Welcome to visit Acta Anatomica Sinica! Today is
Chinese
Effects of angelica sinensis polysaccharide on the spleen structure and function of aging rats
ZHANG Meng-si ZOU Ting YE Yuan-wen JIA Dao-yong ZHANG Yan-yan WANG Ya-ping*
Acta Anatomica Sinica ›› 2015, Vol. 46 ›› Issue (2) : 257-264.
Effects of angelica sinensis polysaccharide on the spleen structure and function of aging rats
Objective To explore the effect of angelica sinensis polysaccharide(ASP) on the spleen structure and function of aging rats，and provide theoretical and experimental evidences for seeking the effective ingredient from natural medicine to delay immune system aging. Methods Forty SD rats were randomly divided into normal control group, ASP control group, aging model group and ASP aging model group. Aging model group were treated with D-galactose ［120 mg/(kg·d)］ for 42 days by subcutaneous injection. ASP aging group were also injected with D-galactose with the same dose and time as aging model group, and from the 14th day on, rats were given ASP (100 mg/kg) by intraperitoneal injection for 28 days. Normal control group were received saline with the same volume for 42 days. ASP control group were given saline with the same volume for 14 days, and received ASP (100mg/kg) by intraperitoneal injection for 28 days. After 2 days of finishing the treatment,the spleen index was measured, paraffin section was made to observe spleen microscopic structures；The ratio of the SA-β-Gal staining positive splenocytes were counted；the proliferative capacity of splenocytes with stimulating by concanavalin A (Con A) was detected by CCK8；the distribution of cell cycles and ROS levels was analyzed by flow cytometry(FCM)；The capability of splenocyte to secrete TNF-α, GM-CSF, malondialdehyde(MDA), superoxide dismutase (SOD)were assayed with ELISA；The aging related protein P53, P21, RB were detected by Western blotting. Results Comparing the aging rats induced by D-galactose with normal control group, the following biological features of spleens in aging model group: spleen index, splenic white pulp area proportion, the proliferative capacity of splenocytes stimulated by Con A, the ratio of S stages in cell cycle , the secretory capability of TNF-α and GM-CSF, and the active content of SOD, were obviously decreased. The percentage of SA-β-Gal positive cells, the ratio of G1 and G2/M stages, the product of ROS and MDA in splenocytes were significantly increased. The expressions of P53, P21, RB were up-regulated. Comparing ASP aging group with aging model group, ASP remarkably increased spleen index, splenic white pulp area proportion, the proliferation of splenocytes stimulated by Con A, the ratio of S stages, the secretory capability of TNF-α and GM-CSF, the active content of SOD. ASP obviously increased the percentage of SA-β-Gal positive cells, the ratio of G1 and G2/M stages, the product of ROS and MDA in splenocytes, and down-regulate the expression of P53, P21, RB. Conclusion The spleen structure and function are obviously damaged in D-galactose-induced aging model rats.ASP has definitely protective effects on the injury.
Angelica polysaccharide / D-galactose / Aging model / Spleen / Western blotting / Rat
［1］Wagner WM,Ouyang Q,Sekeri-Pataryas K,et al.Basic biology and clinical impact of immunosenescence［J］.Biogerontology,2004,5(1):63-66.
［2］Cao W,Li XQ,Wang X,et al.A novel polysaccharide,isolated from Angelical sinensis (Oliv.) Diels induces the apoptosis of cervical cancer HeLa cells through an intrinsic apoptosis pathway［J］. Phytomedicine,2010,17(89):598-605.
［3］Liu C,Li J,Meng FY,et al. Polysaccharides from the root of aAngelica sinensis promotes hematopoiesis and thrombopoiesis through the PI3K/AKT pathway［J］.BMC Complement Altem Med,2010,10:79.
［4］He XL,Guan XJ,Wu H,et al.Effects of angelica polysaccharide on oxidative damage and apoptosis of bone marrow mononuclear cell in radiation injured mice［J］.Journal of Medical University of Chongqing,2012,37(4) :315-318. (in Chinese)
何晓莉,关雪晶,吴宏,等.当归多糖对电离辐射致小鼠骨髓单个核细胞凋亡及氧化损伤的影响［J］.重庆医科大学学报,2012,37(4) :315-318.
［5］Liu J, Xu CY, Cai SZ, et al. Senescence effect of angelica sinensis polysaccharide on human acute myelogenous leukemia stem and progenitor cell［J］.Asian Pac J Cancer Preve,2013,14(11):6549-6556.
［6］Peng B,Chen MSh,Pu Y,et al.Anti-aging effects of Ginsenoside Rg1 and it’s mechanisms on brain aging rats induced by D-galactose［J］Journal of Medical University of Chongqing,2011,36(4):419-422. (in Chinese)
彭彬,陈茂山,蒲莹,等.人参皂苷Rgl延缓D-半乳糖大鼠脑衰老作用及机制的初步研究［J］.重庆医科大学学报,2011,36(4):419-422.
［7］Gorczynski RM, Terzioglu E. Aging and the immune system［J］. Int Urol Nephrol, 2008, 40(4): 1117-1125.
［8］Weng NP.Aging of the immune system : how much can the adaptive immune system adapt［J］?Immunity, 2006, 24(5):495-499.
［9］Dai DF,Rabinovitch PS,Ungvari Z.Mitochondria and cardiovascular aging［J］.Circ Res,2012,110(8):1109-1124.
［10］Droge W.Free radicals in the physiological control of cell function［J］.Physiol Rev,2002,82(1):47-95
［11］Takeuchi A,Mishina Y,Miyaishi O,et al.Heterozygosity with respect to Zfp 148 causes complete loss of fetal germ cells during mouse embryogenesis［J］.Nat Genet,2003,33(2):172-176.
［12］Simon HU,Haj-Yehia A,Levi-Schaffer F.Role of reactive oxygen species(ROS)in apoptosis induction［J］.Apoptosis,2000,5(5):415-418.
［13］Szibor M,Richter C,Ghafourifar P.Redox control of mitochondrial functions［J］.Antioxid Redox Signal,2001,3(3):515-523.
［14］Xu XL, Zhang ZG,Shen XY,et al.The influence of fluoresis on learning-memory behaviors of mice and the activities of SOD and the content of MDA in the brain［J］.China Public Health,2001,17(1):8-9. (in Chinese)
许晓路,章子贵，申秀英，等.氟中毒对小鼠学习记忆及脑内SOD活性和MDA含量的影响［J］.中国公共卫生,2001，17(1):8-9.
［15］Lin H,Wang K.Metabolism of oxide free-radical and NO,SOD,MDA alteration of serum lipid and hemorrheology in elderly niddm［J］. Gereatrics and Health Care,2000,6(2):96-98.(in Chinese)
林桦,王凯.老年2型糖尿病患者氧自由基代谢、血清NO、SOD、MDA及血脂、血流变指标关系探讨［J］.老年医学与保健,2000,6(2):96-98.
［16］Wu KF,Hu Y.Establishment and evaluation of D-galactose induced aging animal model［J］. Chinese Journal of Multiple Organ Diseases in the Elderly, 2012,11(1):74-80.(in Chinese)
吴克芬,胡予.D-半乳糖致衰老动物模型的建立及评价［J］.中华老年多器官疾病杂志,2012,11(1):74-80.
［17］Tarazona R,Solana R,Ouyang Q, et al.Basic biology and clinical impact of immunosenescence［J］.Exp Gerontol,2002,37(2-3):183-189.
［18］Oda E,Ohki R,Murasawa H,et al.Noxa,a BH3-only member of the Bcl-2 family and candidate mediator of p53-induced apoptosis［J］.Science,2000,288(5468):1053-1058.
［19］Chen X,Zhang W,Gao Y,et al.Senescence-like changes induced by expression of p21(waf1/Cip1) in NIH3T3 cell line［J］.Cell Res,2002,12(34):229-233.
［20］Jackson JG,Pereira-Smith OM.p53 is preferentially recruited to the promoters of growth arrest genes p21 and GADD45 during replicative senescence of normal human fibroblasts［J］.Cancer Res,2006,66(17):8356-8360.
National Natural Science Fund
/
| 〈 |
|
〉 |
