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Effects of zinc on P53 protein expression in hippocampal neurons of mice with spinal nerve transection -induced neuropathic pain
WANG Yue-jing ZHANG Hai-yan LIAN Jie YAO Hong-bo SUN Li-hui* ZHANG Meng
Acta Anatomica Sinica ›› 2015, Vol. 46 ›› Issue (4) : 455-461.
Effects of zinc on P53 protein expression in hippocampal neurons of mice with spinal nerve transection -induced neuropathic pain
Objective To investigate the effects of the zinc on learning,memory and the expression of P53 in the hippocampus of mice with L5 spinal nerve transection -induced neuropathic pain(NPP). Methods Forty-eight adult male mice were randomly divided into 3 groups (n=16 per group): control group, NPP group and high zinc NPP group (zinc-NPP group). In the control group, the L5 spinal nerve was exposed but without ligation. In the NPP group, the nerve was ligated on the left. The mice of the zinc - NPP group drank a zinc sulfate solution after the left L5 spinal nerve ligation. After 29 days, the spatial learning and memory ability in three groups mice were measured by Morris water maze experiment. After the experiment was finished, cell apoptosis rate was detected by using flow cytometry，P53 expression of each group was tested by immunohistochemical and immune imprinting technology. Results The incubation period of finding the hidden platform was gradually shortened in the NPP group（21.03±12.08）,zinc-NPP group（16.97±8.59）and control group（8.74±3.61) (P<0.05). The proportion of the time and the path to the total time and the total path of finding the Hidden platform gradually increased in NPP group, Zn-NPP, and control group (P<0.05). Flow cytometry detection results shown that the apoptosis rate of Control group（1.88%），Zn-NPP group（16.12%）and NPP group（33.17%）gradually increase(P<0.05).The expression of P53 in the hippocampus CA1 area of the control group，Zn-NPP group and NPP group was 27.42±7.08, 37.52±10.58, and 53.31±9.26, respectively.The expression of P53 in hippocampus CA3 area of the control group，Zn-NPP group and NPP group was 29.51±8.31, 36.21±7.91, and 56.71±8.09, respectively. The expression of P53 in the hippocampus of the control group，Zn-NPP group and NPP group by immune imprinting technology was 29.51±8.31, 36.21±7.91, 56.71±8.09, respectively. All the result showed a gradually increasing trend. Conclusion Zinc can improve the ability of learning and memory in neuropathy pain mice.
Zinc / Neuropathy pain / Learning and memory / P53 / Immunohistochemistry / Hippocampus / Mouse
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