诱导多潜能干细胞增殖分化能力强，既能避免免疫排斥, 又不涉及伦理道德问题, 而且取材方便，因而有望成为细胞移植治疗缺血性脑损伤的备选细胞。但近来的实验研究发现，诱导多潜能干细胞脑内移植后成瘤的可能性比较大，将其在体外分化为神经前体细胞，再进行移植治疗脑损伤应能有效避免其成瘤性。为此，本研究采用N2B27作为选择培养基，多步法贴壁培养把诱导多潜能干细胞诱导为神经前体细胞。免疫荧光染色发现该细胞神经干细胞的标记物nestin阳性，有极个别的β-Tub-Ⅲ阳性的细胞。在分化培养基内培养7天后，β-Tub-Ⅲ阳性的细胞增多。本实验初步表明诱导多潜能干细胞在体外可以被诱导为有一定神经特征的神经前体细胞，该细胞有望用于细胞移植治疗缺血性脑损伤。

The induced pluripotent stem cells (iPSCs) are expected to be candidate cells for cell transplantation in treatment of ischemic brain injury since they have a strong ability to proliferate and differentiate, and an easy accessibility without immune rejection and ethical issues. However, recent experiments have found that the iPSCs transplanted into the brain almost form tumors. Inducing the iPSCs into neural precursor cells (NPCs) in vitro before tansplantation may avoid tumor formation in the brain. The present study therefore has used the N2B27 as medium in a multistep adherent culture to induce the iPSCs into the NPCs. Immunofluorescence staining showed that the neural stem cell marker nestin was positive in the NPCs, in which there were very fewβ-Tub-III positive cells. After 7 days culture in the differentiation culture medium, β-Tub-III positive cells increased. This experiment has confiemrd that the iPSCs can be induced into the NPCs in vitro, which are expected to be used in the treatment of ischemic brain injury.