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Effect and mechanism of Heshowuyin on alleviating the hippocampal neurons injury induced by β-amyloid protein
CHEN Jing-bo WANG Yu-juan GUO Sheng-nan NIU Si-yun* DUAN Fei*
Acta Anatomica Sinica ›› 2015, Vol. 46 ›› Issue (2) : 175-181.
Effect and mechanism of Heshowuyin on alleviating the hippocampal neurons injury induced by β-amyloid protein
Objective To investigate the protective effect of Heshouwuyin on primary hippocampal neuronal cells induced by β-amyloid protein（Aβ）and its possible mechanisms. Methods Hippocampal neurons collected were from newborn SD rats aged in 24 hours.Hippocampal cells which were cultured 10 days were divided into five groups:Model group,Heshouwuyin prevention group,Heshouwuyin treatment group,Heshouwuyin control group and Normal control group.Hoechst33258 staining was used to observe the apoptosis rate of the hippocampal cells.Immunohistochemistry staining was used to observe the expression of Trx1.The mRNA expression of Trx1 was detected in the hippocampal neuronal cells of each group with reverse transcription polymerase chain reaction , and the protein expression of Trx1 was detected in the hippocampal neuronal cells with Western blotting. Results The apoptosis rate of hippocampal neuronal cells was significantly increased in the Model group (P<0.05).In the Model group,the expresstion of Trx1 was significantly decreased(P<0.05).Compared with the Model group,the apoptosis rate of hippocampal neuronal cells was significantly decreased in the Heshouwuyin prevention group and Heshouwuyin treatment group(P<0.05).The mRNA and protein expresstions of Trx1 were significantly higher in Heshouwuyin prevention group and Heshouwuyin treatment group than that in the Model group(P<0.05).Conclusion Heshouwuyin can significantly alleviate the Hippocampal neurons injury induced by Aβ. The neuroprotective activities of Heshouwuyin can be related to its ability to increase the Trx1 expression level.
Hippocampal neuron / Alzheimer’s disease / β-amyloid protein / / Heshouwuyin / / Thioredoxin / Immunohistochemistry / Rat
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