[摘要] 目的 研究白藜芦醇对大鼠脑缺血再灌注损伤后突触素表达的影响。方法 60只SD雄性大鼠随机分成假手术组(Sham)、缺血对照组(Con)和白藜芦醇组(Res)，每组20只。缺血3 h后腹腔注射白藜芦醇或无水乙醇，连用7 d。脑缺血24 h时，TTC法测量脑梗死体积，干湿称重法检测脑含水量。缺血24 h、7 d、14 d分别行Bederson神经功能缺损评分、免疫组化法及Western blot检测突触素蛋白的表达。结果 TTC染色显示对照组和白藜芦醇组梗死体积较假手术组高（P < 0. 05），而白藜芦醇组较对照组显著减小(P < 0. 05)。脑含水量检测显示对照组和白藜芦醇组较假手术组明显增高（P < 0. 05），但白藜芦醇组较对照组显著降低（P< 0. 05）。神经功能缺损评分显示24 h、7 d、14 d对照组和白藜芦醇组显著高于假手术组（P < 0. 05），但白藜芦醇组较对照组显著降低（P < 0. 05）。免疫组化显示对照组24h时脑缺血皮质突触素阳性表达减少，7 d 、14 d时逐渐增加，但均较假手术组显著减少。白藜芦醇组各时间点均较对照组明显增加，14 d增加更明显。WB显示对照组24 h、7 d、14 d脑缺血区皮质突触素蛋白表达较假手术组显著降低（P < 0. 05），但7 d 、14 d时逐渐增加；白藜芦醇组各时间点较缺血对照组显著增强（P < 0. 05），14 d时增加更显著。结论 白藜芦醇治疗可增强脑缺血大鼠突触素的表达，改善神经功能。
Abstract
[Abstract] Objective To examinate the effect of resveratrol on expression of synaptophysin after cerebral ischemia/reperfusion injury in rats. Methods 60 male Sprague-Dawley rats were divided into sham, ischemic control and resveratrol group. Each group is 20 rats. The rats were treated with resveratrol or ethyl alcohol by intraperitoneally for 7 consecutive days after 3 h for MCAO. At 24 h after MCAO/R, TTC assay examinated cerebral infarction volume and dry and wet weight method tested moisture content in brain tissue. At 24 h, 7 d and 14 d after MCAO/R, we examinated the neurological function score with Bederson score and the protein expression of synaptophysin in ischemic cortex with immunohistochemistry and Western blot. Results The infarction volumes in the control and resveratrol group were significantly higher than in the sham group (P < 0. 05), and it was significantly lower in the resveratrol group than in the control group (P < 0. 05).Brain water contents in the control and resveratrol group were significantly higher than in the sham group (P < 0. 05), and it was significantly lower in the resveratrol group than in the control group (P < 0. 05). The neurological function score in the control and resveratrol group were significantly higher than in the sham group at 24 h, 7 d and 14 d (P < 0. 05), and it was significantly lower in the resveratrol group than in the control group (P < 0. 05). Immunohistochemistry display that the positive expression of synaptophysin in ischemic cortex in the control group were reduced at 24 h, and increased gradually at 7 d and 14 d, but were significantly lower than in the sham group. After resveratrol treatment, it was significantly higher than in the control group at each point time, and was highest at 14 d. Western blot assay showed that the protein expression of synaptophysin in the control group was significantly lower than in the sham group at 24 h, 7 d and 14 d after MCAO/R (P < 0. 05), but gradually increased at 7 d and 14 d. After resveratrol treatment, it was significantly enhanced than in the control group at each point time (P < 0. 05), and was strongest at 14 d. Conclusion Resveratrol treatment can enhance the expression of synaptophysin and improve neurological function.
关键词
白藜芦醇 /
脑缺血再灌注 /
突触素 /
表达 /
大鼠
Key words
Resveratrol /
Cerebral ischemia/reperfusion /
Synaptophysin /
Expression /
Rat
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基金
国家自然科学基金面上项目;卫计委国家临床重点专科建设项目资金资助
