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Expression patterns of lamin A, TBX3 and connexin 43 in developing mouse embryonic hearts
SHI Rui JING Ya* SHI Liang YANG Yan-ping LIU Hui-xia SONG Li
Acta Anatomica Sinica ›› 2015, Vol. 46 ›› Issue (2) : 238-243.
Expression patterns of lamin A, TBX3 and connexin 43 in developing mouse embryonic hearts
Objective To explore the morphogenesis and differentiation of working myocardium and conduction myocardium in mouse embryonic heart and expression patterns of lamin A, TBX3 and connexin 43 (Cx43). Methods Both the immunohistochemical and immunofluorescent methods were used to observe the relationship of α-smooth muscle actin(α-SMA), myosin heavy chain(MHC), islet-1(ISL-1), Cx43, lamin A and TBX3 distribution patterns in 46 mice embryos from embryonic day(ED)8 to 15 with the myocardial differentiation. Results At ED9, strong TBX3 expression was mainly displayed in the myocardium of the atrioventricular canal. From ED10 onwards, TBX3 expression extended towards the sinus node along the venous valves and the dorsal wall of the right atrium, including interatrial septum. At ED12-13, the prototype of central conduction system of embryonic heart composed by the sinus node, the left and right venous valves, interatrial septum, atrioventricular canal, atrioventricular node and the atrioventricular bundle was recognized, which showed TBX3 positive expression. Cx43 weak expression first appeared in the ventral wall of the left ventricle and part of the trabecular myocardium at ED9. With the development, the expression of Cx43 was displayed in the atrial and ventricular working myocardium with the TBX3 negative expression. Lamin A expression first appeared in ectomesenchymal cells of atrioventricular canal endocardial cushion and part of the left ventricular trabecular myocardium at ED10. Then the expression of lamin A displayed in the right ventricular trabecular myocardium. At ED15, the positive expression of lamin A distributed in the atrioventricular valves, ventricular and atrial trabecular myocardium. However, lamin A expression in compact myocardium and central conduction system remained negative. Conclusion The prototype of central conduction system is formed at ED13, showing TBX3 positive expression and Cx43 negative expression, which is a kind of complementary expression. The expression of lamin A in the compact myocardium and central conduction system myocardium remain negative at ED15, indicating that these parts of myocardium are matured later.
Lamin A / TBX3 / Connexin 43 / Working myocardium / Conduction myocardium / Immunohistochemisty / Immunofluorescence / Mouse
［1］Hoogaars WM, Tessari A, Moorman AF, et al. The transcriptional repressor Tbx3 delineates the developing central conduction system of the heart ［J］. Cardiovasc Res, 2004, 62(3):489-499.
［2］Bakker ML, Boukens BJ, Mommersteeg MT, et al. Transcription factor Tbx3 is required for the specification of the atrioventricular conduction system ［J］.Circ Res, 2008, 102(11):1340-1349.
［3］Gourdie RG, Severs NJ, Green CR, et al. The spatial distribution and relative abundance of gap-junctional connexin 40 and connexin43 correlate to functional properties of components of the cardiac atrioventricular conduction system ［J］. J Cell Sci, 1993, 105(4):985-991.
［4］Jung HJ, Coffinier C, Choe Y, et al. Regulation of prelamin A but not lamin C by miR-9, a brain-specific microRNA ［J］, Proc Natl Acad Sci USA, 2012, 109(7):423-431.
［5］Park DS, Fishman GI. The Cardiac Conduction System ［J］. Circulation, 2011, 123(8):904-915.
［6］Bertrand AT, Renou L, Papadopoulos A, et al. DelK32-lamin A/C has abnormal location and induces incomplete tissue maturation and severe metabolic defects leading to premature death ［J］. Hum Mol Genet, 2012, 21(5):1037-1048.
［7］Mounkes LC, Kozlov SV, Rottman JN, et al. Expression of an LMNA-N195K variant of A-type lamins results in cardiac conduction defects and death in mice ［J］. Hum Mol Genet, 2005, 14(15):2167-2180.
［8］Lakdawala NK, Winterfield JR, Funke BH. Dilated Cardiomyopathy［J］. Circ Arrhythm Electrophysiol, 2013, 6(1):228-237.
［9］Qiao AX, Jing Y, Cai YJ, et al. Expression patterns of hyperpolarization-activated cyclic nucleotide-gated cation 4, connexin 43 and podoplanin in the embryonic mouse heart and development of cardiac conduction system ［J］. Acta Anatomica Sinica, 2012, 43(3):405-411. (in Chinese)
乔爱秀,景雅,蔡玉瑾,等.超极化激活环核苷酸门控阳离子通道4、连接蛋白43和平足蛋白在小鼠胚胎心的表达与心传导系的发生［J］.解剖学报,2012,43(3):405-411.
［10］Li HCh, Jing Y, Shi L, et al. Association of morphogenesis of pulmonary endoderm with development of prepharyngeal mesenchyme and outflow tract septation in mouse embryos ［J］. Acta Anatomica Sinica, 2013, 44(6):804-811. (in Chinese)
李慧超,景雅,师亮,等.小鼠胚胎呼吸内胚层形态发生与咽前间充质发育及流出道分隔的关系［J］.解剖学报,2013,44(6):804-811.
［11］Munshi NV. Gene regulatory networks in cardiac conduction system development ［J］. Circ Res, 2012, 110(11):1525-1537.
［12］Blom NA, Gittenberger-de Groot AC, DeRuiter MC, et al. Development of the cardiac conduction tissue in human embryos using HNK-1 antigen expression: possible relevance for understanding of abnormal atrial automaticity ［J］.Circulation, 1999, 99(6):800-806.
［13］Rentschler S, Vaidya DM, Tamaddon H, et al. Visualization and functional characterization of the developing murine cardiac conduction system ［J］. Development, 2001, 128(10):1785-1792.
［14］Erokhina IL, Rumyantsev PP. Proliferation and biosynthetic activities of myocytes from conductive system and working myocardium of the developing mouse heart. Light microscopic autoradiographic study ［J］. Acta Histochem, 1988, 84(1):51-66.
［15］Morris GM, D’Souza A, Dobrzynski H, et al. Characterization of a right atrial subsidiary pacemaker and acceleration of the pacing rate by HCN over-expression ［J］. Cardiovasc Res, 2013, 100(1):160-169.
［16］Nikolova V, Leimena C, McMahon AC, et al. Defects in nuclear structure and function promote dilated cardiomyopathy in lamin A/C-deficient mice ［J］. J Clin Invest, 2004, 113(3):357-369.
［17］Mavroudis C, Backer CL. Pediatric Cardiac Surgery ［M］.4th ed. US: Wiley, 2013: 1-26.
［18］Holaska JM. Emerin and the nuclear lamina in muscle and cardiac disease ［J］. Circ Res, 2008, 103(1):16-23.
［19］Dahl KN, Kalinowski A. Nucleoskeleton mechanics at a glance ［J］. J Cell Sci, 2011, 124(5):675-678.
［20］Li HR, Jing Y, Xu XW, et al. Association of α-SCA, α-SMA and desmin with the myocardial maturation of the embryonic mouse heart ［J］. Acta Anatomica Sinica, 2005, 36(4): 422-427. (in Chinese)
李海荣，景雅，徐秀文，等. α-SCA、α-SMA和结蛋白与小鼠胚胎心肌发育成熟的关系［J］. 解剖学报，2005，36（4）：422-427.
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