Exploring the method of preparing brain slices for patch clamp recording

CHEN Xing LIU Xiao-dong LI Lin WANG Ke BAI Wen-pei* QIN Li-hua YU Long-chuan*

doi:10.16098/j.issn.0529-1356.2015.01.021

Acta Anatomica Sinica ›› 2015, Vol. 46 ›› Issue (1) : 118-121. DOI: 10.16098/j.issn.0529-1356.2015.01.021

Exploring the method of preparing brain slices for patch clamp recording

CHEN Xing¹LIU Xiao-dong² LI Lin³ WANG Ke⁴ BAI Wen-pei ^1* QIN Li-hua⁴YU Long-chuan ^2*

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Abstract

Objective To explore a method of preparing brain slices for patch clamp technique and to analyze effects of age,solutions and processes of operation on neurons of brain slices. Methods Sixty Sprague-Dawley rats were anesthetized and quickly decapitated. Their brains were cooled and dissected then 300μm coronary brain slices were cut and incubated in the artifical cerebrospinal fluid at 33℃ for 45 minutes. Neurons were observed under an infrared differential aberration interference microscope. Results Neurons showed clear boundary, and three-dimensional appearance and nuclei and particles were not seen. The activity of brain slices depended on various experiment conditions.Conclusion The activity of neurons in the brain slice is associated with age,solutions and processes of operation, thus in experiments we should strictly control these factors.

Key words

Brain slice / Neuron / Patch clamp / Rat

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CHEN Xing LIU Xiao-dong LI Lin WANG Ke BAI Wen-pei* QIN Li-hua YU Long-chuan*. Exploring the method of preparing brain slices for patch clamp recording[J]. Acta Anatomica Sinica. 2015, 46(1): 118-121 https://doi.org/10.16098/j.issn.0529-1356.2015.01.021

References

［1］Moyer JR Jr, Brown TH. Methods for whole-cell recording from visually preselected neurons of perirhinal cortex in brain slices from young and aging rats ［J］. J Neurosci Methods,1998,86(1): 35-54.
［2］Guan BC,Zhang HL, Li ZhW. Basic Principles of Cellular Electrophysiology and Patch Clamp Techniques［M］. Beijing:Science Press,2013:73-75,104-111.(in Chinese)
关兵才,张海林,李之望. 细胞电生理学基本原理与膜片钳技术［M］. 北京：科学出版社, 2013:73-75,104-111.
［3］Marzia Martina, Stefano Taverna. Patch Clamp Methods and Protocols［M］. New York: Humana Press,2014:221-242.
［4］Liu ZhW. Practical Patch Clamp Techniques［M］. Beijing: Military Medical Science Press,2006:170-175. (in Chinese)
刘振伟. 实用膜片钳技术［M］. 北京: 军事医学科学出版社, 2006: 170-175.
［5］Xia J, Kong WJ, Zhu Y, et al. The preparation of vestibular nuclei slices for visual patch clamp techniques［J］. Journal of Audiology and Speech Pathology,2008,16(5):424-429. (in Chinese)
夏交,孔维佳,朱云,等. 可视脑片膜片钳技术前庭核团脑片的制备［J］. 听力学及言语疾病杂志,2008,16(5):424-429.