Welcome to visit Acta Anatomica Sinica! Today is
Chinese
Reelin and the cerebellar development—— the regulatory effect of Notch1 signaling pathways
YAN Ming-chao NIU Yan-li WANG Xiao-qing LIANG Shuang GUO Jun-nan SHI Shu-qin HU Sang DENG Jin-bo*
Acta Anatomica Sinica ›› 2015, Vol. 46 ›› Issue (2) : 182-189.
Reelin and the cerebellar development—— the regulatory effect of Notch1 signaling pathways
Objective To investigate the effect of Reelin on the neural migration, distribution of radial glial cells, cell migration and Purkinje cells’ development in the cerebellum, and to discuss. Regulatory effects of Notch signaling pathways. Methods A total of 148 wild-type (WT) and reeler mice from E9 (embryonic day 9) to P60 (postnatal day 60) were used for immunofluoresent labeling to visualize, the radial glial cells, neural stem cells, Purkinje cells and Notch1 positive cell in the developing cerebellum. Results Comparing with WT mice, in reeler mice, the orientations of radial glial cells (putative Bergmann cell) were disordered with number decrease (P<0.01). The cell bodies of Bergmann cells did not move into their proper position and lingered around in internal granular layer(IGL). Sox2-positive stem cells in external granular layer migrated into internal granular layer with time-lag. Purkinje cells did not reach their destination and accumulated in internal granular layer. We found that Notch1 positive cells reduced in reeler mice. Conclusion Reelin plays an important role in the development of radial glial cells (Bergmann cells), cell migration and neural proliferation. In addition, Notch1 signaling pathway is involved in cerebellar development, via the regulation to Reelin pathway.
Reelin / Notch1 receptor / Cerebellar development / Neural stem cells / Immunofluorescence / Mouse
［1］Rice DS, Curran T. Role of the reelin signaling pathway in central nervous system development［J］. Annu Rev Neurosci, 2001, 24(1): 1005-1039.
［2］Zhao S, Frotscher M. Go or stop? Divergent roles of Reelin in radial neuronal migration［J］. Neuroscientist, 2010, 16(4):421-434.
［3］Ogawa M, Miyata T, Nakajima K, et al. The reeler gene-associated antigen on Cajal-Retzius neurons is a crucial molecule for laminar organization of cortical neurons［J］. Neuron, 1995, 14(5):899-912.
［4］Deng JB, Xu X B, Fan W J. Neocortical development and formation of lamination［J］. Progress of Anatomical Sciences, 2008, (04):423-428,431. (in Chinese)
邓锦波, 徐晓波, 范文娟. 新皮质的发育与片层化构筑的形成［J］. 解剖科学进展, 2008, (04):423-428,431.
［5］Lee H, Song MR. The structural role of radial glial endfeet in confining spinal motor neuron somata is controlled by the Reelin and Notch pathways［J］. Exp Neurol, 2013, 249:83-94.
［6］Bray SJ. Notch signalling: a simple pathway becomes complex［J］. Nat Rev Mol Cell Biol, 2006, 7(9):678-689.
［7］Fu X, Shi ZhY, Jin HX, et al. The effects of hippocampal microenvironment on cells’ differentiation and polarity［J］. Acta Anatomica Sinica, 2013, 44(5):586-593. (in Chinese)
符星, 石贞玉, 金海啸, 等. 海马微环境对细胞分化和极性化的影响［J］. 解剖学报, 2013, 44(5):586-593.
［8］Hawthorne AL. Repurposing Reelin: the new role of radial glia, Reelin and Notch in motor neuron migration［J］. Exp Neurol, 2014, 256:17-20.
［9］D’Arcangelo G, Miao GG, Chen SC, et al. A protein related to extracellular matrix proteins deleted in the mouse mutant reeler［J］. Nature, 1995, 374(6524):719-723.
［10］Hirotsune S, Takahara T, Sasaki N, et al. The reeler gene encodes a protein with an EGF-like motif expressed by pioneer neurons［J］. Nat Genet, 1995, 10(1):77-83.
［11］D’Arcangelo G, Homayouni R, Keshvara L, et al. Reelin is a ligand for lipoprotein receptors［J］. Neuron, 1999, 24(2):471-479.
［12］Howell BW, Herrick TM, Hildebrand JD, et al. Dab1 tyrosine phosphorylation sites relay positional signals during mouse brain development［J］. Curr Biol, 2000, 10(15):877-885.
［13］Lakoma J, Garcia-Alonso L, Luque JM. Reelin sets the pace of neocortical neurogenesis［J］. Development, 2011, 138(23):5223-5234.
［14］Dong Z, Yang N, Yeo SY, et al. Intralineage directional Notch signaling regulates self-renewal and differentiation of asymmetrically dividing radial glia［J］. Neuron, 2012, 74(1):65-78.
［15］Keilani S, Healey D, Sugaya K. Reelin regulates differentiation of neural stem cells by activation of notch signaling through Disabled-1 tyrosine phosphorylation［J］. Can J Physiol Pharmacol, 2012, 90(3):361-369.
［16］Kipp M, Gingele S, Pott F, et al. BLBP-expression in astrocytes during experimental demyelination and in human multiple sclerosis lesions［J］. Brain Behav Immun, 2011, 25(8):1554-1568.
［17］Kim H, Shin J, Kim S, et al. Notch-regulated oligodendrocyte specification from radial glia in the spinal cord of zebrafish embryos［J］. Dev Dyn, 2008, 237(8):2081-2089.
［18］Qin JB, Cheng M, Jin GH, et al. Effects of Jagged1 on hippocampal radial glial cells’ proliferation and neuronal differentiation［J］. Acta Anatomica Sinica, 2014, (5):585-590. (in Chinese)
秦建兵, 成敏, 金国华, 等. Jagged1对海马放射状胶质细胞增殖和向神经元分化的影响［J］. 解剖学报, 2014, (5):585-590.
［19］Keilani S, Sugaya K. Reelin induces a radial glial phenotype in human neural progenitor cells by activation of Notch-1［J］. BMC Dev Biol, 2008, 8:69.
［20］Deng JB, Xu XB, Fan WJ. Development and gene regulation of cerebellum［J］. Progress of Anatomical Sciences, 2010,16(2):181-186. (in Chinese)
邓锦波, 徐晓波, 范文娟. 小脑发育及其基因调节［J］. 解剖科学进展, 2010, 16(2):181-186.
［21］Tissir F, Goffinet AM. Reelin and brain development［J］. Nat Rev Neurosci, 2003, 4(6):496-505.
［22］Hack I, Hellwig S, Junghans D, et al. Divergent roles of ApoER2 and Vldlr in the migration of cortical neurons［J］. Development, 2007, 134(21):3883-3891.
［23］Benhayon D, Magdaleno S, Curran T. Binding of purified Reelin to ApoER2 and VLDLR mediates tyrosine phosphorylation of Disabled-1［J］. Brain Res Mol Brain Res, 2003, 112(1-2):33-45.
［24］Niu BH, Zhang Y, Niu YL, et al.Radial glial cell and development of cerebellar cortex in mouse［J］. Acta Anatomica Sinica, 2010, 41(4):498-503. (in Chinese)
牛保华, 张艳, 牛艳丽, 等. 小鼠放射状胶质细胞和小脑皮质的发育［J］. 解剖学报, 2010, 41(4):498-503.
［25］Cheng XSh, Jiang QY, Hu YQ, et al. Histological origin in mouse cerebellar cortex［J］. Chinese Journal of Anatomy, 2007, 30(05): 576-581. (in Chinese)
程相树, 蒋杞英, 胡艳秋, 等. 小鼠小脑皮质的组织发生［J］. 解剖学杂志, 2007,30(05):576-581.
［26］Artavanis-Tsakonas S, Rand MD, Lake RJ. Notch signaling: cell fate control and signal integration in development［J］. Science, 1999,284(5415):770-776.
［27］Artavanis-Tsakonas S, Matsuno K, Fortini ME. Notch signaling［J］. Science, 1995, 268(5208):225-232.
［28］Hashimoto-Torii K, Torii M, Sarkisian MR, et al. Interaction between Reelin and Notch signaling regulates neuronal migration in the cerebral cortex［J］. Neuron, 2008, 60(2):273-284.
/
| 〈 |
|
〉 |
