Welcome to visit Acta Anatomica Sinica! Today is
Chinese
Protective effect of hydrogen sulfide on brain damage in rats with subarachnoid hemorrhage
LIU Yan-mei ZHU Qian LINGHU Yan WANG Jian-qiang LIU Jun LI Wen-jie WANG Min-chen WU Kai-yun*
Acta Anatomica Sinica ›› 2015 ›› Issue (3) : 297-303.
Protective effect of hydrogen sulfide on brain damage in rats with subarachnoid hemorrhage
Objective To explore the effect of hydrogen sulfide (H2S) to the brain injury in rats with subarachnoid hemorrhage (SAH). Method Thirty experimental rats were randomly divided into Control group (n=10), SAH model group (n=10) and NaHS (100 mg/Kg)group (n=10),The experimental rats of the H2S group were injected NaHS 100 mg /Kg body weight in intraperitoneal and after surviving for 24 hours to do the neurological assessment and take the whole brain. The expression changes of GFAP, S-100b, Bcl-2, C-fos, caspase -3, MMP-9 were detected with fluorescent immunocytochemistry and Western blot method. Results Neurological assessment showed that severe neurological symptoms was significantly relieved in hydrogen sulfide group. GFAP and S-100b have a high expression in SAH rats group and were significantly decreased in H2S pretreatment group. The expression of Bcl-2 and caspase -3 in SAH group were high than in the control group but the expression of Bcl-2 was increased in H2S pretreatment group than SAH group, and caspase -3 was decreased in H2S pretreatment group. Western blot analysis showed that the expression of c-fos and MMP-9 were increased in SAH group and was significantly decreased in H2S pretreatment group. Conclusion The hydrogen sulfide have an significant protective effect on brain injury after SAH and its mechanism may be improved the function of glial cells and apoptotic pathways.
［1］Heuer GG, Smith MJ, Elliott JP, et al. Relationship between intracranial pressure and other clinical variables in patients with aneurysmal subarachnoid hemorrhage ［J］. J Neurosurg, 2004,101(3)408-416.
［2］Macdonald RL, Kassell NF, Mayer S, et al. Clazosentan to overcome neurological ischemia and infarction occurring after subarachnoid hemorrhage (CONSCIOUS-1): randomized, double-blind, placebo-controlled phase 2 dose-finding trial ［J］. Stroke, 2008, 39(11): 3015-3021.
［3］Pluta RM, Hansen-Schwartz J, Dreier J, et al. Cerebral vasospasm following subarachnoid hemorrhage: time for a new world of thought ［J］. Neurol Res, 2009, 31(2): 151-158.
［4］Yan JH, Khatibi NH, Han HB, et al. p53-Induced uncoupling expression of aquaporin-4 and inwardly rectifying K+4.1 channels in cytotoxic edema after subarachnoid hemorrhage ［J］. CNS Neurosci Ther, 2012,18(4):334-342.
［5］Yan JH，Zhou ChM. The roles of p53 in the early brain injury and vasospasm after subarachnoid hemorrhage ［J］. Progress of Anatomical Sciences, 2009,15(2):227-232.(in Chinese)
闫军浩，周长满.P53蛋白在蛛网膜下腔出血后早期脑损伤和血管痉挛中的作用 ［J］. 解剖科学进展，2009,15(2):227-232.
［6］Ayer R, Zhang J. Connecting the early brain injury of aneurismal subarachnoid hemorrhage to clinical practice ［J］. Turk Neurosurg, 2010, 20(2): 159-166.
［7］Wei X, Duan L, Bai L, et al. Effects of exogenous hydrogen sulfide on brain metabolism and early neurological function in rabbits after cardiac arrest ［J］. Intensive Care Med, 2012, 38(11): 1877-1885.
［8］Campolo M, Esposito E, Ahmad A, et al. A hydrogen sulfide-releasing cyclooxygenase inhibitor markedly accelerates recovery from experimental spinal cord injury ［J］. FASEB J, 2013, 27(11): 4489-4499.
［9］Sugawara T, Ayer R, Jadhav V, et al. A new grading system evaluating bleeding scale in filament perforation subarachnoid hemorrhage rat model ［J］. J Neurosci Methods, 2008, 167(2): 327-334.
［10］Bederson JB, Pitts LH, Tsuji M, et al. Rat middle cerebral artery occlusion: evaluation of the model and development of a neurologic examination ［J］. Stroke, 1986, 17(3): 472-476.
［11］Chen Y, Swanson RA. Astrocytes and brain injury ［J］. J Cereb Blood Flow Metab, 2003, 23(2): 137-149.
［12］Takuma K, Baba A, Matsuda T. Astrocyte apoptosis: implications for neuroprotection ［J］. Prog Neurobiol, 2004, 72(2): 111-127.
［13］Li YH, Yan P. Research progress of the expression of GFAP and NGF after brain injury ［J］. Chinese Journal of Forensic Medicine, 2003,18(6): 378-380. (in Chinese)
李玉红, 闫平.脑损伤后GFAP 和 NGF的表达研究进展［J］. 中国法医学杂志, 2003,18(6): 378-380.
［14］Wu X,Wang DW, Zhang GH, et al. The changes of GFAP, PCNA after brain contusion: an immunohistochemical study in forensic pathology ［J］. Journal of Forensic Medicine, 1999, 15 (2): 65-67. (in Chinese)
吴旭, 汪德文, 张国华,等.实验性脑挫伤 GFAP, PCNA 免疫组织化学研究 ［J］. 法医学杂志, 1999, 15 (2): 65-67.
［15］Weiss N, Sanchez-pena P, Roche S, et al. Prognosis value of plasma S100B protein levels after subarachnoid aneurismal hemorrhage ［J］. Anesthesiology, 2006, 104(4): 658-666.
［16］Wang YY. Progress of neurochemical marker S100B protein ［J］. Journal of Shanxi Medical University, 2006, 37(4): 437-439. (in Chinese)
王艳艳. 神经生化标志物S100B蛋白研究进展 ［J］. 山西医科大学学报, 2006, 37(4): 437-439.
［17］Luo ChX, Yi B, Chen YJ, et al. Effect of glial activation on early brain injury after subarachnoid hemorrhage in rats［J］. Medical Journal of Chinese People’s Liberation Army,2011, 36(12): 1348-1350. (in Chinese)
罗春霞, 易斌, 陈渝杰, 等. 胶质细胞活化在大鼠蛛网膜下腔出血后早期脑损伤中的作用 ［J］. 解放军医学杂志, 2011, 36(12): 1348-1350.
［18］Shimizu S, Nagayama T, Jin KL, et al. Bcl-2 Antsence treatment prevents induction of tolerance to focal ischemia in the rat brain ［J］. J Cereb Blood Flow Metab, 2001, 21(3): 233-243［19］Wang X, Gao XD, Gao B, et al. Cerebral lymphatic blockage aggravates apoptosis of cortical neurons after subarachnoid hemorrhage in rats in vivo ［J］. Acta Physiologica Sinica, 2010, 62 (2): 109-114. (in Chinese)
王轩，高向东，高兵，等. 脑淋巴引流阻滞加重蛛网膜下腔出血后大鼠皮层神经细胞的凋亡 ［J］. 生理学报, 2010，62（2）:109-114.
［20］Schulz JB, Weller M, Moskowitz MA. Caspases as treatment targets in stroke and neurodegenerative disease ［J］.Ann Neurol,1999,45(4):421-429.
［21］Wang ZhW, Yin YL, Zhao ChP, et al. Expression of C-fos gene and its role in vascular smooth-muscle cells after cerebral vasospasm ［J］. Chinese Journal of Neurosurgical Disease Research, 2002, l(2): 173-175. (in Chinese)
王政伟, 尹彦玲, 赵春平, 等. 脑血管痉挛后血管平滑肌细胞中C-fos基因表达及意义 ［J］. 中华神经外科疾病研究杂志, 2002, l(2): 173-175.
［22］Cunningham LA, Wetzel M, Rosenberg GA. Multiple roles for MMPs and TIMPs in cerebral ischemia ［J］. Glia, 2005, 50(4): 329-339.
［23］Rosenberg GA. Matrix metalloproteinases in neuroinflammation ［J］. Glia, 2002, 39(3): 279-291.
/
| 〈 |
|
〉 |
