幼年特发性关节炎患儿关节滑膜组织中趋化因子CXCL10及其受体CXCR3的表达及意义

宫相翠

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解剖学报 ›› 2017, Vol. 48 ›› Issue (5) : 590-594.

组织学胚胎学发育生物学

幼年特发性关节炎患儿关节滑膜组织中趋化因子CXCL10及其受体CXCR3的表达及意义

宫相翠^1* 管慧² 刘杰1

作者信息 +

Significance of CXCL10/ CXCR3 expression in the synovium of children with juvenile idiopathic arthritis

GONG Xiang-cui^1* GUAN Hui² LIU Jie¹

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文章历史 +

摘要

目的检测趋化因子CXCL10 及其受体CXCＲ3 在幼年特发性关节炎( JIA) 患儿关节滑膜组织中的表达，探讨其在JIA 发病机制中的作用。方法通过免疫组织化学方法，检测12 例JIA 患儿和4 例非JIA 患儿关节滑膜组织中CXCL10 和CXCＲ3 的表达; 通过半定量ＲT-PCＲ方法，检测CXCＲ3 在JIA 患儿和对照幼儿关节滑膜组织中mＲNA 水平的表达。结果免疫组织化学结果表明，CXCL10 / CXCＲ3 在JIA 患儿及对照组阳性表达差异有显著性( P ＜ 0. 05) ; CXCＲ3 在JIA 患儿关节滑膜组织中mＲNA 表达水平( CXCＲ3∶ GAPDH 为2. 26 ± 1. 55) 显著高于对照组( 0. 66 ± 0. 44) ，两者差异有显著性( P ＜ 0. 05) 。结论趋化因子CXCL10 及其受体CXCＲ3 在幼年特发性关节炎( JIA) 的发病中可能起到了重要的作用。

Abstract

Objective To investigate the expression of chemokine CXCL10 and its receptor CXCＲ3 in synovium of children with juvenile idiopathic arthritis ( JIA) and to explore the role of CXCL10 and CXCＲ3 in the pathogenesis of JIA． Methods Immunohistochemical method was used to detect CXCL10 / CXCＲ3 expression in the synovium of 12 cases of children with JIA and 4 cases of non JIA children． Semi quantitative ＲT-PCＲ was used to detect CXCＲ3 mＲNA expression in children with JIA and control．Ｒesults There were significant differences in CXCL10 / CXCＲ3 expression between children with JIA and control group ( P ＜ 0. 05) ． CXCＲ3 mＲNA expression in children with JIA ( CXCＲ3 ∶ GAPDH 2. 26 ± 1. 55) was significantly higher than that in the control group ( 0. 66 ± 0. 44，P ＜ 0. 05) ． Conclusion CXCL10 and CXCＲ3 may play important roles in the pathogenesis of juvenile idiopathic arthritis ( JIA) ．

导出引用

宫相翠管慧刘杰. 幼年特发性关节炎患儿关节滑膜组织中趋化因子CXCL10及其受体CXCR3的表达及意义[J]. 解剖学报. 2017, 48(5): 590-594

GONG Xiang-cui GUAN Hui LIU Jie. Significance of CXCL10/ CXCR3 expression in the synovium of children with juvenile idiopathic arthritis[J]. Acta Anatomica Sinica. 2017, 48(5): 590-594

中图分类号： Ｒ322. 81

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