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Effect of G protein-coupled receptor 30 on the distribution and expression of toll-like receptor 4 in the uterus of ovariectomized mice
LIU Hong-gai WANG Lu DAI Ying-ying YU De-shui ZHAO Hui-ying*
Acta Anatomica Sinica ›› 2015, Vol. 46 ›› Issue (2) : 265-269.
Effect of G protein-coupled receptor 30 on the distribution and expression of toll-like receptor 4 in the uterus of ovariectomized mice
Objective To investigate the role of G protein-coupled receptor 30（GPR30）on the uterine immunity. Methods A total of 80 mice were used in this study. Immunochemistry SP method and fluorescent real-time quantitative PCR technology were used to study the distribution of toll-like receptor（TLR4）and the expression of TLR mRNA in sham group, ovariectomized (OVX) group, OVX+0.1nmol G1 group, OVX+0.5nmol G1 group, OVX+2.5nmol G1 group, OVX+0.1nmol G15 group, OVX+0.5nmol G15 group, OVX+2.5nmol G15 group. Results TLR4 was mostly localized to the cell membrane and cytoplasm of the luminal, glandular epitheliumcells and mesenchymal cells. The intensities of the immunohistochemical positive staining for TLR4 were significantly lower in G1 high concentration group than those in other groups(P<0.01). After administrating the antogonist G15 to the OVX mice, the relative expression of TLR4 was increased with G15 concentration increasing. The significant difference on the relative expression of TLR4 in OVX+G15 group was coincidence with OVX+G1 group. The result of fluorescent realtime quantitative PCR showed that the expression level change of TLR4 mRNA in uterus of mice between groups was consistent with the relative expression of TLR4. Conclusion These results indicate that GPR30 can inhibit the expression of TLR4 in the uterus，and that GPR30 may participate in regulating the uterus local immune function.
G protein-coupled receptor 30 / Uterus / Toll-like receptor 4 / Immunochemical SP method / Real-time PCR / Ovariectomized mouse
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