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Anti-tumor effect and mechanism of cyclooxygenase 2 inhibitor in pancreatic cancer
GU Zhuo-yu LI Jun1* LI Si-yuan* XIAO Zhi-wei ZHOU Ting
Acta Anatomica Sinica ›› 2015, Vol. 46 ›› Issue (1) : 81-84.
Anti-tumor effect and mechanism of cyclooxygenase 2 inhibitor in pancreatic cancer
Objective To investigate the effect of COX-2 inhibitor on proliferation, migration, invasion and the expression of COX-2, matrix metalloproteinase (MMP)-14 in pancreatic cancer cell and its possible anti-tumor mechanism. Methods Human pancreatic cancer cell line PANC-1 was treated with diverse concentrations of COX-2 inhibitor celebrex (20, 60, 100μmol/L). Cell proliferation, invasion and migration capabilities were measured by MTT colorimetry, Transwell invasion assay, and scratch assay. The protein expression of COX-2 and MMP-14 was assessed by ELISA. Results The proliferation, invasion and migration capabilities were decreased in a concentration-dependent manner by COX-2 inhibitor (P<0.05). The protein expression of COX-2 and MMP-14 was correspondingly reduced (P<0.05). MMP-14 expression was positively correlated with COX-2 expression (r=0.873，P<0.01). Conclusion COX-2 inhibitor may down-regulate MMP-14 expression via inhibiting COX-2 expression, then attenuating the proliferation, invasion and migration of human pancreatic cancer cell in a concentration-dependent manner, contributing to its anti-tumor effect in pancreatic cancer.
Pancreatic cancer / Cyclooxygenase 2 inhibitor / Matrix metalloproteinase 14 / Invasion / Migration / Methyl thiazolyl tetrazolium method / Human
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