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The influence of superior cervical ganglionectomy on expression of matrix metalloproteinase-9 and aquaporins-9 in the hippocampus in adult rats
ZHAO Qian-nan DING Yan-ping* SHAO Bao-ping ZHANG Jin-ping LIU Jian-feng JIA Ling-yun
Acta Anatomica Sinica ›› 2015, Vol. 46 ›› Issue (2) : 158-163.
The influence of superior cervical ganglionectomy on expression of matrix metalloproteinase-9 and aquaporins-9 in the hippocampus in adult rats
Objective To study the expression of matrix metalloproteinase-9 (MMP9), aquaporins-9 (AQP9）in the hippocampus after the superior cervical ganglionectomy in adult rats, and to explore the regulate mechanism of sympathetic ganglion on the brain tissue. Methods A total of 40 healthy adult male Wistar rats were divided into control and operation groups. The surgery of superior cervical ganglionectomy was perfomed in the operation group. Immunohistochemistry (IHC) was used to localize the expression of MMP9 and AQP9 in the hippocampus. Western-blotting was used to quantify the expression change of MMP9 and AQP9. RT-PCR was used to detect the variation of MMP9 mRNA and AQP9 mRNA in the hippocampus. Results MMP9 immunohistochemical product was mainly expressed in neurons and glial cells. The expression of MMP9 in hippocampus of the operation group was significantly stronger than the control group. The few positive neurons in CA1 and CA3 were scattered in the pyramidal cell layer , and strongly positive cells were mainly located in the granular layer of dentat gyrus(DG) region. Compared to the control group, the MMP9 protein expression and MMP9 mRNA expression levels in the hippocampus of the operate group were significantly increased (P<0.01). AQP9 immunohistochemical product was mainly expressed in neurons, glial cells, and blood vessels; the expression of AQP9 in the hippocampus of the operation group was significantly stronger than the control group. The positive cells were mainly located in new born cells of subgranular zone of DG region and ependymal cells. Compared to the control group, the AQP9 protein expression and AQP9 mRNA expression levels in the hippocampus of the operate group were significantly increased (P<0.01) than control group. Conclusion The expressions of MMP9 and AQP9 are significantly increased in the hippocampus after 15 days of the superior cervical ganglionectomy surgery, indicating that removal of sympathetic ganglions may change cerebral blood supply and further affect the expression of some related factors in the hippocampus.
Superior cervical ganglion / Hippocampus / Matrix metalloproteinase-9 / Aquaporin-9 / Western blotting / Rat
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