Welcome to visit Acta Anatomica Sinica! Today is
Chinese
Structure and distribution of extracellular matrix proteins in yak calves testis
YUAN Li-gang* QU Ya-ling ZHU Jun-feng GU Lai-feng TIAN Dan-zeng
Acta Anatomica Sinica ›› 2015, Vol. 46 ›› Issue (1) : 106-112.
Structure and distribution of extracellular matrix proteins in yak calves testis
Objective To characterize the structure and distribution of extracellular matrix proteins of testis in 9 yak calves. Methods Histochemistry and transmission electron methods were used to study the microstructure and ultrastructure of yak calves testis, and immunohistochemical SP technique and Image Pro Plu (IPP) statistics methods were used to identify the distribution of the laminin(LN), type Ⅳ collagen (ColⅣ) and heparan sulfate proteo glycans (HSPG).
Results The germinal epithelium of seminiferous tubule was constructed 1-2 layers by Sertoli cells and gonocytes without appearance of the lumen. The gonocytes were larger and abundant with cytoplasm.Sertoli cells were rich in heterochromatin and the microfilaments constructed by the typical ectoplasmic specialization and actin filament were accumulated in the submembranes regions between the adjacent Sertoli cells.Immunostaining analysis appeared that the extracellular matrix (ECM) proteins LN, ColⅣ and HSPG were weakly present throughout the basement membranes and peritubular myoid cells in yak calves seminiferous tubule.The relative expression of LN in the gonocytes was significantly lower than Col Ⅳ and HSPG（P<0.05）, and no expression in Sertoli cells and leydig cells. The strong immunoreactivity for Col Ⅳ and HSPG was seen in the Sertoli cells. In addition, the distribution of HSPG in the Leydig cells was higher than Col Ⅳ. Conclusion Taken together, the proliferation of the Sertoli cells is the main causes for the development of the yak calves seminiferous tubule and the Leydig cells are in the transition period which developed from embryonal type to a mature stage; the distribution of ECM proteins LN,Col Ⅳand HSPG may serve as a meaningful index for the further research of the yak testis development.
Testis / Collagen / Laminin / Heparan sulfate proteoglycans / Immunohistochemistry / Yak calve
［1］Song Y, Xu ZL. Study on role of the testis extracellular matrix［J］. Acta Anatomica Sinica, 1997, 28(2):221-223.(in Chinese)
宋阳,许增禄.睾丸细胞外间质作用的研究［J］.解剖学报,1997，28(2)：221-223.
［2］Yan P, Pan HP. Environmental effects on yak reproductive performance［J］. Animal Husbandry and Veterinary Medicine, 2004,36(5):15-16.(in Chinese)
阎萍,潘和平. 环境因素对牦牛繁殖性能的影响［J］ 畜牧与兽医, 2004,36(5):15-16.
［3］Guerra AG, Hendrick S, Barth AD. Increase in average testis size of Canadian beef bulls［J］. Can Vet J, 2013,54(5):485-490.
［4］Madrid E1, Reyes JG, Hernández B, et al. Effect of normobaric hypoxia on the testis in a murine model［J］. Andrologia, 2013,45(5):332-338.
［5］Huang FM. The sudy on the mice reproduction and cognitive behavior during exposure to simulated 5000m altitude［D］. Beijing: Chinese Academy of Medical Siences, Peking Union Medical College, 2011: 24-30. (in Chinese)
黄付敏.模拟海拔5000 米高原低氧环境对雄性大小鼠性功能及行为学的影响［D］. 北京:中国医学科学院北京协和医学院, 2011:24-30.
［6］Sun Y. The preliminary study of angioarchitecture of testis and epididymis in Tibetan plateau sheep［D］. Lznzhou:Gansu Agricultural University, 2010:8-14.(in Chinese).
孙英.高原藏羊睾丸及附睾血管构筑的初步研究 ［D］.兰州：甘肃农业大学, 2010:8-14.
［7］Zhang J, Li J, Wang XY, et al. The study of the seminiferous tubule stereology and the expression of PCNA and apoptosis in the rat testes during the postnatal development［J］. Acta Anatomica Sinica, 2002,33(6):617-622. (in Chinese)
张健, 李健, 王晓云等.大鼠生精小管体视学及PCNA、凋亡表达的发育变化研究［J］. 解剖学报, 2002，33(6)：617-622.
［8］Moura AA, Erickson BH. Age-related changes in peripheral hormone concentrations and their relationships with testis size and number of Sertoli and germ cells in yearling beef bulls［J］. J Reprod Fertil, 1997,111(2):183-190.
［9］Li MW, Cheng CY, Mruk DD. Sertolin mediates blood-testis barrier restructuring.［J］.Endocrinology, 2014,155(4):1520-1531.
［10］Zhu LJ, Lu ChY, Zong ShD. Ultrastructure features of the postnatal development of the ectoplasmic specialization in rat sertoli cells［J］. Acta Anatomica,1991,22(3):301-304.(in Chinese)
朱立基，卢春燕，宗书东. 大鼠睾丸Sertoli细胞外质特化结构生后发育的超微结构观察［J］.解剖学报, 1991，22（3）：301-304.〖ZK)〗
［11］Kerr JB,Knell CM,Abbott M,et.al.Ultrastructural analysis of the effect of ethane dimethanesulphonate on the testis of the rat, guinea pig, hamster and mouse［J］.Cell Tissue Res, 1987,249(2):451-457.
［12］Xie Q, Mackay S, Ullmann SL,et al. Testis development in the opossum Monodelphis domestica.［J］.J Anat, 1996,189 (2):393-406.
［13］Ma QF, Hou ShK, Yuan YN, et al. Ultrastructural morphological changes of renal and testis toleration when the organism exposed acutively at altitude hypoxia environment in rabbits［J］. Chinese Journal of Health Care and Medicine, 2009,11(6):420-422. (in Chinese)
马全福,侯世科,袁延年,等.高原低氧应激对兔肾脏和睾丸组织超微结构的影响［J］. 中华保健医学杂志, 2009,11(6):420-422.
［14］Jiang HT, Liang F, Shao ZhX, et al. The regulation of cell junction dynamics in the mammalian testis［J］.Chinese Journal of Cell Biology, 2011,33(4):415-421. (in Chinese)
蒋洪涛，梁芳，邵泞雄, 等.哺乳动物睾丸细胞间连接的动态调控［J］.中国细胞生物学学报, 2011，33(4)：415-421.
［15］Oguzkurt P, Kayaseluk F,Tuncer I, et al. Evaluation of extracellular matrix protein composition in sacs associated with undescended testis, hydrocele, inguinal hernia and peritoneum［J］.Urology, 2007,70(2):346-350.
［16］Virtanen I, Lohi J, Tani T, et al.Distinct changes in the laminin composition of basement membranes in human seminiferous tubules during development and degeneration［J］. Am J Pathol, 1997,150(4):1421-1431.
［17］Berkholtz CB, Lai BE, Woodruff TK, et al. Distribution of extracellular matrix proteins type I collagen, type IV collagen, fibronectin, and laminin in mouse folliculogenesis［J］.Histochem Cell Biol, 2006,126(5):583-592.
［18］Gelly JL, Richoux JP, Leheup BP,et al. Immunolocalization of type IV collagen and laminin during rat gonadal morphogenesis and postnatal development of the testis and epididymis［J］.Histochemistry,1989,93(1):31-37.
［19］Harvey SJ1, Perry J, Zheng K, et al. Sequential expression of type IV collagen networks: testis as a model and relevance to spermatogenesis［J］.Am J Pathol, 2006,168(5):1587-1597.
［20］Jiang DY, Fu XB, Sheng ZhY. The diversity of structure and function of heparin sulfate proteoglycans via modification of some relative enzymes［J］.Chinese Journal of Pathophysiology, 2005, 21(5):1020-1025.(in Chinese)
姜笃银，付小兵，盛志勇.硫酸乙酰肝素糖蛋白的结构功能多样性与相关修饰酶群作用［J］.中国病理生理杂志，2005，21(5)：1020-1025.
/
| 〈 |
|
〉 |
