Protective effects of Shandan Sphallerocarpus gracilispolysaccharide on chronic hepatic injury in mice induced by CCl4

doi:10.16098/j.issn.0529-1356.2015.03.017

Acta Anatomica Sinica ›› 2015 ›› Issue (3) : 387-393. DOI: 10.16098/j.issn.0529-1356.2015.03.017

Protective effects of Shandan Sphallerocarpus gracilispolysaccharide on chronic hepatic injury in mice induced by CCl₄

YUAN Jing¹YU Shi-yuan^1,2* MENG Ru¹WEI Ping-ting¹

Author information +

History +

Abstract

Objective To investigate the effect of Shandan Sphallerocarpus gracilispolysaccharide(SGP) on the hepatic injury in mice induced by carbon tetrachloride(CCl₄).
Methods Forty mice were divided into 5 groups: the nature control group，the experimental control group，and 3 SGP groups. The mice in SGP groups were administered with SGP at the doses of 12.5，25，37.5 g/L 0.2ml，respectively, the other groups were gavaged with 0.2ml saline, the nature control group was infected by 0.2ml injected by 0.2ml saline. The morphological changes for hepatic tissues were observed under microscopy，the activities of plasma alanine aminotransferase (ALT)，aspartate aminotransferase (AST)，alkaline phosphatase(ALP) and superoxide dismutase(SOD)，as well as content of malondialdehyde(MDA) in liver were determined by colorimetry，the expression change of Caspase-3 and Bax in liver tissue was determined by the immunohistochemical method.
Results The activities of plasma ALT，AST，ALP was significantly increased in the experimental control group，compared with the nature control group（P<0.01），while the activity of SOD in liver tissue decreased obviously（P<0.01），and the contents of MDA were increased significantly（P<0.01），the livers tissue structure in experimental control group was blur，liver cells were necrosis and had serious vacuolation. The activities for Caspase-3 and Bax immunohistochemical positive staining in hepatic tissues significantly increased in the experimental control group (P<0.01).However，the activities of plasma ALT，AST and ALP in the SGP groups was significantly decreased（P<0.01），the activitys of SOD in liver tissue were increased significantly（P<0.01），and the contents of MDA were significantly decreased（P<0.01），the liver structures were normal， inflammatory necrosis were decreased， liver celluar stuctures were clear，the positive expression of Caspase-3 and Bax protein were decreased significantly，compared with the experimental control group（P<0.01）Conclusion The SGP has a good effect on improving the cellular antioxidant activity，exhibiting activities of anti-oxidant and anti-inflammatory, and protecting the liver from the damage induced by carbon tetrachloride in mice, and has a dose effect within a certain range.

Cite this article

EndNote

Ris (Procite)

Bibtex

Download Citations

YUAN Jing YU Shi-yuan* MENG Ru WEI Ping-ting. Protective effects of Shandan Sphallerocarpus gracilispolysaccharide on chronic hepatic injury in mice induced by CCl₄[J]. Acta Anatomica Sinica. 2015(3): 387-393 https://doi.org/10.16098/j.issn.0529-1356.2015.03.017

References

［1］Guo Y，Tian XZh，Zhang BL，et al. Characteristics of carbon tetrachloride pollution in groundwater in a water source［J］.South-to-north Water Diversion and Water Science and Technology，2011，3（9）：109-113.(in Chinese)
郭英，田西昭，张立宝，等.某水源地地下水四氯化碳污染特征分析［J］.南水北调与水利科技，2011，3（9）：109-113.
［2］Han BP，Wang XY，Zhu XQ，et al.Groundwater contamination by carbon tetrachlor in karstic area in China［J］.Acta Scientiae Circumstantiae，2004，24（6）：983-988.(in Chinese)
韩宝平，王小英，朱雪强，等.某市岩溶水源地四氯化碳污染特征研究［J］.环境科学学报，2004，24（6）：983-988.
［3］Xue HY.Analysis of Shandan Sphallerocarpus Gracilis chemical composition and biological activity research［D］. Lanzhou University of Technology，2011:2-6. (in Chinese)
薛鸿雁.山丹黄参化学成分分析及生物学活性研究［D］ . 兰州理工大学，2011:2-6.
［4］Jia L，Nie XJ，Fang M，et al.Effect of Sphallerocarpus Gracilis polysaccharides on muscular free radical metabolism and ultrastructure in mice［J］.Journal of Hebei Institute of Physical Education，2010，24(5)：58-62.(in Chinese)
贾磊，聂秀娟，方梅，等.黄参多糖对小鼠骨骼肌自由基代谢及超微结构的影响［J］.河北体育学院学报，2010，24(5)：58-62.
［5］Jia L，Nie XJ，Xiao W，et al. Experimental study into intervenient effects of Sphallerocarpus Gracilis polysacchorides on immunity in trained mice［J］.Journal of Chengdu Institute of Physical Education，2010，36(7)：72-76.(in Chinese)
贾磊，聂秀娟，肖雯，等.黄参多糖干预运动小鼠免疫功能的实验研究［J］.成都体育学院学报，2010，36(7)：72-76.
［6］Luo GH，Li JY，Ye ShB，et al.Optimization of process parameters for the extraction of polysaccharides from Sphallerocarpus gracilis roots［J］.Food Science，2013，34（24）：40-45.(in Chinese)
罗光宏，李金燕，叶生宝，等.黄参多糖提取工艺参数优化［J］.食品科学，2013，34（24）：40-45.
［7］Lu SP，Zhao PT.Chemical characterization of Lycimn Barbarum polysaccharides and their reducing myocardial injury in ischemia/reperfusion of rat heart［J］.International Journal of Biological Macromolecules，2010，(47)：681-684.
［8］Li ChY，Wang JJ，Yu ShY.Effect of Chinese traditional medicine on activities of antioxidant enzyme and special protein of kidney in ephedrine addiction mice［J］.Acta Anatomica Sinica，2013，3(44)：372-378.(in Chinese)
李重阳，王锦锦，俞诗源.中药复方对麻黄素成瘾小鼠肾抗氧化酶和特异蛋白的影响［J］.解剖学报，2013，3(44)：372-378.
［9］Li L，Yu ShY，Ma JB，et al.Effects of melamine on histological structure and activities of superoxide dismutase，catalase and content of maleic dialdehyde in kidney of filial mice［J］.Acta Anatomica Sinica，2011，42（2）：91-98.(in Chinese)
李丽，俞诗源，马建博，等.三聚氰胺对仔鼠肾脏组织结构和超氧化物歧化酶、过氧化氢酶活性及丙二醛含量的影响［J］ .解剖学报，2011，42（2）：91-98.
［10］Yao HZh，Li JX，Zheng HN.Effects of Isodon rubsecens extact on CCl4-induced chronic hepatic injury in mice ［J］.Lishizhen Medicine and Material Medica Research，2010，21(3)：575-577.(in Chinese)
姚会枝，李吉学，郑海娜.冬凌草提取物对四氯化碳诱导的小鼠慢性肝损伤的保护作用［J］.时珍国医国药，2010，21(3)：575-577.
［11］Gu YF，Li WM，Li ShH，et al.Experimental study on protective effect of Ziziphus Jujubae polysacchaside on liver injury caused by CCl4 ［J］.Chinese Journal of Traditional Medical Science and Technology，2006，13(2)：105-107.(in Chinese)
顾有芳，李卫民，李升和，等.大枣多糖对四氯化碳诱发肝损伤防护作用的实验研究［J］.中国中医药科技，2006，13(2)：105-107.
［12］Wang YZh.The protective effect of polysaccharide of Grifola Frondosa on carbon tetrachloride-induced liver injury and its mechanism［D］.Ji’nan：Shandong University，2010: 39-41.(in Chinese)
王玉卓.灰树花多糖对四氯化碳肝损伤的保护作用及其机制的研究［D］.济南：山东大学，2010:39-41.
［13］Zhou R，Tian ChR，Gao ChY，et al.Study on antioxidant activity of different solvents extracts of Sphallerocarpus gracilis［J］.Food Research and Development，2012，33（8）：58.(in Chinese)
周瑞，田呈瑞，高春燕，等.黄参不同溶剂提取物抗氧化活性研究［J］.食品研究与开发，2012，33（8）：58.
［14］Guo J，Zhang J，Wang JL，et al.Protection effects of Sphallerocarpus Gracilis polysaccharides on islet cell induced by alloxan ［J］.Gansu Science and Technology，2013，29（7）：124-126.(in Chinese)
郭杰，张继，王俊龙，等.黄参多糖对四氧嘧啶损伤胰岛细胞的保护作用［J］.甘肃科技，2013，29（7）：124-126.
［15］Sun J，Li ChY，Yu ShY，et al.Effects of ephedrine on structure and polypeptides in the liver of filial mice［J］.Acta Anatomica Sinica，2012，43(3)：398-404.(in Chinese)
孙隽，李重阳，俞诗源，等.麻黄素对仔鼠肝脏组织结构和相关活性物质的影响［J］.解剖学报，2012，43(3)：398-404.
［16］Jia L，Nie XJ，Fang M，et al. Review about influences of Sphallerocarpus Gracilis polysaccharides on serum CK, LDH, muscle glycogen, hepatic glycogen and sport endurance in mice［J］.Journal of Jiling Institute of Physical Education，2010，26(4)：4-6.(in Chinese)
贾磊，聂秀娟，方梅，等.黄参多糖对小鼠血清CK、LDH和MG、HG及运动耐力影响的研究［J］.吉林体育学院学报，2010，26(4)：4-6.
［17］Braunersreuther V，Viviani GL，Mach F，et al.Role of cytokes and chemokines in non-alcoholic fatty liver disease ［J］.World J Gastroenterol，2012，18(8)：727-735.
［18］Li JT，Wang JL，Fu ShG.The activity changes of alkaline phosphatase and its ultrastructual localization during liver regeneration rats ［J］.Acta Anatomica Sinica，2004，35(4)：392-395.(in Chinese)
李金亭，王俊丽，傅山岗.大鼠肝再生过程中碱性磷酸酶活性变化及其超微细胞化学研究［J］.解剖学报，2004，35 (4)：392-395.
［19］Sato R.Stero metabolism and SREBP activation ［J］.Arch Biochem Biophys，2010，501(2)：177-181.
［20］Aram G，Potter JJ，Liu X，et al. Deficiency of nicotinamide adenine dinucleotide phosphate，reduced form oxidase enhances hepatocellular injury but attenuates fibrosis after chronic carbon tetrachloride administration［J］.Hepatology，2009，49（3）：911-919.
［21］Frances D，Ronco MT，Ochoa E，et al.Oxidative stress in primary culture hepatocytes isolated from partially hepatectomized rats［J］.Can J Physiol Pharmacol,2007,85（10）：1047-1051.
［22］Bai JY，Lei PY，Wang YP，et al.Effects of carbon tetrachloride on triglyceride content in HL-7702 liver cells［J］.Chinese Journal of Public Health，2014，30（1）：86-88.(in Chinese)
白剑英，雷佩玉，王幼萍，等.四氯化碳对HL-7702肝细胞甘油三酯含量的影响［J］.中国公共卫生，2014，30（1）：86-88.
［23］Chen WY.Effect and mechanism of Fructus schisandrae on CCl4-induced hepatic injury liver cell repair in mice［D］.Shantou：Shantou university，2003:30-55.(in Chinese)
陈玮莹.五味子对CCl4肝损伤大鼠肝细胞修复及其机制［D］.汕头大学，2003:30-55.
［24］Tian L，Liu H，Xie YH .Inflammatory stress exacerbates cholesterol import in fatty liver of CCl4-induced mice via LDLr-SREBP-2-SCAP pathway ［J］.Journal of Third Military Medical University，2014，36（11）：1158-1162.(in Chinese)
田琳，刘宏，谢友红.炎症通过SCAP-SREBP2-LDLr途径促进四氯化碳诱导的小鼠脂肪变肝细胞胆固醇内流［J］.第三军医大学学报，2014，36（11）：1158-1162.
［25］Yan Zh，Cynthia G，Andren LB.Selective and protracted apoptosis in human primary neurons microinjection with active Caspase-3，-6，-7 and-8［J］.J Neurosci，2000，20(22)：8384-8389.
［26］Yu ShY，Meng R，Li ChY，et al.The effect of Angelica sinensis polysaccharide on activities of antioxidant enzyme and expression of NF-kB,TNF-α in liver tissue of the mice injured by ephedrine ［J］.Journal of Northwest Normal University，2015，51（1）:75-81.(in Chinese)
俞诗源，孟茹，李重阳，等.当归多糖对麻黄素小鼠肝组织抗氧化酶活性和NF-kB、TNF-α表达的影响［J］.西北师范大学学报，2015，51（1）:75-81.