Change of apoptosis factor expression in cochlear nucleus in carboplatin-induced auditory neuropathy rat

LIU Jing LI Zheng-li HE Qing HUANG Hong-yan*

Acta Anatomica Sinica ›› 2015 ›› Issue (3) : 324-328.

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Acta Anatomica Sinica ›› 2015 ›› Issue (3) : 324-328. DOI: 10.16098/j.issn.0529-1356.2015.03.006

Change of apoptosis factor expression in cochlear nucleus in carboplatin-induced auditory neuropathy rat

  • LIU Jing1 LI Zheng-li2 HE Qing3 HUANG Hong-yan 1*
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Abstract

Objective To investigate the influence of carboplatin (CBP) on cell apoptosis in the cochlear nucleus and the nerve protection of dexamethasone (DEX) in rats. Methods CBP was used to establish the animal model of auditory neuropathy (AN) through an intraperitoneal injection. Rats were assigned to NS group, CBP group and DEX+CBP group, 18 rats per group. On day 3 and day 6 post modeling, the immunoreactivity of Caspase-3 in the cochlear nucleus was detected by immunohistochemistry and the apoptotic protease activating facter 1 (Apaf-1) mRNA expression was explored by RT-PCR. Results The level of immunoreactivity of Caspase-3 and expression of Apaf-1 mRNA in the ventral cochlear nucleus(VCN) and dorsal cochlear nucleus (DCN) of CBP group were significantly higher than those in NS group and DEX+CBP group (P<0.05), while the levels between NS group and DEX+CBP group had no significant differences(P>0.05), and the effect on day 6 was more obvious than that on day 3. Conclusion CBP can not only damage the auditory nerve but also cause cell apoptosis on the cochlear nucleus in rats, and DEX weakens the effect of apoptosis for its neuroprotection.

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LIU Jing LI Zheng-li HE Qing HUANG Hong-yan*. Change of apoptosis factor expression in cochlear nucleus in carboplatin-induced auditory neuropathy rat[J]. Acta Anatomica Sinica. 2015(3): 324-328 https://doi.org/10.16098/j.issn.0529-1356.2015.03.006

References

[1]Yuan Y, Shi F, Yin Y, et al. Ouabaininduced cochlear nerve degeneration: synaptic loss and plasticity in a mouse model of auditory neuropathy[J]. J Assoc Res Otolaryngol, 2014, 15(1): 31-43. 
[2]Cowper-Smith CD, Dingle RN, Guo Y, et al. Synchronous auditory nerve activity in the carboplatin-chinchilla model of auditory neuropathy[J]. J Acoust Soc Am, 2010, 128(1): 56-62.
[3]Gu R. What is auditory neuropathy[J]. Chinese Journal of Otorhinolaryngology, 2002,37(4): 5-6. (in Chinese)
顾瑞. 什么是听神经病[J]. 中华耳鼻咽喉科杂志, 2002,37(4): 5-6.
[4]Sinha SK, Barman A, Singh NK, et al. Vestibular test findings in individuals with auditory neuropathy: review[J]. J Larynqol Otol, 2013, 127(5): 448-451.
[5]Ding DL, Qi WD, Qu Y, et al. Carboplatin and its ototoxicity[J]. Chinese Journal of Otology, 2008,6(2): 134-144.(in Chinese)
丁大连, 亓卫东, 屈燕, 等. 卡铂及其耳毒性[J]. 中华耳科学杂志, 2008,6(2): 134-144.  
[6]Ni DF. The current situation of auditory neuropathy clinic research[J]. Journal of Audiology and Speech Pathology, 2005, 13(4): 226-229.(in Chinese)
倪道凤. 听神经病临床研究现状[J]. 听力学及言语疾病杂志, 2005, 13(4): 226-229.
[7]Paxinos G, Watson C, Zhuge QCh. The Rat Brain in Stereotaxic Coordinates[M]. Beijing: People’s Medical Publishing House, 2005:53-64.(in Chinese)
George Paxinos, Charles Watson, 诸葛启钏. 大鼠脑立体解剖图谱[M]. 北京: 人民卫生出版社, 2005:53-64.
[8]Kraus KS, Ding D, Zhou Y, et al. Central auditory plasticity after carboplatin-induced unilateral inner ear damage in the chinchilla: up-regulation of GAP-43 in the ventral cochlear nucleus[J]. Hear Res, 2009, 255(1-2): 33-43.
[9]Schmiedt RA, Okamura HO, Lang H, et al. Ouabain application to the round window of the gerbil cochlea: a model of auditory neuropathy and apoptosis[J]. J Assoc Res Otolaryngol, 2002, 3(3): 223-233.
[10]Gilels F, Paquette ST, Zhang J, et al. Mutation of Foxo3 causes adult onset auditory neuropathy and alters cochlear synapse architecture in mice[J]. J Neurosci, 2013, 33(47): 18409-18424.
[11]Li C, Shi H, Ye H, et al. Minocycline cannot protect neurons against bilirubin-induced hyperexcitation in the ventral cochlear nucleus[J]. Exp Neurol, 2012, 237(1): 96-102.
[12]Zhu ChG, Li YQ, Gu XS, et al. Neuroanatomy[M]. Beijing: People’s Medical Publishing House, 2009: 340-345.(in Chinese)
朱长庚, 李云庆, 顾晓松,等. 神经解剖学[M]. 北京: 人民卫生出版社, 2009: 340-345.
[13]Erdur O, Kayhan FT, Cirik AA. Effectiveness of intratympanic dexamethasone for refractory sudden sensorineural hearing loss [J]. Eur Arch Otorhinolaryngol, 2014, 271(6): 1431-1436.
[14]Herr I, Gassler N, Friess H, et al. Regulation of differential pro-and anti-apoptotic signaling by glucocorticoids[J]. Apoptosis, 2007, 12(2):271-291. 
[15]Wang AZh, Zhang ZhY, Zhang YM, et al. Influence of dexamethasone on cellular inhibitor of apoptosis protein 1 gene expression and Caspase-3 activity in brain after cerebral hypoxia-ischemia[J]. Journal of Applied Clinied Pediatrics, 2007, 22(12): 923-924.(in Chinese)
王爱珍, 张振宇, 张元明, 等. 地塞米松对脑缺氧缺血新生大鼠细胞凋亡抑制蛋白1mRNA及Caspase-3活性的影响[J]. 实用儿科临床杂志, 2007, 22(12): 923-924.
[16]Yan JH, Wang ZhY, Liu XX, et al. The expression of NF-κB in spinal ganglia of the asthmatic guinea pigs and the inhibitory effect of dexamethsone[J]. Acta Anatomica Sinica, 2005,36(1):14-18.(in Chinese)
闫军浩, 王振宇, 刘晓湘, 等. NF-κB在哮喘豚鼠脊神经节的表达及地塞米松的抑制作用[J]. 解剖学报, 2005,36(1):14-18.
[17]Shin YH, Chung H, Park C, et al. Adenosine 5’-triphosphate (ATP) inhibits schwann cell demyelination during wallerian degeneration [J]. Cell Mol Neurobiol, 2014, 34(3): 361-368.

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