Welcome to visit Acta Anatomica Sinica! Today is
Chinese
Neuroprotective effect of grafting thyroid hormone-induced neural stem cells on chronic experimental allergic encephalomyelitis
DU Jie HU Guang-qiang DENG Li YANG Chao-xian GUO Kan GAO Xiao-qing*
Acta Anatomica Sinica ›› 2015, Vol. 46 ›› Issue (1) : 13-19.
Neuroprotective effect of grafting thyroid hormone-induced neural stem cells on chronic experimental allergic encephalomyelitis
Objective To explore whether transplantation of neural stem cells induced by thyroid hormone (T3) provides a better neuroprotective effect than native NSCs after chronic experimental allergic encephalomyelitis. Methods Neural stem cells (NSCs) and T3-induced NSCs of neonatal rat were cultured in vitro. After 7 days they were induced to differentiate, immunohistochemistry or immunofluorescence staining were used to detect GalC+ and GFAP+ cells respectively. Chronic EAE rat models were induced by Guinea pig spinal cord homogenate, fo11owed by infusion of T3/NSCs, NSCs (T3/NSCs and NSCs were labeled with 5-bromo-2’-deoxyuridine (BrdU) and saline (T3/NSCs, NSCs and control groups，respectively) at 10 days after EAE immunization, and each group had 10 rats. Clinical scores for every day were performed to evaluate neuro1ogica1 function. All rats were sacrificed at 60d after EAE immunization. HE and LFB staining was used to observe inflammatory infiltrates and demyelination in brain respectively. Immunofluorescent double staining was used to test the ratio of GalC+/BrdU+ and GFAP+/BrdU+in brain. RT-PCR assay was used to identify the expression of mRNA for PDGFαR, GalC and MBP of brain tissue. Results The ratio of GalC+ or GFAP+ cells of T3/NSCs was respectively higher or lower than that of NSCs in vitro. Significant recovery of neuro1ogica1 function was found in T3/NSCs and NSCs groups compared with control group. Improvement of inflammatory infiltrates and demyelination in T3/NSCs groups was stronger than that in NSCs groups. In brain, the ratio of GalC+/BrdU+ or GFAP+/BrdU+ in T3/NSCs group was respectively higher or lower than that in NSCs group. And the expression of mRNA for PDGFαR, GalC and MBP of brain tissue in T3/NSCs groups was stronger than in NSCs groups. Conclusion The results demonstrate that grafting T3-induced NSCs provides better neuroprotection for EAE than grafting NSCs alone.
Thyroid hormone / Neural stem cell / Cell transplantation / Allergy / Encephalomyelitis / Immunofluorescence / Rat
［1］Fernandez M, Giuliani A, Pirondi S, et al. Thyroid hormone administration enhances remyelination in chronic demyelinating inflammatory disease［J］. PNAS, 2004, 101(46): 16363-16368.［2］Einstein O, Grigoriadis N, Mizrachi-Kol R, et al. Transplanted neural precursor cells reduce brain inflammation to attenuate chronic experimental autoimmune encephalomyelitis［J］. Exp Neurol, 2006, 198(2): 275-284.
［3］Fazeli AS, Nasrabadi D, Pouya A, et al. Proteome analysis of post-transplantation recovery mechanisms of an EAE model of multiple sclerosis treated with embryonic stem cell-derived neural precursors［J］. J Proteomics. 2013, 94: 437-450.
［4］Hackett C, Knight J, Mao-Draayer Y. Transplantation of Fas-deficient or wild-type neural stem/progenitor cells (NPCs) is equally efficient in treatingexperimental autoimmune encephalomyelitis (EAE)［J］. Am J Transl Res, 2014, 6(2): 119-128.
［5］Kassis I, Grigoriadis N, Gowda-Kurkalli B, et al. Neuroprotection and immunomodulation with mesenchymal stem cells in chronic experimental autoimmune encephalomyelitis［J］. Arch Neurol, 2008, 65(6): 753-761.
［6］Kassis I, Petrou P, Halimi M, et al. Mesenchymal stem cells (MSC) derived from mice with experimental autoimmune encephalomyelitis (EAE) suppress EAE and have similar biological properties with MSC from healthy donors［J］. Immunol Lett, 2013, 154(1-2): 70-76.
［7］Liu R, Zhang Z, Lu Z,et al. Human umbilical cord stem cells ameliorate experimental autoimmune encephalomyelitis by regulating immunoinflammation and remyelination［J］. Stem Cells Dev, 2013, 22(7): 1053-1062.
［8］Ghasemi N, Razavi S, Mardani M, et al. Transplantation of human adipose-derived stem cells enhances remyelination in lysolecithin-induced focal demyelination of rat spinal cord［J］. Mol Biotechnol, 2014, 56(5): 470-478.
［9］Fernández M, Paradisi M, Del Vecchio G, et al. Thyroid hormone induces glial lineage of primary neurospheres derived from non-pathological and pathological rat brain: implications for remyelination-enhancing therapies［J］. Int J Dev Neurosci, 2009, 27(8): 769-778.
［10］Kono DH, Urban JL，Horvath SJ, et al. Two minor determinants of myelin basic protein induce experimental allergic encephalomyelitis in SJL/J mice［J］. J Exp Med, 1988, 168(1): 213-227.
［11］Moore S, Khalaj AJ, Yoon J, et al. Therapeutic laquinimod treatment decreases inflammation, initiates axon remyelination, and improves motor deficit in a mouse model of multiple sclerosis［J］. Brain Behav, 2013, 3(6): 664-682.
［12］Calza L, Fernandez M, Giuliani A, et al. Thyroid hormone activates oligodendrocyte precursors and increases a myelin-forming protein and NGF content in the spinal cord during experimental allergic encephalomyelitis［J］. PNAS, 2002, 99(5): 3258-3263.
［13］Dell'Acqua ML, Lorenzini L, D'Intino G, et al. Functional and molecular evidence of myelin- and neuroprotection by thyroid hormone administration in experimental allergic encephalomyelitis［J］. Neuropathol Appl Neurobiol, 2012, 38(5): 454-470.
［14］Dugas JC, Ibrahim A, Barres BA. The T3-induced gene KLF9 regulates oligodendrocyte differentiation and myelin regeneration［J］. Mol Cell Neurosci, 2012, 50(1): 45-57.
［15］Pluchino S, Quattrini A, Brambilla E, et al. Injection of adult neurospheres induces recovery in a chronic model of multiple sclerosis［J］. Nature, 2003, 422 (6933): 688-694.
［16］Nessler J, Bénardais K, Gudi V, et al. Effects of murine and human bone marrow-derived mesenchymal stem cells on cuprizone induced demyelination［J］. PLoS One, 2013, 8(7): e69795.
［17］Proescholdt MA, Jacobson S, Tresser N, et al. Vascular endothelial growth factor is expressed in multiple sclerosis plaques and can induce inflammatory lesions in experimental allergic encephalomyelitis rats［J］. J Neuropathol Exp Neurol, 2002, 61(10): 914-925.
［18］Zappia E, Casazza S, Pedemonte E, et al. Mesenchymal stem cells ameliorate experimental autoimmune encephalomyelitis inducing T-cell anergy［J］. Blood, 2005, 106(5): 1755-1761.
［19］Liu B, Li LY, Liu ChR, et al. Effects of T3 on differentiation of human neural stem cells to oligodendrocyte［J］. Acta Anatomica Sinica, 2003, 34(2): 213-216.
刘奔, 李兰英, 刘春蓉, 等. T3对人神经干细胞分化为少突胶质细胞的影响［J］. 解剖学报，2003, 34(2): 213-216. (in Chinese)
［20］Li R, Xu W, Chen Y, et al. Raloxifene suppresses experimental autoimmune encephalomyelitis and NF-kb-dependent CCL20 expression in reactive astrocytes［J］. Plos One, 2014, 9 (4): 1-10.
/
| 〈 |
|
〉 |
