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Neural differentiation and synapse formation in mouse induced pluripotent stem cells
FAN Wen-juan CHEN Xu-dong YUAN Lei RAO Shu-mei WANG Fu-qing* DENG Jin-bo*
Acta Anatomica Sinica ›› 2015, Vol. 46 ›› Issue (1) : 6-12.
Neural differentiation and synapse formation in mouse induced pluripotent stem cells
Objective To observe whether mouse induced pluripotent stem cells (iPSCs) efficiently can differentiate to functional neurons and formation synapse in vitro. Methods Mouse iPSCs were pre-differentiated into neural stem cells by using retinoic acid (RA) after embryoid body (EB) formation. After RA removed, immunofluorescence staining was used to study the synaptogenesis between neurons，and FM1-43 staining was used to show synaptic terminal with functional activity. Results Neural precursors matured faster, differentiated to functional neurons that stained positively for mature neuronal and glial markers under adherent culture, and iPSCs-derived neurons formed dendritic spines and synaptic connections by morphological analyses. Under depolarization, the activity of synapsis was enhanced and a large number of FM1-43 endocytosis particles were in axon terminal. Conclusion Our results reveal that the processes involved in the formation of synapses in mouse iPSCs differentiate into functional neurons and glia, which may have important implications for neurodevelopmental studies, safety pharmacological studies, and autologous cell transplantation.
Induced pluripotent stem cell / Neural differentiation / Synapse formation / Immunofluorescence / Mouse
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