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Resveratrol inhibits lipopolysaccharide-induced activation of osteoclast precursor Raw 264.7 cells
ZONG Yi CHEN Jin GUO Jia-zhi ZHANG Xiu-jun ZHANG Tie-jun SUN Lin*
Acta Anatomica Sinica ›› 2015, Vol. 46 ›› Issue (1) : 51-56.
Resveratrol inhibits lipopolysaccharide-induced activation of osteoclast precursor Raw 264.7 cells
Objective To investigate the inhibiting effects of resveratrol (Res) on the expression of potentially inflammatory cytokines by cultured osteoclast precursor Raw 264.7 cells stimulated with lipopolysaccharide (LPS). Methods Inflammatory cell model was established by LPS-stimulated Raw 264.7 cells. The Raw 264.7 cell identification test was measured by tartrate resistant acid phosphatase (TRAP) staining. The cells were treated with Res (1 μmol/L and 5 μmol/L) prior to LPS (5 mg/L) exposure. The effects on the mRNA and protein levels of inflammatory enzymes, inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2), and inflammatory cytokines, tumor necrosis factor-α (TNF-α), and interleukin-1β (IL-1β), inflammatory signaling proteins nuclear factor-κB (NF-κB) were analysed by reverse transcription-polymerase chain reaction (RT-PCR) and double-immunofluorescence labeling assay. The effects of Res on cytotoxicity determination of Raw 264.7 cells were measured by MTT assay. Results LPS-induced iNOS, COX-2 and NF-κB protein and mRNA expression levels were significantly decreased by Res. Res had an effect on the expression of TNF-α, IL-1β through transcriptional and translational inhibition. Conclusion The inhibitory effects of Res on LPS-induced inflammatory mediators in osteoclast precursor cells exert functions on its anti-osteoporosis through NF-κB.
Resveratrol / Lipopolysaccharide / Raw 264.7 cells / Inflammatory cytokines / RT-PCR / Double-immunofluorescence labeling / Mouselabeling
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