Mesenteric marginal vessel arch attenuates intestinal ischemia-reperfusion injury by compensating blood-supply

doi:10.16098/j.issn.0529-1356.2015.03.018

Acta Anatomica Sinica ›› 2015 ›› Issue (3) : 394-399. DOI: 10.16098/j.issn.0529-1356.2015.03.018

WANG Qiang LI Xin-rui YANG Zheng* LI Chang-yin YI Fang YANG Wei

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Abstract

Objective To observe the effect of mesenteric marginal vessel arch attenuating intestinal ischemia-reperfusion（I/R）injury by compensating blood-supply for the intestinal canal in mice. MethodsNinety KM rats were randomly divided into normal group(A), sham-operation group(B), and clipping mesenteric artery group(C), clipping the two adjacent straight artery beside ileocecal valve group(D), and clipping the marginal vessel arch correspondence two adjacent straight artery near the pyloric end group(E) based on D group. The range and degree of ischemia-reperfusion in intestinal canal nourished by arteries were different because each group clipped different arteries. According to the different models in each group, the ileum propulsion rate, the frequency and amplitude of the slow wave and contractility were observed. HE staining was used to evaluate the intestinal mucosal injury by histological examination. Results The ileum propulsion rates of the model group(C,D,E group) were lower than that of the A group(P<0.01). Compared with the C group, the ileum propulsion rate of the D group was higher(P<0.05), and compared with E group, D group was higher(P<0.05). The frequency and amplitude of the slow wave of the model group were lower than that of the A group(P<0.01), while compared with the C and E groups, D group was higher(P<0.05). The frequency, amplitude and vitality of the contractility of the model group were lower than that of the A group (P<0.01), the results of D group in comparison with the C and E groups were similar. Compared to A group, the intestinal mucosal injury of the model group was more serious(P<0.01), and both the scores of C and E group were higher than D group(P<0.05). Conclusion Mesenteric marginal vessel arch can attenuate intestinal ischemia-reperfusion injury by compensating blood-supply for the intestinal canal.

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WANG Qiang LI Xin-rui YANG Zheng* LI Chang-yin YI Fang YANG Wei. Mesenteric marginal vessel arch attenuates intestinal ischemia-reperfusion injury by compensating blood-supply[J]. Acta Anatomica Sinica. 2015(3): 394-399 https://doi.org/10.16098/j.issn.0529-1356.2015.03.018

References

［1］Wang ShA, Zhong ZhY, Wang JT, et al. Morphological observation of ileum straight arteries and organs surface artery［J］. Heilongjiang Medicine and Pharmacy, 2003, 26(4):105-106.(in Chinese)
王守安,钟震亚,王景涛,等. 回肠直动脉及器官表面动脉的形态观察［J］.黑龙江医药科学,2003,26(4):105-106.
［2］Yamamoto M, Plessow B, Koch HK. Electron microscopic studies on the small intestinal mucosa of rats after mechanical intestinal obstruction and ischemia［J］. Virchow Arch B Cell Pathol, 1980, 32(2):157-164.
［3］Chui CJ, Mcardle AH, Browm R, et al. Intestinal mucosal lesion in low-flow states［J］. Arch Surg, 1970, 101:478-483.
［4］Gulati P, Singh N. Evolving possible link between P13K and NO pathways in neuroprotective mechanism of ischemic postconditioning in mice［J］. Mol Cell Biochem, 2014, 397(1-2):255-265.
［5］Seidl H, Gundling F, Pehl C, et al. Multiple intraluminal impedance measurement and manometry in the jejunum of healthy subjects: a new tool for diagnosis of small bowel motility［J］. Digestion, 2010, 81(3):172-180.
［6］Nikitenko VI, Stadnikov AA, Kopylov VA. Bacterial translocation from the gastrointestinal tract in healthy and injured rats［J］. J Wound Care, 2011, 20(3):114-122.
［7］Xing D, Zheng R, Li S, et al. Pivotal role of mast cell carboxypeptidase A in mediating protection against small intestinal ischemia-reperfusion injury in rats after ischemic preconditioning［J］. Journal Surg Res, 2014, 192(1):177-186.
［8］Gao LY. Anatomical study of the marginal arterial arch of colon［J］. Acta Anatomica Sinica, 1980, 11(1):23-27.(in Chinese)
高龙远.国人结肠边缘动脉弓的解剖学研究［J］. 解剖学报, 1980, 11(1):23-27.
［9］Wang ShA, Zhang ShCh, Tian GZh, et al. The microvascular architecture of ileum in adults and fetus［J］. Progress of Anatomical Sciences, 2010, 16(5):463-466.(in Chinese)
王守安，张胜昌,田国忠,等.成人和胎儿回肠器官内微血管的构筑［J］.解剖科学进展,2010,16(5):463-466.
［10］Leite Junior R, Mello NB, Pereira Lde P, et al. Enterocyte ultrastructural alterations following intestinal obstruction in rats［J］. Acta Cir Bras, 2010, 25(1):2-8.
［11］Li GX, Ding ZH, Zhang C, et al. Inferior mesenteric vessels related clinical anatomy in Iaparoscopy［J］. Chinese Journal of Anatomy, 2006, 29(5):624-626.(in Chinese)
李国新,丁自海,张策,等.腹腔镜下肠系膜下血管的临床解剖学［J］.解剖学杂志,2006,29(5):624-626.
［12］Liao D, Zhao J,Gregersen H. 3d Mechanical properties of the partially obstructed guinea pig small intestine［J］. J Biomech, 2010, 43(11):2079-2086.
［13］Zhu WM, Gu LL. Prevention and management of intestinal complications after mesenteric occlusive disease surgery［J］. Chinese Journal of Practical Surgery, 2013, 33(12): 1025-1027.(in Chinese)
朱维铭, 顾立立. 肠系膜血管疾病术后肠道并发症防治［J］.中国实用外科杂志,2013,33(12):1025-1027.
［14］Gao JL, Yang LQ, Han JX. Discussion on the method of terminal ileum anastomosis［J］. Shanxi Medical Journal, 2013, 42(5):558-559.(in Chinese)
高继良,杨利群,韩建勋.末段回肠端端吻合方法探讨［J］.山西医药杂志,2013,42(5):558-559.