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Morphological study of Reelin regulation on the development of rostral migratory stream in the mouse
SHI Shu-qin CUI Zhan-jun YAN Ming-chao MU Xiao-yun DENG Jin-bo* YIN Ming-wei*
Acta Anatomica Sinica ›› 2015, Vol. 46 ›› Issue (4) : 433-442.
Morphological study of Reelin regulation on the development of rostral migratory stream in the mouse
Objective To investigate the differentiation of neural stem cells in the subventridular zone (SVZ) and neural migration along the the rostral migratory stream (RMS) into the olfactory bulb（OB), particularly, Reelin’s effect on the neural differentiation and migration in RMS. Methods Wild type mice (50 cases) and reeler mice (23 cases) from E16 (embryonic day 16) to P90 (postnatal day 90) were used for Nissl staining, immunofluorescent labeling, ink perfusion and ultrastructure observation to visualize neural stem cells, the radial glial cells and vasulature in neocortex and RMS. The development of RMS in two groups was compared. Results In embryonic days and early postnatal period, vascular niches in the SVZ consisted of neurons, glial cells, neural stem cells and vascular vessel which provided a ideal microenvironment for the differentiation and mturation of neural stem cells. After maturation in the vascular niche, the neural stem cells started to migrate tangentially along RMS toward olfactory bulb with the chain-guidable pattern along both glial and vascular scaffold. After entering in OB, the migrating pattern translated into the radial migration, and these cells differentiated into various neuron in olfactory bulb. In general, RMS’s development, neural stem cell differentiation and cell migration in reeler mice were similar to the wild type, howover, the distribution of vasculature in cortex and RMS was disordered, and the migrating cells often concentrated in olfactory bulb densely and scatteredly. Conclusion Niche in subventricular zone is the main sources of neural stem cells, and both vasculature and radial glia and acts as a scaffold for the migration of neural stem cells in RMS. As an important signal for cell migration, Reelin can affect the development of blood vessels and cell migration in RMS. Without Reelin, the cell migration in RMS will be blocked.
Rostral migratory stream / Reelin / Olfactory bulb / Vessel / Immunofluorescence / Mouse
［1］Reynolds BA, Weiss S. Generation of neurons and astrocytes from isolated cells of the adult mammalian central nervous system［J］. Science, 1992, 255(5052): 1707-1710.
［2］McKay R. Stem cells in the central nervous system［J］. Science, 1997, 276(5309): 66-71.
［3］Doetsch F. A niche for adult neural stem cells［J］. Curr Opin Genet Dev, 2003, 13(5): 543-550.
［4］Peretto P, Giachino C, Aimar P, et al. Chain formation and glialtube assembly in the shift from neonatal to adult subventricular zone of the rodent forebrain［J］. J Comp Neurol, 2005,487(4):407-427.
［5］Pais V, Danaila L, Pais E. The vascular stem cell niches and their significance in the brain［J］. J Neurosurg Sci, 2014, 58(3): 161-168.
［6］Palmer TD, Willhoite AR, Gage FH. Vascular niche for adult hippocampal neurogenesis［J］. J Comp Neurol, 2000, 425(4): 479-494.
［7］Xi Y, Xue Sh, Li LL, et al. Vsacular and stem-cellular niche and the neural proliferation and differentiation in spinal cord of the mouse［J］.Acta Anatomica Sinica, 2014, 45(2): 155-160. (in Chinese)
席艳, 薛帅, 李莉莉, 等. 小鼠脊髓血管-神经干细胞龛与神经细胞的增殖和分化［J］. 解剖学报, 2014, 45(2): 155-160.
［8］Silva-Vargas V, Crouch E, Doetsch F. Adult neural stem cells and their niche: a dynamic duo
during homeostasis, regeneration, and aging［J］. Curr Opin Neurobiol, 2013, 23(6): 935942.
［9］Sun Y, Jin K, Childs JT, et al. Vascular endothelial growth factorB (VEGFB) stimulates neurogenesis: evidence from knockout mice and growth factor administration［J］. Dev Biol, 2006, 289(2): 329-335.
［10］MartoncikovaM, Fabianova K, Schreiberova A, et al. Astrocytic and vascular scaffolding for neurolast migration in the rostral migratory stream［J］. Curr Neurovasc Res, 2014, 11(4): 321-329.
［11］Cheng WJ, Zhang WL, Li X, et al. Interaction between cell migration and vasculature in the developing cerebellum of mouse［J］. Acta Anatomica Sinica, 2013, 44(6): 740-747. (in Chinese)
陈文静, 张文玲, 李雪, 等. 小鼠小脑皮质发育过程中细胞迁移及血管之间的相互作用［J］. 解剖学报, 2013, 44(6): 740-747.
［12］Valero J, Weruaga E, Murias AR, et al. Changes in cell migration and survival in the olfactory bulb of the pcd/pcd mouse［J］. Dev Neurobiol, 2007, 67(7): 839-859.
［13］Jiang A, Hu W, Meng H, et al. Loss of VLDL receptor activates retinal vascular endothelial cells and promotes angiogenesis［J］. Invest Ophthalmol Vis Sci, 2009, 50(2): 844-850.
［14］Lakomá J, GarciaAlonso L, Luque J M. Reelin sets the pace of neocortical neurogenesis［J］. Development, 2011, 138(23): 5223-5234.
［15］Schwarz Q, Gu C, Fujisawa H, et al. Vascular endothelial growth factor controls neuronal migration and cooperates with Sema3A to pattern distinct compartments of the facial nerve［J］. Genes Dev, 2004, 18(22): 2822-2834.
［16］Howell KR, Hoda MN, Pillai A. VEGF activates NR2B phosphorylation through Dab1 pathway［J］. Neurosci Lett, 2013, 552: 30-34.
［17］Hack I, Bancila M, Loulier K, et al. Reelin is a detachment signal in tangential chainmigration during postnatal neurogenesis［J］. Nat Neurosci, 2002, 5(10): 939-945.
［18］Rice DS, Curran T. Role of the reelin signaling pathway in central nervous system development［J］. Annu Rev Neurosci, 2001, 24:1005-1039.
［19］Zhao S, Frotscher M. Go or stop? Divergent roles of Reelin in radial neuronal migration［J］. Neuroscientist, 2010, 16(4):421-434.
［20］Saghatelyan A, de Chevigny A, Schachner M, et al. TenascinR mediates activity-dependent recruitment of neuroblasts in the adult mouse forebrain［J］. Nat Neurosci, 2004, 7(4): 347-356.
［21］Ng KL, Li JD, Cheng MY, et al. Dependence of olfactory bulb neurogenesis on prokineticin 2 signaling［J］. Science, 2005, 308(5730): 1923-1927.
［22］Wichterle H, García-Verdugo JM, Alvarez-Buylla A. Direct evidence for homotypic, glia-independent neuronal migration［J］. Neuron, 1997, 18(5): 779-791.
［23］Derer P, Derer M, Goffinet A. Axonal secretion of Reelin by cajalretzius cells:evidence from comparison of normal and RelnOrl mutant mice［J］. J Comp Neurol, 2001, 440(2): 136-143.
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