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Effects of dihydromyricetin on apoptosis of lung adenocarcinoma A549 cells
SUN Da-yong ZUO Yan-zhen LIANG Hong-hu SONG Ji* MA Chun-hu SUN Hao-yang
Acta Anatomica Sinica ›› 2015 ›› Issue (3) : 359-362.
Effects of dihydromyricetin on apoptosis of lung adenocarcinoma A549 cells
Objective To study the effect of dihydromyricetin on apoptosis of hunman nonsmall cell lung cancer A549 cells, and to explore new drugs for lung cancer.
Methods A549 cells were treated with various concentrations of dihydromyricetin for 48hours. MTT assay was used to determined the proliferation ability of A549 cells, flow cytometry was used to detect the influence of dihydromyricetin on the cell apoptosis of A549 cells, Western blotting analysis was used to evaluate the levels of Bax, Bcl-2 expressions. Results MTT assay results showed a significant inhibition in A549 cells with a dose-dependent manner, when compared with control cells, the inhibitory concentration for 50%（IC50）was 64.45mg/L at 48 hours; Flow cytometry showed that dihydromyricetin induced apoptosis of A549 cells in a dose-dependent manner as well. Further analysis by Western blotting showed that the expression of Bax was increased, while the expression of Bcl-2 was reduced when A549 cells was incubated for 48 hours with dihydromyricetin. Conclusion Dihydromyricetin plays a role of anti-tumor through inhibiting A549 cells proliferation and inducing apoptosis.
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