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Influence of 5-hydroxytryptamine on stress-induced diarrhea of weaning mice
YANG Chen-yu HAN Ya-nan WANG Zi-xu CHEN Yao-xing QIN Zhuo-ming CAO Jing SONG Jin-yuan DONG Yu-lan*
Acta Anatomica Sinica ›› 2015, Vol. 46 ›› Issue (1) : 94-100.
Influence of 5-hydroxytryptamine on stress-induced diarrhea of weaning mice
Objective To investigate the relationship between stress-induced diarrhea and 5-hydroxytryptamine (5-HT) and explore the possible mechanism of stress-induced diarrhea. Methods Citalopram hydrobromide (CH) was used to increase 5-HT content and para-chlorophenylalanine (PCPA) was used to decrease 5-HT intracellular synthesis. Seventy-two male 21 days newly weaned ICR mice were randomly divided into six groups: control, CH group, PCPA group, stress diarrhea group, CH+stress diarrhea group, PCPA+stress diarrhea group. Daily administration of intraperitoneal injection of CH 10 mg/kg or PCPA 300mg/kg, 4 hours later, the last three groups were administrated with intragastric of senna (0.4 kg/L) with 15ml/kg BW dose and hind legs binding stress for 4 hours. The control group was administrated with equal relative doses of saline. Five days later detected blood sugar levels of animals were detected, ELISA and immunohistochemical staining was used to measure 5-HT and corticosterone (Cort) content. Results The data of stress diarrhea and CH treated mice showed an increase in diarrhea score, blood sugar levels and Cort in plasma, but a significant decrease in weight gain coincided with an increase in 5-HT in plasma and intestine compared to that of control animals. Stress diarrhea mice following CH administration showed significantly raised 5-HT in plasma and intestine, which was in coincided with the increased diarrhea score but the greatly decreased weight gain of animals when compared to stress diarrhea mice. Administration of PCPA to mice did not change significantly weight gain and blood sugar levels, but increased Cort content compared to control animals. While treatment with PCPA to stress diarrhea mice resulted in a significantly increase in weight gain and decrease in blood sugar levels, 5-HT, and Cort content compared to stress diarrhea mice, but still was higher than that of control animals. Conclusion 5-HT content in stress diarrhea mice significantly increased, in turn, CH-induced increase of 5-HT content directly resulted in and aggravated stress-induced diarrhea of weaning mice. Conversely, reducing 5-HT content induced by PCPA weakened degree of diarrhea.
Stress diarrhea / 5-Hydroxytryptamine / Citalopram hydrobromide / Para-chlorophenylalanine / Immunohistochemistry / Newly weaned mouse
［1］Miyata K, Kamato T, Nishida A, et al. Role of the serotonin3 receptor in stress-induced defecation ［J］. J Pharmacol Exp Ther, 1992, 261(1): 297 -303.
［2］Kato S. Role of serotonin 5-HT3 receptors in intestinal inflammation［J］. Biol Pharm Bull, 2013, 36(9):1406-1409.
［3］Gershon MD, Tack J. The serotonin signaling system: from basic understanding to drug development for functional GI disorders［J］. Gastroenterology, 2007, 132(1):397-414.［4］Li D, Lü Y, Tang F. Inducing of irritable bowel Syndrome with diarrhea in rats［J］. Tianjin Journal of Traditional Chinese Medicine, 2009, 3(26): 240-242. (in Chinese)
李丹，吕妍，唐方. 腹泻型肠易激综合征大鼠模型的建立［J］. 天津中医药，2009，3（26）：240-242.
［5］Xu HZh, Xie JQ, Shi B, et al. Expression of inflammatory cytokine in colon mucosa of D-IBS rats and effects of “Wenzhong Jianpi Decoction” on them［J］. Shanghai Journal of Traditional Chinese Medicine, 2007, 6(41): 69-72. (in Chinese)
徐海珍，谢建群，施斌，等. 腹泻型肠易激综合征大鼠结肠黏膜炎性细胞因子的表达及温中健脾方对其影响的研究［J］. 上海中医药杂志，2007，6（41）：69-72.
［6］Deng ZhＨ, Xu ChD, Chen ShＮ. Establish rotavirus diarrhea model in neonatal mice and investigate the pathological pathogenesis［J］. Journal of Clinical Pediatrics，2008, 10(26):862-865. (in Chinese)
邓朝晖，许春娣，陈舜年.新生小鼠轮状病毒腹泻模型建立及病理学致病机制研究［J］. 临床儿科杂志， 2008，10（26）：862-865.
［7］Xie ZhH, Xiao RＦ, Peng ZhＰ, et al. Effect of Qinxiang Zhixie decoction on iNOS mRNA expression human rotavirus diarrhea in neonatal rat［J］. Chinese Traditional Patent Medicine，2013, 4(35): 647-651. (in Chinese)
谢朝晖，肖瑞飞，彭芝配，等. 秦香止泻汤对人轮状病毒腹泻乳鼠诱导型一氧化氮合酶 mRNA 表达的影响［J］. 中成药，2013，4（35）：647-651.
［8］Xie JQ, Li GX, Lu X, et al. Experimental study on serum 5-HT of irritable diarrhea mice treated with “liver -soothing and spleen-strengthening decoction”［J］. Acta Universitatis Traditionis Medicalis Sinensis Pharmacologiaeque Shanghai, 1999, 13(2): 46-48. (in Chinese)
谢建群，李国霞，陆雄，等. 疏肝健脾汤对应激腹泻小鼠血清5-HT的实验研究［J］. 上海中医药大学学报，1999，13（2）：46-48.
［9］Shi Q, Zhao Y, Mei ZhS, et al. Expression of 5-HT and MT in IBS-D rats caused by maternal separation［J］. Military Medical Sciences, 2013, 37(8): 604-608. (in Chinese)
石卿，赵云，梅竹松，等. 5-羟色胺与褪黑激素在母婴分离致腹泻型肠易激综合征模型大鼠中的表达［J］. 军事医学，2013，37（8）：604-608.
［10］Kawakami N, Araki S, Takatsuka N, et al. Overtime, psychosocial working conditions, and occurrence of non-insulin dependent diabetes mellitus in Japanese men［J］. J Epidemiol Community Health, 1999, 53(6): 359-563.
［11］Zhang X, Song H, Chen N, et al. Relationship between occupational shand blood glucose, bloodfipid, bloodpressure of video display terminnl operators［J］. Chinese Journal of Industrial Hygiene and Occupational Diseases, 2007, 25(03): 142-144. (in Chinese)
张星，宋辉，陈楠，等. 视屏作业人员职业应激与血糖和血脂及血压的关系［J］. 中华劳动卫生职业病杂志，2007，25（03）：142-144.
［12］Smith F, Clark JE, Overman BL, et al. Early weaning stress impairs development of mucosal barrier function in the porcine intestine［J］. Am J Physiol Gastrointest Liver Physiol, 2010, 298(3): 352-363.
［13］Meddings JB, Swain MG. Environmental stress-induced gastrointestinal permeability is mediated by endogenous glucocorticoids in the rat［J］. Gastroenterology, 2000, 119(4): 1019-1028.
［14］Qing XH, Li XF, Zhao QY, et al. Animal diarrhea resulted from stress and changes of 5-hydroxytryptamine levels in serum of the animal［J］. Chinese Journal of Veterinary Science and Technology, 2003, 33(3): 57-59. (in Chinese)
卿晓红，李先方，赵全跃，等. 应激所致动物腹泻及动物血清5-羟色胺含量的变化［J］. 中国兽医科技，2003，33（3）：57-59.
［15］Cong J, Cai G, Zhang ZhL, et al. Effects of Changjitai decoction on the changes of colon mast cells and the expression of 5-hydroxytryptamine in rats with irritable bowel syndrome of diarrhea type［J］. Journal of Anhui Traditional Chinese Medical College, 2010, 6(29): 47-51. (in Chinese)
从军，蔡淦，张正利，等. 肠吉泰对腹泻型肠易激综合征大鼠结肠肥大细胞变化和5-羟色胺表达的影响［J］. 安徽中医学院学报，2010，6（29）：47-51.
［16］Bertrand PP, Bertrand RL. Serotonin release and uptake in the gastrointestinal tract［J］. Auton Neurosci, 2010, 153(1-2): 47-57.
［17］Bertrand PP, Hu X, Mach J, et al. Serotonin (5-HT) release and uptake measured by real-time electrochemical techniques in the rat ileum［J］. Am J Physiol Gastrointest Liver Physiol, 2008, 295 (6): 1228-1236.
［18］Bertran PP, Barajas-Espinosa A, Neshat S, et al.Analysis of real-time serotonin (5-HT) availability during experimental colitis in mouse［J］. Am J Physiol Gastrointest Liver Physiol, 2010, 298(3):446-455.
［19］Tharayil VS, Wouters MM, Stanich JE, et al. Lack of serotonin 5-HT2B receptor alters proliferation and network volume of interstitial cells of Cajal in vivo［J］. Neurogastroenterol Motil, 2010, 22(4): 462-479.
［20］Liu L, Sun HM, Wu B, et al. Morphology of interstitial cells of Cajal in the gastrointestinal tract of Mongolian gerbil［J］. Acta Anatomica Sinica, 2013, 1(44): 93-96. (in Chinese)刘丽，孙海梅，吴波，等. 长爪沙鼠胃肠道Cajal间质细胞的形态学［J］. 解剖学报，2013，1（44)：93-96.
［21］Yadav VK, Ryu JH, Suda N, et al. Lrp5 controls bone formation by inhibiting serotonin synthesis in the duodenum［J］. Cell, 2008, 135(5): 825-837.
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