目的 探讨不同丹参注射液剂量对冻融小鼠卵巢移植早期卵泡发育影响的研究。方法 收集1日龄小鼠卵巢，慢冻速融后移植至F1代8-12周雄鼠的肾被膜下，分生理盐水组和低(0.4ml/kg)、中(4ml/kg)、高(40ml/kg)剂量丹参干预组，每组15只，分别于移植后第二天（2d）、第七天（7d）和第十四天（14d）回收12组卵巢移植物。观察冻融卵巢组织移植前后的组织形态学及超微结构改变、计数卵泡数量。 结果 光、电镜观察各组卵巢组织移植后7d卵泡细胞和颗粒细胞结构欠完整，移植后14d卵泡结构恢复正常。移植后14d高剂量丹参组（36.2%)各级卵泡数及卵泡存活率多于中、低剂量丹参组（24.2%、20.5)、生理盐水组（12.6%），差异显著（P＜0.05）。结论 结合光镜和电镜观察移植早期的缺血缺氧对小鼠卵巢组织造成了远比冻融过程更严重的损伤；丹参能促进移植后冻融小鼠卵巢卵泡发育，以高剂量丹参作用明显。

Objective To investigate the different doses Salvia miltiorrhiza promote follicular development in frozen-thawed mouse ovarian in an early stage after transplantation. Methods Ovaries from 1 day old mouse were cryopreserved and the thawed ovaries were xenografted into kidney capsules of 8~12 weeks old adult male mouse. The mouse were divided into four groups: Saline group、low doses(0.4ml/kg) Salvia miltiorrhiza group、middle doses (4ml/kg)Salvia miltiorrhiza group and high doses (40ml/kg) Salvia miltiorrhiza group. The grafted ovaries were collected 2, 7, and 14 days after transplantation for evaluating. Observed the histological structure and the ultrastructural changes、counted follicle numbers of the mouse ovaries after frozen-thawed and transplantation. Results The histological structure and ultrastructure showed that both the follicles and stromata were damaged on day 7 after transplantation. The follicular structure have been recovered for 14 days. The number of follicles and survival rates in grafts after transplantation of high doses Salvia miltiorrhiza group(36.2%) were more than that of middle doses、low dose Salvia miltiorrhiza group and Saline group（（24.2%、20.5、12.6%）on day 14（P＜0.05）. Conclusions Both the histological observation and ultrastructure observation methods were used in present study analyze the injury of the mouse ovarian tissues at the early stage is due to the ischemia and hypoxia rather than the cryopreservation. High doses Salvia miltiorrhiza may provide benefits for the survival rates of follicles in the frozen-thawed mouse ovarian.