Effect of interleukin-6 on nucleated erythrocytes in lipopolysaccharide
induced preeclampsia rats

LIU Li-Ping; XU Hui; ZHU Meng-Ni

doi:10.16098/j.issn.052-1356.2023.06.015

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Acta Anatomica Sinica ›› 2023, Vol. 54 ›› Issue (6) : 722-729. DOI: 10.16098/j.issn.052-1356.2023.06.015

Histology,Embryology and Developmental Biology

Effect of interleukin-6 on nucleated erythrocytes in lipopolysaccharide induced preeclampsia rats

LIU Li-ping¹ ZHU Meng-ni¹ XU Hui^2*

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Abstract

Objective To explore the effect of interleukin (IL)-6 on nucleated erythrocytes in lipopolysaccharide (LPS)-induced preeclampsia rats. Methods ELISA and immunohistochemistry were used to detect the IL-6 in peripheral blood and placenta of preeclampsia and normal pregnancy; Flow cytometry and immunofluorescence were used to detect the maternal nucleated erythrocytes. Pregnant SD rats were randomly divided into 3 groups: the control, LPS and LPS +anti-IL-6 group; IL-6, the proportion of nucleated erythrocytes, JAK2/MEK and PI3K/Akt signal-related genes were detected. Results The IL-6 of preeclampsia was higher than that of normal patients. Compared with the Control group, IL-6, the proportion of nucleated erythrocytes and JAK2, P85, Akt, P65, IL-1B mRNA of LPS group increased, the fetal weight decreased; Compared with the LPS group, IL-6, the proportion of nucleated erythrocytes and JAK2, P85, Akt, P65 and IL-1B mRNA of the LPS+anti-IL-6 group decreased. Conclusion The up-regulation of IL-6 of preeclampsia patients is accompanied by increased nucleated erythrocytes in peripheral blood. Neutralizing IL-6 in vivo may down-regulate JAK2/PI3K/Akt/NF-κB-signal-mediated IL-1B to protect preeclampsia rats.

Key words

Preeclampsia / Interleukin 6 / Nucleated erythrocyte / Interleukin 1B / Immunohistochemistry / Immunofluorescence / Human / Rat

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LIU Li-ping ZHU Meng-ni XU Hui.

Effect of interleukin-6 on nucleated erythrocytes in lipopolysaccharide induced preeclampsia rats

[J]. Acta Anatomica Sinica. 2023, 54(6): 722-729 https://doi.org/10.16098/j.issn.052-1356.2023.06.015

References

［1］Ives CW, Sinkey R, Rajapreyar I, et al. Preeclampsia-pathophysiology and clinical presentations: JACC state-of-the-art review［J］. J Am Coll Cardiol，2020, 76(14): 1690-1702.

［2］Yang Z, Zhang WY. Interpretation of the "Guidelines for the Diagnosis and Treatment of Pregnancy Induced Hypertension (2020)" ［J］. Chinese Journal of Obstetrics and Gynecology, 2020, 55(6): 425-432 (in Chinese)

杨孜，张为远. 《妊娠期高血压疾病诊治指南(2020)》解读［J］. 中华妇产科杂志, 2020, 55(6): 425-432.

［3］Cui L, Takada H, Takimoto T, et al. Immunoregulatory function of neonatal nucleated red blood cells in humans［J］. Immunobiology, 2016, 221(8): 853-861.

［4］Hu G, Guan R, Li L. Nucleated red blood cell count in maternal peripheral blood and hypertensive disorders in pregnant women［J］. Am J Med Sci, 2016, 351(2): 140-146.

［5］Aggarwal R, Jain A K, Mittal P, et al. Association of pro-and anti-inflammatory cytokines in preeclampsia［J］. J Clin Lab Anal, 2019, 33(4): e22834.

［6］Wang G J, Yang Z, Huai J, et al. Pravastatin alleviates oxidative stress and decreases placental trophoblastic cell apoptosis through IL-6/STAT3 signaling pathway in preeclampsia rats［J］. Eur Rev Med Pharmacol Sci, 2020, 24(24): 12955-12962.

［7］Ding H, Dai Y, Lei Y, et al. Upregulation of CD81 in trophoblasts induces an imbalance of Treg/Th17 cells by promoting IL-6 expression in preeclampsia［J］. Cell Mol Immunol, 2019, 16(1): 302-312.

［8］Ferber A, Minior V K, Bornstein E, et al. Fetal "nonreassuring status" is associated with elevation of nucleated red blood cell counts and interleukin-6［J］. Am J Obstet Gynecol, 2005, 192(5): 1427-1429.

［9］Hu G, Guan R, Li L. Nucleated red blood cell count in maternal peripheral blood and hypertensive disorders in pregnant women［J］. Am J Med Sci, 2016, 351(2): 140-146.

［10］Hebbar S, Misha M, Rai L. Significance of maternal and cord blood nucleated red blood cell count in pregnancies complicated by preeclampsia［J］. J Pregnancy, 2014, 2014: 496416.

［11］Pitman H, Innes BA, Robson SC, et al. Altered expression of interleukin-6, interleukin-8 and their receptors in decidua of women with sporadic miscarriage［J］. Hum Reprod, 2013, 28(8): 2075-2086.

［12］Saggini A, Chimenti S, Chiricozzi A. IL-6 as a druggable target in psoriasis: focus on pustular variants［J］. J Immunol Res, 2014, 2014: 964069.

［13］Ibrahim MH, Moustafa AN, Saedii A, et al. Cord blood erythropoietin and cord blood nucleated red blood cells for prediction of adverse neonatal outcome associated with maternal obesity in term pregnancy: prospective cohort study［J］. J Matern Fetal Neonatal Med, 2017,30(18):2237-2242.

［14］Pitman H, Innes BA, Robson SC, et al. Altered expression of interleukin-6, interleukin-8 and their receptors in decidua of women with sporadic miscarriage［J］. Hum Reprod, 2013,28(8):2075-2086.

［15］Hebbar S, Misha M, Rai L. Significance of maternal and cord blood nucleated red blood cell count in pregnancies complicated by preeclampsia［J］. J Pregnancy, 2014,2014:496416.

［16］Wang H, Wang Z, Wang L, et al. IL-6 promotes collagen-induced arthritis by activating the NLRP3 inflammasome through the cathepsin B/S100A9-mediated pathway［J］. Int Immunopharmacol, 2020,88:106985.