Objective To observe and analyze the expression of fasciculation and elongation protein zeta-1 (FEZ1) gene in purified type-1 astrocytes (T1A) and type-2 astrocytes (T2A). Methods By the technique of gene-chip, some related genes in the different gene expression profiles of purified T1A and T2A were compared and analyzed. Increased mRNA levels of FEZ1 in T2A was further confirmed by RT-PCR. Results 138 different genes were differentially expressed in T1A and T2A, 60 of which were highly expressed in T1A and 78 in T2A. The expression of FEZ1 was up-regulated in T2A, and the expression of FEZ1 was detected in the different parts (cerebral cortex, olfactory bulb and spinal cord) of the central nervous system of P1 rats.Conclusion FEZ1 gene is among several genes that expressed differently in T1A and T2A. The expression of FEZ1 is found in central nervous system of P1 rats. FEZ1 may play an important role in the development and regeneration of central nervous system.