斑马鱼3D解剖互动系统的制作

林金杏; 章琳俐; 胡建华; 高诚; 冯丽萍; 魏晓锋; 陈微; 陈秋生

解剖学报 ›› 2016, Vol. 47 ›› Issue (1) : 139-144.

技术方法

斑马鱼3D解剖互动系统的制作

林金杏¹,章琳俐²,胡建华¹,高诚¹,冯丽萍¹,魏晓锋¹,陈微²,陈秋生³

作者信息 +

Established the 3D anatomy interaction system of zebrafish (Danio rerio)

Author information +

文章历史 +

摘要

目的旨在制作斑马鱼三维（three-dimension, 3D）解剖互动系统软件。方法采用光镜和Micro-CT的方法研究了斑马鱼的组织构造，并收集其形态结构图像。使用Photoshop对获得的连续切片（HE染色）的图片进行校正，使用Autodesk 3ds Max软件进行三维模型建立。结果获得了斑马鱼骨骼的3D成像和连续切片图像（雄鱼144张，雌鱼179张），实现了斑马鱼重要器官的3D重构，建立了斑马鱼3D解剖互动系统(V 1.0)，获得了计算机软件著作权登记证书（证书号：软著登字第0989966号）。结论斑马鱼3D解剖互动系统软件制作的完成为研究斑马鱼立体形态学奠定了基础，有助于研究人员掌握斑马鱼的组织形态。

Abstract

Objective The aim of this paper was to make a software of three-dimension (3D) anatomical interaction system in zebrafish (Danio rerio). Methods The structure of zebrafish was observed by light microscopy and Micro-CT, and the images of its morphological structure were collected. The pictures of serial section (HE-stained) were corrected by Photoshop. The 3D model was established by the software of Autodesk 3ds Max. Results The results showed the zebrafish skeletal 3D imaging and serial section images (male: 144, female: 179). We realized the 3D reconstruction of the vital organs of zebrafish, and established the 3D anatomy interaction system zebrafish (V 1.0), which obtained the computer software copyright registration certificate (Certificate Number: soft word board the Di_0989966). Conclusion The zebrafish 3D anatomy interaction system software afforded the three-dimensional morphological basis for the study of zebrafish, and was helpful to histomorphology recognization for researchers.

导出引用

林金杏章琳俐胡建华高诚冯丽萍魏晓锋陈微陈秋生. 斑马鱼3D解剖互动系统的制作[J]. 解剖学报. 2016, 47(1): 139-144

Established the 3D anatomy interaction system of zebrafish (Danio rerio)[J]. Acta Anatomica Sinica. 2016, 47(1): 139-144

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