Objective To study the expression and clinical significance of antyapoptosis protein BAG-1 in nonsmall cell lung cancer（NSCLC）,and its relationship with cell apoptosis and expression of Bcl-2 protein. Methods Immunohisto chemistry streptavidinperoxidase conjugated (SP)method was used to examine the expression of BAG-1protein and Bcl-2 protein, and terminal deoxynucleotidyl transferase mediated UTP nick end labeling (TUNEL) method was used to examine the apoptosis index in 54 cases of NSCLC .The expression of BAG-1 protein in 20 cases of normal bronchus mucosa tissue also be detected as control. Results Express positive rate of BAG-1 protein in NSCLC is 7407%, obviously higher than that in normal bronchus mucosa tissue (positive rate is 5%). In cases of NSCLC ,the expression of BAG-1 protein has not correlation with the age, gender, pathologic classification,but have closed correlation with lymph node metastasis，degree of differentiation,pTNM stage(P<0.05).There is positive correlation betweened BAG-1 protein expression and Bcl-2 protein expression（pearson contingency coefficient is 0.406,P=0.003，P<0.01）.Following the increasing of expression degree of BAG-1protein,the apoptosis index of cancer cell was degraded. The statistically significant difference was found between each groups. Conclusion BAG-1 protein is over expression in NSCLC compare with normal bronchus mucosa tissue,have closed relation with NSCLC genesis and development.There is posi