鸡肠神经胶质细胞超微结构特征及其标记蛋白钙结合蛋白S100β在小肠中的分布

徐春生; 祁凤华; 摆世林; 陈城; 蔡婷婷

解剖学报 ›› 2016, Vol. 47 ›› Issue (1) : 7-11.

神经生物学

鸡肠神经胶质细胞超微结构特征及其标记蛋白钙结合蛋白S100β在小肠中的分布

徐春生,祁凤华,摆世林,陈城,蔡婷婷

作者信息 +

Ultrastructural features of enteric glial cells and the distribution of S-100 beta in the small intestine of chicken

Author information +

文章历史 +

摘要

目的观察了10只健康黄羽肉鸡肠神经胶质细胞结构特征及S-100β蛋白的分布特点。方法采用透射电镜技术，观察神经胶质细胞的超微结构特点，免疫组织化学SABC-AP法研究S-100β蛋白的分布特征。结果电镜观察表明鸡肠神经胶质细胞（EGCs）在小肠各段均呈星形，细胞核不规则，胞质中分布有大小不一的圆形或椭圆形无髓神经纤维。免疫组织化学显示，S-100β在鸡小肠各段黏膜上皮细胞、肠腺表达较强，其中，上皮细胞基膜和肠腺上皮细胞顶端为强阳性，固有膜表达较弱，在黏膜下神经丛和肌间神经丛均呈强阳性表达。结论鸡肠神经胶质细胞超微结构特征及其在小肠的分布特点与哺乳动物较为相似。

Abstract

objective To observe the structure of Enteric glial cell and distribution of S-100β protein in 10 healthy yellow feather broiler chickens. Methods The ultrastructural characteristics of glial cells were observed by transmission electron microscopy， and the distribution of S-100 protein was studied by immunohistochemical SABC-AP method. Results The electron microscope showed that chicken enteric glial cells (EGCS) in the small intestine were star， irregular nucleus， cytoplasm distribution has a small round or oval unmyelinated nerve fibers. Immunohistochemistry showed that in chicken intestine epithelium mucosa of each segment， intestinal glands of S-100βwere expressed strong. Among the basement membrane of epithelial cells and at the top of intestinal gland epithelial cells were the strong positive， lamina propria weaker expression， in the submucosal plexus and myenteric plexus showed strong positive expression. Conclusion the glial cell ultrastructure characteristics and distribution characteristics of S-100βare similar to the small intestine of mammals.

导出引用

徐春生祁凤华摆世林陈城蔡婷婷. 鸡肠神经胶质细胞超微结构特征及其标记蛋白钙结合蛋白S100β在小肠中的分布[J]. 解剖学报. 2016, 47(1): 7-11

Ultrastructural features of enteric glial cells and the distribution of S-100 beta in the small intestine of chicken[J]. Acta Anatomica Sinica. 2016, 47(1): 7-11

参考文献

[1]Alexei Verkhratsky，Arthur Morgan Butt.Glial neurobiology[M].England: John Wiley & Sons Ltd，2007:39-80. [2]Liu YA， Chung YC， Pan ST， Shen MY， Hou YC，Peng SJ， Pasricha PJ， Tang SC.3-D imaging，illustration，and quantitation of enteric glial network in transparent human colon mucosa[J]. Neurogastroenterol Motil,2013; 25(5): e324-38.[J].Neurogastroenterol Motil, 2013, 25(5):e324-e338 [3]Furness JB.The enteric nervous system and neurogastroenterology[J]. Nat Rev Gastroenterol Hepatol. 2012,9(5):286-94.[J].Nat Rev Gastroenterol Hepatol, 2012, 9(5):286-294 [4]Sasselli V， Pachnis V， Burns AJ.The enteric nervous system[J]. Dev Biol. 2012,366(1):64-73.[J].Dev Biol, 2012, 366(1):64-73 [5]Hoff S， Zeller F， von Weyhern CW， Wegner M，Schemann M， Michel K， Rühl A.Quantitative assessment of glial cells in the human and guinea pig enteric nervous system with an anti-Sox8/9/10 antibody[J]. J Comp Neurol. 2008,509(4):356-71.[J].J Comp Neurol, 2008, 509(4):356-371 [6] Cirillo C, Sarnelli G, Esposito G, Turco F, Steardo L, Cuomo R．S100B protein in the gut:The evidence for enteroglial-sustained intestinal inflammation[J]．World J Gastroenterol.2011,17(10):1261-6.[J].World J Gastroenterol, 2011, 17(10):1261-1266 [7]Grundy D， Al-Chaer ED， Aziz Q， Collins SM， Ke M， Taché Y， Wood JD.Fundamentals of neurogastroenterology:basic science[J]. Gastroenterology. 2006,130(5):1391-411.[J].Gastroenterology, 2006, 130(5):1391-1411 [8]Bassotti G， Villanacci V， Fisogni S， Rossi E， Baronio P， Clerici C， Maurer CA， Cathomas G， Antonelli E.Enteric glial cells and their role in gastrointestinal motor abnormalities: introducing the neuro-gliopathies[J]. World J Gastroenterol. 2007,13(30):4035-41.[J].World J Gastroenterol, 2007, 13(30):4035-4041 [9]De Giorgio R， Giancola F， Boschetti E， Abdo H，Lardeux B， Neunlist M.Enteric glia and neuroprotection:basic and clinical aspects[J].Am J Physiol Gastrointest Liver Physiol, 2012, 303(8):G887-93 [10]Cheadle GA, Costantini TW, Bansal V, Eliceiri BP, Coimbra R.Cholinergic Signaling in the Gut: A Novel Mechanism of Barrier Protection through Activation of Enteric Glia Cells[J].Surg Infect (Larchmt), 2014, 15(4):387-93 [11] Gallego M, Olah I, Del Cacho E, Glick B.Anti-S-100 antibody recognizes[J].Dev Comp Immunol, 1993, 17(1):77-83 [12]ellipsoid-associated cells and other dendritic cells in the chicken spleen[J].Dev Comp Immunol,1993,17(1):77-83.[J].Neuroscience, 1981, 6(3):425-436 [13]A. Rühl.Glial cells in the gut[J].Neurogastroenterology & Motility, 2005, 17(6):777-790 [14]Neunlist M, Aubert P, Bonnaud S, Van Landeghem L, Coron E, Wedel T, Naveilhan P, Ruhl A, Lardeux B, Savidge T, Paris F, Galmiche JP.RühlGlial cells in the gut[J].Neurogastroenterology & Motility, 2007, 292(1):G234-G241 [15] Neunlist M, Aubert P, Bonnaud S, Van Landeghem L, Coron E, Wedel T, Naveilhan P, Ruhl A, Lardeux B, Savidge T, Paris F, Galmiche JP.Enteric glia inhibit intestinal epithelial cell proliferation partly through a TGF-beta1-dependent pathway,2007,292(1):G231-41.[J].Am J Physiol Gastrointest Liver Physiol, 2007, 292(1):G231-G241 [16] Furness, John Barton.The Enteric Nervous System[M]. Wiley-Blackwell，2006,288. [17]Gabella G.Fall in the number of myenteric neurons in aging guinea pigs[J].Gastroenterology, 1989, 96(6):1487-93 [18] Cabarrocas J, Savidge TC, Liblau RS.Role of enteric glial cells in inflammatory bowel disease[J]. Glia. 2003,41(1):81-93.[J].Gastroenterology, 2007, 132(4):1344-1358 [19] Savidge TC, Newman P, Pothoulakis C, Ruhl A, Neunlist M, Bourreille A, Hurst R, Sofroniew MV.Enteric glia regulate intestinal barrier function and inflammation via release of S-nitrosoglutathione[J]. Gastroenterology. 2007 Apr;132(4):1344-58.[J].Gastroenterology, 2009, 136(4):1349-1358 [20] Gulbransen BD, Sharkey KA.Purinergic neuron-to-glia signaling in the enteric nervous system[J]. Gastroenterology. 2009,136(4):1349-58.[J].Nat Rev Gastroenterol Hepatol, 2012, 9(11):625-632 [21]Gulbransen BD, Sharkey KA.Novel functional roles for enteric glia in the gastrointestinal tract[J].Nat Rev Gastroenterol Hepatol, 2012, 9(11):625-32 [22]Flamant M1, Aubert P, Rolli-Derkinderen M, Bourreille A, Neunlist MR, Mahé MM, Meurette G, Marteyn B, Savidge T, Galmiche JP, Sansonetti PJ, Neunlist M.Enteric glia protect against Shigella flexneri invasion in intestinal epithelial cells: a role for S-nitrosoglutathione[J].Gut, 2011, 60(4):473-84 [23]Neunlist M1, Aubert P, Bonnaud S, Van Landeghem L, Coron E, Wedel T, Naveilhan P, Ruhl A, Lardeux B, Savidge T, Paris F, Galmiche JP.Enteric glia inhibit intestinal epithelial cell proliferation partly through a TGF-beta1-dependent pathway[J].Am J Physiol Gastrointest Liver Physiol, 2007, 292(1):G231-41 [24]Greene DA, Lattimer SA, Sima AA.Are disturbances of sorbitol， phosphoinositide，and Na+-K+-ATPase regulation involved in pathogenesis of diabetic neuropathy[J].Diabetes, 1988, 37(6):688-93 [25]von Boyen GB, Steinkamp M, Reinshagen M, Sch?fer KH, Adler G, Kirsch J.Proinflammatory cytokines increase glial fibrillary acidic protein expression in enteric glia[J].Gut, 2004, 53(2):222-8 [26]Michetti F, Gazzolo D.S100B protein in biological fluids: a tool for perinatal medicine[J].Clin Chem, 2002, 48(12):2097-104 [27]Zimmer DB, Cornwall EH, Landar A, Song W.The S100 protein family: history,function,and expression[J].Brain Res Bull, 1995, 37(4):417-29 [28]Hoff S, Zeller F, von Weyhern CW, Wegner M, Schemann M, Michel K, Rühl A.Quantitative assessment of glial cells in the human and guinea pig enteric nervous system with an anti-Sox8910 antibody[J].J Comp Neurol, 2008, 509(4):356-71