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Expression and effect of let-7a, miR-21 and miR-27a in oral squamous cell carcinomas
LIU Nai-guo* ZHANG Wei-qun HU Feng-ai WU Shu-hua- ZHU Yu-hong
Acta Anatomica Sinica ›› 2015, Vol. 46 ›› Issue (2) : 221-226.
Expression and effect of let-7a, miR-21 and miR-27a in oral squamous cell carcinomas
Objective To explore the expression and function of let-7a, miR-21 and miR-27a in oral squamous cell carcinomas(OSCCs). Methods Using in situhybridization by digmarked miRCURY LNATM microRNA detection probes, the expressions levels of let-7a, miR-21 and miR-27a were studied on nature and localization in 5 normal oral mucous tissues, 30 OSCCs and their paired pericarcinomatous tissues. The results of microRNAs expression were semi-quantitatively analyzed by image analysis. Results The expressions of let-7a, miR-21 and miR-27a were weak, and only found in 40%(2/5), 80% (4/5) and 40%(2/5) normal oral mucous tissues, respectively. But all the tumor tissues and their pericarcinomatous tissues expressed the three miRNAs in different extent. The expression levels of three miRNAs in OSCCs were much higher than that in pericarcinomatous tissues and normal tissues (P<0.01). The expression of let-7a and miR-27a in pericarcinomatous tissues was obviously higher than that in normal tissues (P<0.01), however, no obvious difference was observed between the expression of miR-21 in pericarcinomatous tissues and in normal tissues (P>0.05). No significant difference was detected in OSCC patients with different age and sex, and lymphatic metastasis or not (P>0.05). The expression of miR-27a in moderate/low differentiated OSCCs was higher than its expression in high differentiated OSCCs（P<0.05）. No statistical difference of miR-21 and let-7a expression was found in differentiation-different OSCCs (P>0.05). Correlation analysis showed that there was positive correlation between expression of let-7a and miR-21 in OSCCs, but no correlation was found between expression of miR-27a and let7a/miR-21.
Conclusion The overexpression patterns of let-7a, miR-21 and miR-27a play a certain role in tumorgenesis and development of OSCCs. miR-27a is an expected molecular marker associated with differentiated degree of OSCCs. There is a certain relation between let-7a and miR-21 in OSCCs.
Oral squamous cell carcinomas / let-7a / miR-21 / miR-27a / In situ hybridization / Image analysis / Human
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