively worse.
S180 sarcoma / Lymphatic capillary / Ultrastructural feature / Transmission electron microscopy / Mouse
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