Welcome to visit Acta Anatomica Sinica! Today is
Chinese
Distribution of protein gene product 9.5 and neuropeptide Y in yak testis at different ages
YUAN Li-gang* QU Ya-ling GU Lai-feng TIAN Dan-zeng
Acta Anatomica Sinica ›› 2015, Vol. 46 ›› Issue (2) : 232-237.
Distribution of protein gene product 9.5 and neuropeptide Y in yak testis at different ages
Objective To investigate the distribution of gene product 9.5 and neuropeptide Y(NP-Y) in yak testis and in an attempt of exploring their relationships with testicular spermatogenesis at different ages. Methods Using immunohistochemical SP technique, we depicted the distribution characteristics of PGP 9.5 and NP-Y in yak testis: 9 pairs of yak calves in age from 3 to 4 months, 10 pairs of young adult yak from 3 to 4 years and 7 pairs of aging yak from 9 to 13 years old were prepared by orchiectomy surgery, bedded in paraffin and sectioned serially. Results NP-Y and PGP 9.5 immunoreactivities were abundantly distributed in gonads, mainly associated with Leydig cell nests, blood vessels, and seminiferous tubules. PGP 9.5 and NP-Y were mainly expressed in spermatogonia of yak calves testis and in spermatogenic cells of young yak testis. By contrast, no NP-Y and PGP 9.5 immunoreactivities were observed within the primary spermatocytes. The expression of PGP 9.5 and NP-Y in corpus testis were stronger than the caput and cauda of the testis. A small number of PGP 9.5-immunoreactive cells was detected in testis vscellum of aging yak. Conclusion Our results suggest that the PGP 9.5 and NP-Y expression is significantly different in young adult yak testis and may serve as regulators for testicular functions.The significant dcrease of distribution of NP-Y in the wall of vascular structure in aging yak testis.
Protein gene product 9.5 / / Neuropeptide Y / / Testis / / Age / / Immunohistochemistry / Yak
［1］Tainio H.Peptidergic innervation of the human testis and epididymis ［J］. Acta Histochem，1994,96(4):415-420.
［2］Gong YG, Feng MM, Hu XN, et al. Peptidergic not monoaminergic fibers profusely innervate the young adult human testis［J］.J Anat， 2009, 214(3): 330-338.
［3］Wrobel KH, Brandl B. The autonomous innervation of the porcine testis in the period from birth to adulthood［J］.Ann Anat,1998,180(2): 145-156.
［4］Saleh AMM, Alameldin MA, Abdelmoniem ME, et al. Immunohistochemical investigations of the autonomous nerve distribution in the testis of the camel(Camelus dromedarius)［J］. Ann Anat, 2002, 184(3): 209-220.
［5］Wrobel KH, Gurtler A. The nerve distribution in the testis of the cat ［J］. Ann Anat, 2001, 183(4): 297-308.
［6］Zeng XZh, Shen HJ, Wang CR, et al. Changes of neuropeptide Y and synapses in hippocampus after hypoxia preconditioning in mice ［J］. Acta Anatomica Sinica, 2011, 42(2): 155-158.(in Chinese)
曾宪智,沈慧娟,王从荣, 等.低氧预适应后小鼠海马神经肽Y及突触的变化［J］.解剖学报, 2011, 42(2): 155-158.
［7］Wrobel KH, Abu-Ghali N. Autonomic innervation of the bovine testis.［J］. Acta Anat, 1997, 160(1): 1-14.
［8］Gruenewald DA, Naai MA, Marck BT, et al. Agerelated decrease in neuropeptide-Y gene expression in the arcuate nucleus of the male rat brain is independent of testicular feedback［J］.Endocrinology, 1994,134(6):2383-2389.
［9］Yan P, Pan HP. Environmental effects on yak reproductive performance［J］. Animal Husbandry and Veterinary Medicine, 2004, 36(5):15-16.(in Chinese)
阎萍,潘和平.环境因素对牦牛繁殖性能的影响［J］. 畜牧与兽医,2004,36(5):15-16.
［10］Chow SH, Giglio W, Anesetti R, et al. The effects of testicular denervation on spermatogenesis in the Sprague-Dawley rat［J］. Neuroendocrinology, 2000, 72(1): 37-45.
［11］Heidari B, Rahmati-Ahmadabadi M, Akhondi MM, et al. Isolation, identification, and culture of goat spermatogonial stem cells using c-kit and PGP 9.5 markers［J］.J Assist Reprod Genet ,2012, 29(10):1029-1038
［12］Wei L, Luo LQ, Peng KM, et al. Morphologic characteristics of testis and distribution of NP-Y in testis of the ostrich chicks.［J］. Chinese Journal of Veterinary Science, 2009,29(12): 1599-1602. (in Chinese)
位兰,罗来强,彭克美.雏鸵鸟睾丸的结构及睾丸内神经肽Y的表达.［J］.中国兽医学报, 2009, 29(12): 1599-1602.
［13］Terado M, Nomura M, Mineta K, et al. Expression of Neuropeptide Y gene in mouse testes during testicular development.［J］.Asian J Androl, 2006,8(4):443-449.
［14］Heidari B, Gifani M, Shirazi A, et al. Enrichment of undifferentiated type a spermatogonia from goat testis using discontinuous percoll density gradient and differential plating［J］. Avicenna J Med Biotechnol, 2014 ,6(2):94-103.
［15］Kanzaki M1, Fujisawa M, Okuda Y, et al. Expression and regulation of neuropeptide Y messenger ribonucleic acid in cultured immature rat Leydig and Sertoli cells［J］.Endocrinology, 1996 ,137(4):1249-1257.
［16］Kon Y, Endoh D, Iwanaga T. Expression of protein gene product 9.5, a neuronal ubiquitin C-terminal hydrolase, and its developing change in sertoli cells of mouse testis［J］. Mol Reprod Dev, 1999,54(4):333-341.
［17］Davidoff MS, Schulze W, Middendorff R, et al. The Leydig cell of the human testis-a new member of the diffuse neuroendocrine system［J］.Cell Tissue Research,1993, 271(3): 429-439.
［18］Liao W, Cai M, Chen J,et al. Hypobaric hypoxia causes deleterious effects on spermatogenesis in rats［J］.Reproduction, 2010,139(6): 1031-1038.
［19］Verratti V, Di Giulio C, Berardinelli F, et al. Pampiniform plexus and oxidative stress during chronic hypoxia and hyperoxia ［J］. Int J Immunopathol Pharmacol, 2008, 21(2):353-357.
/
| 〈 |
|
〉 |
