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Expression of nanog protein in fibroblasts from multiple organs of the neonatal rat
LU Zhao-hui CHANG Yu-qiao GUO Zhi-kun* ZHAO Guo-an LI Qiong LI Ci-xia
Acta Anatomica Sinica ›› 2015, Vol. 46 ›› Issue (4) : 488-494.
Expression of nanog protein in fibroblasts from multiple organs of the neonatal rat
Objective To investigate different expression and its significance of nanog protein in multiple organs fibroblasts of neonatal rats, including heart, liver, spleen, lung, kidney and skin. Methods The expresssion and co-expression of nanog and vimentin in fibroblasts were detected in multiple organs by immunohistochemistry and immunofluorescence. Fibroblasts in multiple organs cultured in vitrowere labeled with nanog and vimentin by immunofluorescence. Results Nanog and vimentin were detected in six organs in neonatal rats respectively. Co-expression rates of these two proteins were（10.07±0.77）% in heart,（9.97±3.70）% in liver,（11.23±2.98）% in spleen,（15.07±2.87）% in lung,（11.75±2.76）% in kidney and（16.14±1.28）% in skin. So co-expression rates of skin and lung were higher than that of other four organs（P<0.05）. Conclusion Fibroblasts in multiple organs which can express nanog may have stem cell-like characters and fibroblasts in skin and lung of neonatal rats have more potential for organ repair.
Nanog / Vimentin / Fibroblast / Immunohistochemistry / Immunofluorescence / Neonatal rat
［1］Chambers I, Colby D, Robertson M, et al. Functional expression cloning of Nanog, a pluripotency sustaining factor in embryonic stem cells［J］. Cell，2003，113(5):643-655.
［2］Chang LSh, Guo ZhK, Yan QJ, et al. The study progress of Nanog［J］. Medical Recapitulate, 2007, 13(17):1292-1294. (in Chinese)
常连生，郭志坤，严泉剑，等.“永生基因”Nanog的研究进展［J］. 医学综述，2007，13(17):1292-1294.
［3］Camelliti P，Green CR，LeGrice I，et al. Fibroblast network in rabbit sinoatrial node: structural and functional identification of homogeneous and heterogeneous cell coupling［J］. Circ Res，2004，94(6):828-835.
［4］Luo H, Li Q, Pramanik J, et al. Nanog expression in heart tissues induced by acute myocardial infarction［J］. Histol Histopathol，2014，29(10):1287-1293.
［5］Chang Y, Li H, Guo Z, et al. Mesenchymal stem cell-like properties in fibroblasts［J］. Cell Physiol Biochem，2014，34(3):703-714.
［6］Lorenz K, Sicker M, Schmelzer E, et al.Multilineage differentiation potential of human dermal skin derived fibroblasts［J］.Exp Dermato，2008，17(11):925-932.
［7］Darr H, Mayshar Y, Benvenisty N, et al. Overexpression of NANOG in human ES cells enables feeder-free growth while inducing primitive ectoderm features［J］. Development，2006，133(6):1193-1201.
［8］Kuijk EW, van Mil A, Brinkhof B, et al. PTEN and TRP53 independently suppress Nanog expression in spermatogonial stem cells［J］. Stem Cells Dev，2010，19(7):979-988.
［9］Zhang J, Wang X, Chen B, et al. Expression of Nanog gene promotes NIH3T3 cell proliferation［J］. Biochem Biophys Res Commun，2005，338(2): 1098-1102.
［10］You JS, Kang JK, Soe DW, et al. Deletion of embryonic stem cell signature by histone deacetylase inhibitor in NCCIT cells: involvement of Nanog suppression［J］. Cancer Res，2009，69(14): 5716-5725.
［11］Page RL, Ambady S, Holmes WF，et al. Induction of stem cell gene expression in adult human fibroblasts without transgenes［J］. Cloning Stem Cells，2009，11(3):417-426.
［12］Zhao XF, Wang DL, Wei ZR, et al. The research of fibroblasts from human hypertrophic scar showing a mesenchymal stem cell phenotype and multilineage differentiation potentialities［J］. Chinese Journal of Plastic Surgery, 2013, 29(4):273-278. (in Chinese)
赵小锋，王达利，魏在荣，等. 人增生性瘢痕成纤维细胞的间充质干细胞表型及多向分化潜能［J］. 中华整形外科杂志，2013，29(4):273-278.
［13］Hanifa MA，Collin MP，Buckley CD，et al. Mesenchymal stem cells：the fibroblasts’ new clothes ［J］ ? Haematologica，2009，94(2):258-263.
［14］Lin GL，Hankenson KD. Integration of BMP, Wnt, and notch signaling pathways in osteoblast differentiation［J］. Cell Biochem，2011，112(12):3491-3501.
［15］Junker JP，Sommar P，Skog M，et al. Chondrogenic and osteogenic diferentiation of clonally derived human demal fibroblasts［J］. Cells Tissues Organs，2010，191(2):105-118.
［16］Wu JF，Han JZh，Guo ZhK，et al. Expresssion of protein in the cardiac tissue of developing mouse［J］. Chinese Journal of Anatomy，2008，31(4)：496-544. (in Chinese)
武俊芳，韩金珠，郭志坤，等.Nanog蛋白在小鼠心肌组织发育中的表达［J］.解剖学杂志，2008，31(4)：496-544.
［17］Guo ZK，Guo K，Luo H，et al.The expression analysis of nanog gene in the developing rat myocardial tissues［J］. Cell Physiol Biochem，2015，35（3）:866-874.
［18］Taylor G, Lehrer MS, Jensen PJ, et al. Involvement of follicular stem cells in forming not only the follicle but also the epidermis［J］. Cell，2000，102(4):451-461.
［19］Ghadially R. Epidermal stem cells［J］. Adv Dermatol，2005，21: 335-355.
［20］Pozdnyakova O, Grossman J, Barbagallo B, et al. The hair follicle barrier to involvement by malignant melanoma［J］. Cancer，2009，115(6):1267-1275.
［21］Wang Z, Coleman DJ, Bajaj G, et al. RXR α ablation in epidermal keratinocytes enhances UVR-induced DNA damage, apoptosis, and proliferation of keratinocytes and melanocytes［J］. J Invest Dermatol， 2011，131(1):177-187.
［22］Lin F, Moran A, Igarashi P. Intrarenal cells, not bone marrow-derived cells, are the major source for regeneration in postischemic kidney［J］. J Clin Invest，2005，115(7):1756-1764.
［23］Lysy PA, Smets F, Sibille C, et al. Human skin fibroblasts: From mesodermal to hepatocyte-like differentiation［J］. Hepatology，2007，46(5):1574-1585.
［24］Carlson S, Trial J, Soeller C, et al. Cardiac mesenchymal stem cells contribute to scar formation after myocardial infarction［J］. Cardiovasc，2011，91(1):99-107.
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