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Effects of prenatal nitrite exposure on hippocampus of mouse pups
HU Sang CUI Zhan-jun GUO Jun-nan LIANG Shuang YAN Ming-chao DENG Jin-bo* DENG Jie-xin*
Acta Anatomica Sinica ›› 2015 ›› Issue (3) : 289-196.
Effects of prenatal nitrite exposure on hippocampus of mouse pups
Objective To investigate the effects on hippocampus after prenatal nitrite exposure. Methods C57BL/6J mice were used to establish the models of prenatal nitrite exposure with control group (physiological saline), low dose nitrite exposure group (60 mg/kg) and high dose nitrite exposure group (120mg/kg). The pups of different groups at P0, P7, P14 and P30 were gathered for immunofluorescence, comet assay and Western blotting analysis to detect the hippocampal injury. Results In both nitrite exposure group and control group, the number of proliferative neuron in dentate gyrus gradually decreased with the growing age. At postnatal day 0 (P0), P7, P14 and P30, after prenatal nitrite exposure, 5-bromo-2-deoxy Uridine (BrdU) positive cells decreased with dose dependency (P<0.05, n=96). In order to identify if the inhibtion was selective, we observed BrdU positive cells in subventricular zone (SVZ) at P0 mouse. After nitrite exposure, BrdU positive cells in SVZ also decreased with dose dependency (P<0.05). The inflammatory injury and apoptosis cells in hilus increased with dose dependency after nitrite exposure (P<0.05). The tail of hippocampus cells in nitrite exposure groups was longer than that in control groups with dose dependency (P<0.01). The expressions of Caspase-8 and nuclear factor κB (NF-κB) protein in nitrite exposure groups in hippocampus were much higher than that in control groups (P<0.05). Conclusion Prenatal nitrite exposure can induce various hippocampus injuries, such as neuroproliferative inhibition, oxidative stress and neuroapoptosis.
［1］Sindelar JJ, Milkowski AL. Human safety controversies surrounding nitrate and nitrite in the diet ［J］. Nitric Oxide, 2012, 26(4):259-266.
［2］Ho CK, Yang YH, Yang CY. Nitrates in drinking water and the risk of death from brain cancer: does hardness in drinking water matter ［J］ ? J Toxicol Environ Health A, 2011, 74(12):747-756.
［3］Wang JT, Zhang W, An L,et al. Nitrite poisoning inhibits the adult neurogenesis in mice［J］. Chinese Journal of Veterinary Science, 2014, 34(4):641-646.(in Chinese)
王久涛，张伟，安磊，等. 亚硝酸盐中毒抑制小鼠成体神经发生［J］. 中国兽医学报，2014，34（4）：641-646.
［4］Weng HH, Tsai SS, Wu TN, et al. Nitrates in drinking water and the risk of death from childhood brain tumors in Taiwan［J］. J Toxicol Environ Health A, 2011, 74(12):769-778.
［5］Manassaram DM, Backer LC, Messing R, et al. Nitrates in drinking water and methemoglobin levels in pregnancy: a longitudinal study［J］. Environ Health, 2010, 9:60-60.
［6］Munoz-Elias G, Woodbury D, Black IB. Marrow stromal cells, mitosis, and neuronal differentiation: stem cell and precursor functions［J］. Stem Cells, 2003, 21(4):437-448.
［7］Rojas E. Special issue on the 20th anniversary of the comet assay［J］. Mutat Res, 2009, 681(1):1-2.
［8］Cheng LC, Pastrana E, Tavazoie M, et al. miR-124 regulates adult neurogenesis in the subventricular zone stem cell niche［J］. Nat Neurosci, 2009, 12(4):399-408.
［9］Cameron HA, Mckay RDG. Adult neurogenesis produces a large pool of new granule cells in the dentate gyrus［J］. J Comp Neurol, 2001, 435(4):406-417.
［10］Puche AC, Bovetti S. Studies of adult neural stem cell migration［J］. Methods Mol Biol, 2011, 750(4):227-240.
［11］Hannah C, Kinney, Jane B, et al. Dentate gyrus abnormalities in sudden unexplained death in infants: morphological marker of underlying brain vulnerability［J］. Acta Neuropathol, 2015,129(1):65-80.
［12］Ming GL, Song HJ. Adult neurogenesis in the mammalian central nervous system［J］. Annu Rev Neurosci, 2005, 28:223-250.
［13］Wise-Faberowski L, Aono M, Pearlstein RD, et al. Apoptosis is not enhanced in primary mixed neuronal/glial cultures protected by isoflurane against N-methyl-D-aspartate excitotoxicity［J］. Anesth Analg, 2004, 99(6):1708-1714.
［14］Murugan M, Sivakumar V, Lu J, et al. Expression of N-methyl D-aspartate receptor subunits in amoeboid microglia mediates production of nitric oxide via NF-κB signaling pathway and oligodendrocyte cell death in hypoxic postnatal rats［J］. Glia, 2011, 59(4):521-539.
［15］Zhou ShF, Gao L, He WY, et al. Prenatal alcohol exposure inducing insulin resistance and stress injury in mouse hippocampi［J］. Acta Anatomica Sinica, 2014, 4(1):7-14.(in Chinese) 周淑芳，高林，贺维亚，等. 孕期酒精暴露诱导仔鼠胰岛素抵抗与海马应激损伤［J］. 解剖学报，2014，4(1)：7-14.
［16］Williams DA, Flood MH, Lewis DA, et al. Plasma levels of nitrite and nitrate in early and recent classes of fish［J］. Comp Med, 2008, 58(5):431-439.
［17］Gao X, Chen JH. Moderate traumatic brain injury promotes neural precursor proliferation without increasing neurogenesis in the adult hippocampus［J］. Exp Neurol, 2013, 239:38-48.
［18］Muscari C, Giordano E, Bonafe F, et al. Priming adult stem cells by hypoxic pretreatments for applications in regenerative medicine［J］. J Biomed Sci, 2013, 20(1):63-63.
［19］Thomas DD, Ridnour LA, Isenberg JS, et al. The chemical biology of nitric oxide: implications in cellular signaling［J］. Free Radic Biol Med, 2008, 45(1):18-31.
［20］Villalobo A. Nitric oxide and cell proliferation［J］. Febs J, 2006, 273(11):2329-2344.
［21］Jiang MR. Effects of DNA damage on cell cycle and apoptosis of mamalian cells［D］. Institute of Biochemistry and Cell Biology, SIBS, CAS, 2006.(in Chinese)
蒋满荣. DNA 损伤对哺乳动物细胞周期和凋亡的影响［D］. 中国科学院上海生命科学研究院生物化学与细胞生物学研究所， 2006.
［22］Evert BA, Salmon TB, Song BW, et al. Spontaneous DNA damage in saccharomyces cerevisiaeelicits phenotypic properties similar to cancer cells［J］. J Bio Chem, 2004, 279(21):22585-22594.
［23］Pi JB, Zhang Q, Fu JQ, et al. ROS signaling, oxidative stress and Nrf2 in pancreatic beta-cell function［J］. Toxicol Appl Pharm, 2010, 244(1):77-83.
［24］Ishisaka R, Utsumi K, Utsumi T. Involvement of lysosomal cysteine proteases in hydrogen peroxide-induced apoptosis in HL-60 cells［J］. Biosci Biotechnol Biochem, 2002, 66(9):1865-1872.
［25］Murakawa M, Jung SK, Iijima K, et al. Apoptosis-inducing protein, AIP, from parasite-infected fish induces apoptosis in mammalian cells by two different molecular mechanisms［J］. Cell Death Differ, 2001, 8(3):298-307.
［26］He YF, Zhao LP, Zhao GY, et al. The relation between osteoblast cell proliferation, apoptotic and oxidative stress under the environment of high iron［J］. Chinese Journal of Osteoporosis and Bone Mineral Sesearch, 2012, 5(2):125-129.(in Chinese)
何银锋，赵理平，赵国阳，等. 高铁环境下成骨细胞增殖和凋亡与氧化应激的关系［J］. 中华骨质疏松和骨矿盐疾病杂志，2012，5(2):125-129.
［27］Wolfe JT, Ross D, Cohon GM. A role for metals and free radicals in the induction of apoptosis in thymocytes［J］. FEBS Lett, 1994, 352(1): 58-62.
［28］Lin WC, Wei XT, Maimaiti Kelimu, et al. Studies on DNA single strand breaks induced by sodium nitroprusside-nitric oxide donor［J］. Chinese Journal of Preventive Medicine, 1999, 33(16):360-362.(in Chinese)
林慰慈，魏雪涛，买买提.克里木，等. 一氧化氮供体硝普钠致DNA单链断裂的试验研究［J］. 中华预防医学杂志，1999，33(16)：360-362.
［29］Zhang BG, Peng XL, Li GH, et al. Oxidative stress is involved in Patulin induced apoptosis in HEK 293 cells［J］. Toxicon, 2015, 94:1-7.
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