慢性寒冷暴露与小鼠心肌及脑血管的改变

屈婷婷; 李瑞玲; 王颖; 周莉; 邓锦波; 邓洁心

解剖学报 ›› 2016, Vol. 47 ›› Issue (1) : 80-86.

组织学胚胎学发育生物学

慢性寒冷暴露与小鼠心肌及脑血管的改变

屈婷婷¹,李瑞玲²,王颖²,周莉³,邓锦波⁴,邓洁心⁵

作者信息 +

The alterations of heart and cerebral vasculature in mice after chronic cold exposure

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文章历史 +

摘要

[摘要] 目的探讨慢性寒冷暴露对小鼠心脏及脑血管的影响，及寒冷诱导心脑血管疾病的相关机制。方法选择生后90～180d的成年雄性小鼠60只，随机分为正常对照组和寒冷暴露组两组，每组30只。寒冷暴露组置于温度为-1―4℃环境中饲养30d，建立慢性寒冷暴露模型。测量心脏指数(heart mass index, HMI)；采用血细胞分析仪检测血常规参数；化学发光法检测血清雌激素水平；HE染色法观察心肌细胞形态；墨汁灌注法观察脑血管体密度。结果慢性寒冷暴露可诱导小鼠心肌肥厚，脑血管体密度减少，白细胞、淋巴细胞、红细胞和血小板数目明显增多，对心脏和脑血管造成损伤。结论慢性寒冷暴露可导致心脏和脑血管的结构和功能改变，这与雌激素直接参与寒冷应激反应过程密切相关。

Abstract

[Abstract] Objective: The study was tried to investigate the alterations of heart and cerebral vessels after chronic cold exposure in mice. In the meantime, the pathological mechanisms were discussed as well. Methods: Male mice of ages from 90 to 180 d were used, and 60 mice were divided into control group and cold exposure group in random. Cold exposure group was fed at temperature from -1℃ and 4 ℃ for 30 days to make the chronic cold exposure model. Then, heart mass index was measured. Blood routine analyses were detected using blood cell analyzer, and the serum estrogen levels were detected by chemiluminescence method. The histological structure of cardiomyocytes was visualized with hematoxylin-eosin（H. E.）staining, and the cerebral vasculature was visualized with ink perfusion assay. Results: Chronic cold exposure could induce mouse’s myocardial hypertrophy and decrease the cerebral vascular density. The number of white blood cells, lymphocytes, red blood cells and blood platelet increased, and estrogen level of blood increased as well, which contributed to the pathological alterations of cardiovascular system. Conclusion: Chronic cold exposure could induce myocardial hypertrophy and the alterations of blood cells, cerebral vasculature and estrogen. The pathological alterations of blood cells, cerebral vasculature and estrogen are involved in the production of myocardial hypertrophy.

导出引用

屈婷婷李瑞玲王颖周莉邓锦波邓洁心. 慢性寒冷暴露与小鼠心肌及脑血管的改变[J]. 解剖学报. 2016, 47(1): 80-86

The alterations of heart and cerebral vasculature in mice after chronic cold exposure[J]. Acta Anatomica Sinica. 2016, 47(1): 80-86

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